CAJ | 학술논문

目的：探讨桉柠蒎与慢性组塞性肺疾病（ COPD）大鼠肺功能及气道细菌负荷的影响。方法：将雄性Wistar大鼠120只分为对照组（正常喂养12周）、模型组、预防组和治疗组，后3组给予熏烟12周，预防组大鼠在熏烟前30 min给予桉柠蒎灌胃，治疗组大鼠熏烟第9周后给予桉柠蒎灌胃，烟熏12周后，通过小动物肺功能议检测第0.3秒用力呼气容积与用力肺活量的百分比（ FEV0.3/FVC）、功能残气量（ FRC）等肺功能指标；将4组大鼠统一气管滴入流感嗜血杆菌混悬液，并于接种后3h、6h、12h和24h时取其左肺胞灌洗液和右肺组织匀浆接种于巧克力培养基培养，计数细菌菌落。结果：预防组和治疗组FRC明显低于模型组，预防组改善更为显著（P＜0.01）；模型组的支气管肺泡灌洗液（ BALF）和肺组织匀浆的菌落计数在各时间点均明显高于对照组（ P＜0.01）；预防组和治疗组的 BALF和肺组织匀浆的菌落计数较模型组明显降低，且预防组下降更为显著（P ＜0.01）；各组菌落计数均于种菌后6 h时达到高峰，种菌后3 h、12 h、24 h时的肺匀浆菌落计数与FRC呈正相关（P＜0.01），FEV0.3/FVC与6 h的肺匀浆菌落计数呈负相关（P＜0.05）。结论：桉柠蒎能改善肺泡过度充气，但短期内应用不能延缓肺功能的下降趋势或改善肺功能，桉柠蒎可以降低COPD大鼠气道的细菌负荷，预防给药效果更优；气道细菌负荷受肺功能的影响，肺功能越差，细菌负荷越重。
목적：탐토안저파여만성조새성폐질병（ COPD）대서폐공능급기도세균부하적영향。방법：장웅성Wistar대서120지분위대조조（정상위양12주）、모형조、예방조화치료조，후3조급여훈연12주，예방조대서재훈연전30 min급여안저파관위，치료조대서훈연제9주후급여안저파관위，연훈12주후，통과소동물폐공능의검측제0.3초용력호기용적여용력폐활량적백분비（ FEV0.3/FVC）、공능잔기량（ FRC）등폐공능지표；장4조대서통일기관적입류감기혈간균혼현액，병우접충후3h、6h、12h화24h시취기좌폐포관세액화우폐조직균장접충우교극력배양기배양，계수세균균락。결과：예방조화치료조FRC명현저우모형조，예방조개선경위현저（P＜0.01）；모형조적지기관폐포관세액（ BALF）화폐조직균장적균락계수재각시간점균명현고우대조조（ P＜0.01）；예방조화치료조적 BALF화폐조직균장적균락계수교모형조명현강저，차예방조하강경위현저（P ＜0.01）；각조균락계수균우충균후6 h시체도고봉，충균후3 h、12 h、24 h시적폐균장균락계수여FRC정정상관（P＜0.01），FEV0.3/FVC여6 h적폐균장균락계수정부상관（P＜0.05）。결론：안저파능개선폐포과도충기，단단기내응용불능연완폐공능적하강추세혹개선폐공능，안저파가이강저COPD대서기도적세균부하，예방급약효과경우；기도세균부하수폐공능적영향，폐공능월차，세균부하월중。
Objective:To investigate the effect of eucalyptol on pulmonary function and airway bacte-rial load in COPD rats. Methods:A total of 120 rats were randomly divided into four groups:control group,model group,prevention group and treatment group. The last three groups were exposed to cig-arettes for 12 weeks. Prevention group was administered intragastric infusion with Eucalyptol 30 min before exposure to cigarettes;treatment group was administered intragastric infusion with Eucalyptol 9 weeks after exposure to cigarettes. After 12 weeks,the data of FEV0. 3/FVC and FRC were detected by small animal lung function analysis system. After that,the rats received intratracheal instillation with Haemophilus influenzae suspension. Collecting left pulmonary alveolus lavage fluid and right lung homogenate after 3 h,6 h,12 h,24 h,then inoculated on chocolate agar to cultivate and count bacte-rial clump. Results:The detection of lung histopathology and the results of pulmonary function test re-vealed the model were consistent with COPD diagnosis. FRC in prevention and treatment group de-creased significantly comparing with model group,improvement in prevention group was more obvious. BALF and lung homogenate bacterial clump in model group was higher than that of control group in ev-ery recorded time spot. BALF and lung homogenate bacterial clump in prevention and treatment group were lower than that of model group,especially prevention group decreased significantly(P<0. 01). Bacterial clump count from each group reached peak at 6 h;there was a positive correlation between lung homogenate bacterial clump count 3 h,12 h,24 h with FRC(P<0. 01),there was a negative correlation between FEV0. 3/FVC and 6 h lung homogenate bacterial clump count. Conclusion:Euca-lyptol can improve the over-inflation of alveoli,but it cannot postpone the decrease of pulmonary func-tion or improve it. Eucalyptol decreases the airway bacterial load in COPD rats,and there is a better effect in eucalyptol prevention medication. Bacterial load is influenced by the pulmonary function.