北华大学学报(自然科学版)
北華大學學報(自然科學版)
북화대학학보(자연과학판)
JOURNAL OF BEIHUA UNIVERSITY(NATURAL SCIENCE)
2015年
4期
462-464
,共3页
肠癌%肠道菌群%基因组DNA
腸癌%腸道菌群%基因組DNA
장암%장도균군%기인조DNA
colon cancer%intestinal bacteria%genomic DNA
目的:探讨肠道肿瘤患者与健康人群肠道菌群构成及基因组DNA的变化特征.方法分别采集肠癌患者与健康人晨起粪便,进行需氧和厌氧培养,鉴定菌株并进行菌落计数.采用试剂盒法提取粪便中肠道细菌总基因组DNA,进行琼脂糖凝胶电泳.结果肠癌组与健康对照组比较,肠杆菌数量增多,以大肠埃希菌数量显著,乳酸杆菌和双歧杆菌明显减少,厌氧菌和需氧菌的数量比例颠倒,两组数据比较差异均具有统计学意义(P<0.05).粪便总基因组DNA经琼脂糖凝胶电泳在23130 bp处有清晰、明亮的单一条带,且肠癌组与健康对照组均有相同大小的条带.结论肠癌组与健康对照组肠道菌群组成存在差异,肠道菌群总基因组DNA没有变化.
目的:探討腸道腫瘤患者與健康人群腸道菌群構成及基因組DNA的變化特徵.方法分彆採集腸癌患者與健康人晨起糞便,進行需氧和厭氧培養,鑒定菌株併進行菌落計數.採用試劑盒法提取糞便中腸道細菌總基因組DNA,進行瓊脂糖凝膠電泳.結果腸癌組與健康對照組比較,腸桿菌數量增多,以大腸埃希菌數量顯著,乳痠桿菌和雙歧桿菌明顯減少,厭氧菌和需氧菌的數量比例顛倒,兩組數據比較差異均具有統計學意義(P<0.05).糞便總基因組DNA經瓊脂糖凝膠電泳在23130 bp處有清晰、明亮的單一條帶,且腸癌組與健康對照組均有相同大小的條帶.結論腸癌組與健康對照組腸道菌群組成存在差異,腸道菌群總基因組DNA沒有變化.
목적:탐토장도종류환자여건강인군장도균군구성급기인조DNA적변화특정.방법분별채집장암환자여건강인신기분편,진행수양화염양배양,감정균주병진행균락계수.채용시제합법제취분편중장도세균총기인조DNA,진행경지당응효전영.결과장암조여건강대조조비교,장간균수량증다,이대장애희균수량현저,유산간균화쌍기간균명현감소,염양균화수양균적수량비례전도,량조수거비교차이균구유통계학의의(P<0.05).분편총기인조DNA경경지당응효전영재23130 bp처유청석、명량적단일조대,차장암조여건강대조조균유상동대소적조대.결론장암조여건강대조조장도균군조성존재차이,장도균군총기인조DNA몰유변화.
Objective To explore differences in the composition of intestinal flora between the colorectal cancer patients and the healthy group in order to find an alternative method for the early prognosis of colorectal cancer. Method Early morning stool of the cancer patients and the healthy people were collected. Aerobic and anaerobic cultures were carried out,the strains were identified,and the bacterial colonies were count. Bacterial genomic DNA in feces was extraced from the colorectal cancer and the healthy group,respectively,and underwent agarose gel electrophoresis. Results Compared the cancer group with the control group, the amount of E. coli increased significantly,the amount of lactobacillus and bifidobacterium reduced significantly,and the proportional quantity of anaerobic bacteria and aerobic bacteria reversed. All the datum showed statistically significant (P<0. 05). There was a clear and bright single band at 23 130 bp on the agarose gel electrophoresis. Conclusion There were differences in intestinal flora composition between the cancer patients and the healthy control group, but their total genomic DNA was the same.