CAJ | 학술논문

目的：探讨苯代谢物氢醌（ HQ）对人白血病细胞株K562细胞中着色性干皮病基因D（ XPD）mRNA转录及蛋白表达的影响。方法：分别用终浓度为0、15、30、60μmol/L HQ溶液处理K562细胞48 h后，单细胞凝胶电泳实验检测细胞DNA损伤效应，Taqman探针实时荧光定量PCR法检测XPD基因mRNA转录水平，蛋白印迹法分析XPD基因蛋白表达水平，观察荧光显微镜下细胞的尾矩，计算mRNA及蛋白的相对表达量。结果：不同浓度HQ处理后，各组细胞尾矩与0μmol/L HQ组比较，差异有统计学意义（ P＜0.05）；不同浓度HQ处理后， K562细胞中XPD基因mRNA和蛋白相对表达量与0μmol/L HQ组比较，差异无统计学意义（ P＞0.05）。结论：HQ处理K562细胞48 h后，细胞中DNA有明显的损伤效应，但XPD基因mRNA及蛋白无变化。
목적：탐토분대사물경곤（ HQ）대인백혈병세포주K562세포중착색성간피병기인D（ XPD）mRNA전록급단백표체적영향。방법：분별용종농도위0、15、30、60μmol/L HQ용액처리K562세포48 h후，단세포응효전영실험검측세포DNA손상효응，Taqman탐침실시형광정량PCR법검측XPD기인mRNA전록수평，단백인적법분석XPD기인단백표체수평，관찰형광현미경하세포적미구，계산mRNA급단백적상대표체량。결과：불동농도HQ처리후，각조세포미구여0μmol/L HQ조비교，차이유통계학의의（ P＜0.05）；불동농도HQ처리후， K562세포중XPD기인mRNA화단백상대표체량여0μmol/L HQ조비교，차이무통계학의의（ P＞0.05）。결론：HQ처리K562세포48 h후，세포중DNA유명현적손상효응，단XPD기인mRNA급단백무변화。
Objective:To explore effects of different concentration of hydroquinone( HQ)on mRNA transcription and protein expression of xeroderma pigmentosum group D( XPD )in Human leukemia cell line K562 cells. Methods:K562 cells were treated and contaminated with 0,15,30,60 μmol/L hydroquinone for 48h,respectively. The DNA damage effect of the cells exposed to hydroquinone was detected by single cell gel electrophoresis. The mRNA transcription of XPD was detected by Taq-man probe real-time fluorescence quantitative PCR. The protein expression of XPD was detected by Western blot. Results:In DNA damage effect experiment,there existed significant differences in the tail moment between contamination groups and control group(0 μmol/L hydroquinone)(P<0. 05). In experiments of mRNA transcription of XPD and protein expression of XPD,there was no significant difference in mRNA transcription of XPD and protein expression of XPD between contamination groups and control group(P>0. 05). Conclusion:After K562 cells are treated with hydroquinone for 48 h, the DNA damage effect of K562 cells is obvious. But the change of mRNA transcription and protein ex-pression of XPD can not be found.