CAJ | 학술논문

目的：探讨白细胞介素2（IL-2）以及肿瘤坏死因子-α（TNF-α）基因启动子SNP位点与丙型肝炎病毒（ HCV）慢性感染的相关性。方法：选择云南地区汉族人群HCV慢性感染患者380例，健康体检人群367例，采用TaqMan探针基因分型方法对IL-2基因启动子SNPrs2069762、rs2069763和rs4833248，以及TNF-α基因启动子SNPrs1800629、rs3093668和rs3093726进行基因分型，并构建单倍型，统计IL-2和TNF-α多态性位点的等位基因频率与基因型频率、单倍型频率，分析SNPs及单倍型与HCV慢性感染的相关性。结果：IL-2基因启动子中，病例组和对照组相比，SNPrs2069762基因型差异有统计学意义（ P＜0.05，OR=0.864，95%CI为0.700～1.066），病例组CC基因型频率高于对照组（ P＜0.05），rs2069763等位基因差异具有统计学意义（ P＜0.05，OR=1.234，95％CI为1.007～1.513），病例组等位基因G频率高于对照组（P＜0.05），rs4833248基因型和等位基因频率差异无统计学意义（P＞0.05），rs2069762/rs2069763/rs4833248单倍型ATG差异有统计学意义（P＜0.05，OR=0.812，95％CI 为0.662～0.996），病例组单倍型 ATG 频率低于对照组（ P ＜0.05）；TNF-α基因启动子SNPrs1800629、rs3093668和rs3093726基因型、等位基因型和单倍型频率，在病例组和对照组中差异无统计学意义（P＞0.05）。结论：在云南汉族群体中，IL-2基因启动子SNPrs2069762 CC基因型和rs2069763 G等位基因可能是HCV慢性感染的易感因素，rs2069762/rs2069763/rs4833248单倍型ATG可能是HCV慢性感染的保护性因素；TNF-α基因启动子SNPrs1800629、rs3093668和rs3093726与HCV慢性感染没有相关性。
목적：탐토백세포개소2（IL-2）이급종류배사인자-α（TNF-α）기인계동자SNP위점여병형간염병독（ HCV）만성감염적상관성。방법：선택운남지구한족인군HCV만성감염환자380례，건강체검인군367례，채용TaqMan탐침기인분형방법대IL-2기인계동자SNPrs2069762、rs2069763화rs4833248，이급TNF-α기인계동자SNPrs1800629、rs3093668화rs3093726진행기인분형，병구건단배형，통계IL-2화TNF-α다태성위점적등위기인빈솔여기인형빈솔、단배형빈솔，분석SNPs급단배형여HCV만성감염적상관성。결과：IL-2기인계동자중，병례조화대조조상비，SNPrs2069762기인형차이유통계학의의（ P＜0.05，OR=0.864，95%CI위0.700～1.066），병례조CC기인형빈솔고우대조조（ P＜0.05），rs2069763등위기인차이구유통계학의의（ P＜0.05，OR=1.234，95％CI위1.007～1.513），병례조등위기인G빈솔고우대조조（P＜0.05），rs4833248기인형화등위기인빈솔차이무통계학의의（P＞0.05），rs2069762/rs2069763/rs4833248단배형ATG차이유통계학의의（P＜0.05，OR=0.812，95％CI 위0.662～0.996），병례조단배형 ATG 빈솔저우대조조（ P ＜0.05）；TNF-α기인계동자SNPrs1800629、rs3093668화rs3093726기인형、등위기인형화단배형빈솔，재병례조화대조조중차이무통계학의의（P＞0.05）。결론：재운남한족군체중，IL-2기인계동자SNPrs2069762 CC기인형화rs2069763 G등위기인가능시HCV만성감염적역감인소，rs2069762/rs2069763/rs4833248단배형ATG가능시HCV만성감염적보호성인소；TNF-α기인계동자SNPrs1800629、rs3093668화rs3093726여HCV만성감염몰유상관성。
Objective:To investigate the correlation among HCV chronic infection and SNPs in IL-2 and TNF-α gene promoter. Method:A total of 380 patients with HCV chronic infection and 367 Healthy controls of Yunnan Han people were selected. TaqMan probe genotyping was applied to detect the IL-2 gene promoter SNP rs2069762 ,rs2069763 and rs4833248 ,coupled with TNF-αgene promot-er SNP rs1800629,rs3093668,rs3093726,then constructing haplotype. Estimating allele frequency and genotype frequency,and haplotype frequency of IL-2 and TNF-αSNP. Then analyzing correlation between SNPs and haplotype AGT and HCV chronic infection. Results:Concerning IL-2 gene promot-er of infection group and control group,SNP rs2069762 genotypic variation showed significant differ-ence( P<0 . 05 ,OR =0 . 864 ,95% CI:0 . 700 ~1 . 066 ),and allele frequency of genotype CC in HCV chronic infection patients was higher than that of control group( P<0 . 05 );the rs2069763 allele showed a significant difference( P<0 . 05 ,OR=1 . 234 ,95% CI1 . 007~1 . 513 )and the allele G fre-quency of infection group was higher than that of control group( P<0 . 05 );the genotype and allele fre-quency of rs4833248 displayed no significant difference ( P > 0. 05 );rs2069762/ rs2069763/rs4833248 haplotype ATG showed significant difference( P <0 . 05 ,OR =0 . 812 ,95%CI:0 . 662 ~0. 996 )and haplotype ATG frequency of infection group was lower than control group( P <0 . 05 ). Genotype,allele and haplotype frequency of rs1800629,rs3093668,rs3093726 in TNF-αhad no sta-tistical significance in both groups(P>0. 05). Conclusions:For Han people in Yunnan province, the rs2069762 genotype CC and rs2069763 allele G in IL-2 are potential predisposing factors of HCV chronic infection. rs2069762/ rs2069763/ rs4833248 haplotype ATG might play a protective role in HCV chronic infection. SNP rs1800629,rs3093668,rs3093726 in TNF-αgene promoter show no cor-relation with HCV chronic infection.