CAJ | 학술논문

以分离自广东省镇海水库的拟柱孢藻 N8 为对象, 探究其在不同磷浓度及氮磷浓度组合下的生长情况.结果表明,拟柱孢藻N8对磷的适应范围很宽,在0.02—5.12 mg/L磷浓度下均能生长,最适生长磷浓度范围为0.16—5.12 mg/L,磷浓度的升高能显著延长拟柱孢藻的对数生长期和提高生物量.动力学分析表明,拟柱孢藻 N8 有较低的 KSP值, 对磷元素的亲和性较高, 在磷营养贫乏的环境下更容易形成优势.在氮磷组合实验中,低氮(0.5 mg/L)显著抑制拟柱孢藻的生长,且这种生长抑制不受磷浓度的影响;而在低磷(0.04 mg/L)条件下,水体中氮浓度的增加会显著促进拟柱孢藻的生长,拟柱孢藻在高氮中磷和高氮高磷下的生长显著优于其他氮磷组合条件.研究表明, 广东省水库拟柱孢藻的生长受磷的限制较弱, 氮是其生长的决定因子.
이분리자광동성진해수고적의주포조 N8 위대상, 탐구기재불동린농도급담린농도조합하적생장정황.결과표명,의주포조N8대린적괄응범위흔관,재0.02—5.12 mg/L린농도하균능생장,최괄생장린농도범위위0.16—5.12 mg/L,린농도적승고능현저연장의주포조적대수생장기화제고생물량.동역학분석표명,의주포조 N8 유교저적 KSP치, 대린원소적친화성교고, 재린영양빈핍적배경하경용역형성우세.재담린조합실험중,저담(0.5 mg/L)현저억제의주포조적생장,차저충생장억제불수린농도적영향;이재저린(0.04 mg/L)조건하,수체중담농도적증가회현저촉진의주포조적생장,의주포조재고담중린화고담고린하적생장현저우우기타담린조합조건.연구표명, 광동성수고의주포조적생장수린적한제교약, 담시기생장적결정인자.
In this study we examined the effects of nitrate and phosphate on the growth ofCylindrospermopsis racibor-skiiN8 that was isolated from the Zhenhai Reservoir in Guangdong Province.We used a crossed factorial design for three phosphorus levels and three nitrogen levels, and thus generated nine different growth conditions forC. raciborskii. Our results showed thatC. raciborskii could adapt to a wide range of phosphorus concentrations (from 0.02 mg/mL to 5.12 mg/mL) and the optimal concentrations for the growth was between 0.16 mg/mL and 5.12 mg/mL. The exponential stage and biomass of C. raciborskii could be significantly enhanced by the increase in the phosphorus level. The kinetic analysis revealed a lowKSP value of C. raciborskii N8. This suggested thatC. raciborskii N8 could have high affinity for phosphorus and readily become the dominant species in phosphorus-lacking conditions. The results of the nitrogen and phosphorus orthogonal experiments demonstrated that when the nitrogen level was low (0.5 mg/mL), the growth of C. raciborskii N8 was markedly inhibited regardless of the phosphorus level. Moreover, at low phosphorus concentra-tion (0.04 mg/mL) the proliferation ofC. raciborskii N8 could be boosted by the increase in nitrogen concentration. It was also found that the growth ofC. raciborskii N8 could be significantly improved by HNMP and HNHP treatment. The highlights of our findings were thatC. raciborskii in Guangdong reservoirs was highly tolerant to low phosphorus conditions, and nitrogen could be a key factor in its growth.