CAJ | 학술논문

Maf蛋白家族作为重要的转录调控因子, 参与细胞众多生命进程.它分为大Maf蛋白和小Maf蛋白, 小Maf蛋白需要与其他B-Zip家族蛋白结合形成异二聚体才能发挥作用.在斑马鱼(Danio rerio)中,小Maf包括Mafk、Mafg1、Mafg2和Maft, Mafg1和Mafg2是Mafg在鱼类中特有的分化出的两个旁系同源基因.为了研究这4个小maf在物种之间的进化关系及表达模式, 实验对4个小maf进行进化树、染色体线性关系分析, 结果显示4个小maf的分子进化方向与物种进化方向一致, mafk和maff在物种间的更趋于保守, mafg比前两者要相对活跃.同时,实验选取斑马鱼胚胎发育十个不同时期(1 cell、2 cells、3.5 hpf、6 hpf、12 hpf、24 hpf、36 hpf、48 hpf、72 hpf、96 hpf),对其表达模式进行了详细的研究,结果显示4个小maf中,mafg2的表达量最高,maft和mafk的表达量次之,mafg1的表达量最低.在胚胎发育的后期(48 hpf),除了mafg2在躯干的血液循环处有明显的表达, maft在躯干的血液循环处有较弱的表达, 而除mafk和mafg1在血液循环处没有检测到表达之外, 4个基因的表达部位基本重叠,都在全身广泛表达,这可能也与它们之间存在功能叠加和替换有关.利用双荧光素酶检测系统检测了4个小Maf参与调控胰外分泌酶原基因转录的不同功能, 结果显示 4个小 maf的过表达都能使胰外分泌酶原基因的表达下调.研究结果将为深入研究 4个小 maf基因的功能叠加和互补作用奠定基础.
Maf단백가족작위중요적전록조공인자, 삼여세포음다생명진정.타분위대Maf단백화소Maf단백, 소Maf단백수요여기타B-Zip가족단백결합형성이이취체재능발휘작용.재반마어(Danio rerio)중,소Maf포괄Mafk、Mafg1、Mafg2화Maft, Mafg1화Mafg2시Mafg재어류중특유적분화출적량개방계동원기인.위료연구저4개소maf재물충지간적진화관계급표체모식, 실험대4개소maf진행진화수、염색체선성관계분석, 결과현시4개소maf적분자진화방향여물충진화방향일치, mafk화maff재물충간적경추우보수, mafg비전량자요상대활약.동시,실험선취반마어배태발육십개불동시기(1 cell、2 cells、3.5 hpf、6 hpf、12 hpf、24 hpf、36 hpf、48 hpf、72 hpf、96 hpf),대기표체모식진행료상세적연구,결과현시4개소maf중,mafg2적표체량최고,maft화mafk적표체량차지,mafg1적표체량최저.재배태발육적후기(48 hpf),제료mafg2재구간적혈액순배처유명현적표체, maft재구간적혈액순배처유교약적표체, 이제mafk화mafg1재혈액순배처몰유검측도표체지외, 4개기인적표체부위기본중첩,도재전신엄범표체,저가능야여타문지간존재공능첩가화체환유관.이용쌍형광소매검측계통검측료4개소Maf삼여조공이외분비매원기인전록적불동공능, 결과현시 4개소 maf적과표체도능사이외분비매원기인적표체하조.연구결과장위심입연구 4개소 maf기인적공능첩가화호보작용전정기출.
The members of musculoaponeurotic fibrosarcoma oncogene (Maf) family are important transcription factors that are involved in many cascades of cell biology. They are classified into large and small Maf proteins. The small Maf proteins of zebrafish include Mafk, Mafg1, Mafg2 and Maff (Maft).Mafg1 andmafg2 are paraloges ofmafg that only exist in fish genomes. The phylogenetic tree indicated that the evolution of the small Mafs was conservative. The systemic analyses of different smallmafs also showed thatmafk andmaff were more conservative thanmafg. In the present study we analyzed the spatial-temporal expression of these fourmafsin the early development of zebrafish em-bryos using semiquantitative PCR and whole mountin situ hybridization. The expression of all four genes started at the one cell stage, suggesting that these genes were maternal and the expression were ubiquitous in early zebrafish embryos. Mafg2 showed the highest expression level among the four genes, and was specifically expressed in blood circulation after 48 hpf, together withmaff. The results of dual-luciferase system also showed that the over expression of the four small Mafs led to down-regulation of the transcription of zymogens in a dose-dependent manner. Our study should con-tribute to better understanding of the functional superposition and complementarities of small Mafs.