中国中西医结合外科杂志
中國中西醫結閤外科雜誌
중국중서의결합외과잡지
CHINESE JOURNAL OF SURGERY OF INTEGRATED TRADITIONAL AND WESTERN MEDICINE
2015年
3期
227-230
,共4页
许丽萍%孙翠云%王新允%郑海燕
許麗萍%孫翠雲%王新允%鄭海燕
허려평%손취운%왕신윤%정해연
肺癌%肌肉细胞特异性泛素蛋白连接酶29蛋白%p40蛋白%甲状腺转录因子-1%鉴别诊断
肺癌%肌肉細胞特異性汎素蛋白連接酶29蛋白%p40蛋白%甲狀腺轉錄因子-1%鑒彆診斷
폐암%기육세포특이성범소단백련접매29단백%p40단백%갑상선전록인자-1%감별진단
Lung tumor%tripartite motif containing 29%p40%TTF-1%differential diagnosis
目的：探讨肌肉细胞特异性泛素蛋白连接酶29(TRIM29)、p40蛋白和甲状腺转录因子-1（TTF-1）联合应用在肺鳞癌和肺腺癌鉴别诊断中的价值。方法：应用组织芯片及免疫组化EliVision法，检测35例肺鳞癌、33例肺腺癌中TRIM29、p40和TTF-1的表达以及灵敏度和特异度。结果：TRIM29、p40、TTF-1单一抗体标记，在肺鳞癌中的阳性率分别为96.97%、93.94%、6.06%，在肺腺癌中的阳性率分别为22.85%、8.57%、88.57%；联合TRIM29和p40共同标记检测在肺鳞癌中的阳性率为93.94%，在肺腺癌中共同表达的阳性率为5.71%(P＜0.001)；单一抗体检测时TRIM29和p40在肺鳞癌中的灵敏度分别是96.97%和93.94%，特异度分别是77.14%和91.43%；TTF-1在肺腺癌中的灵敏度是88.57%，特异度是93.94%；两者同时阳性的灵敏度是93.94%，特异性是94.28%。结论：TRIM29和p40两种抗体联合TTF-1检测标记肺鳞癌和肺腺癌，在鉴别诊断中具有更高的准确性。
目的：探討肌肉細胞特異性汎素蛋白連接酶29(TRIM29)、p40蛋白和甲狀腺轉錄因子-1（TTF-1）聯閤應用在肺鱗癌和肺腺癌鑒彆診斷中的價值。方法：應用組織芯片及免疫組化EliVision法，檢測35例肺鱗癌、33例肺腺癌中TRIM29、p40和TTF-1的錶達以及靈敏度和特異度。結果：TRIM29、p40、TTF-1單一抗體標記，在肺鱗癌中的暘性率分彆為96.97%、93.94%、6.06%，在肺腺癌中的暘性率分彆為22.85%、8.57%、88.57%；聯閤TRIM29和p40共同標記檢測在肺鱗癌中的暘性率為93.94%，在肺腺癌中共同錶達的暘性率為5.71%(P＜0.001)；單一抗體檢測時TRIM29和p40在肺鱗癌中的靈敏度分彆是96.97%和93.94%，特異度分彆是77.14%和91.43%；TTF-1在肺腺癌中的靈敏度是88.57%，特異度是93.94%；兩者同時暘性的靈敏度是93.94%，特異性是94.28%。結論：TRIM29和p40兩種抗體聯閤TTF-1檢測標記肺鱗癌和肺腺癌，在鑒彆診斷中具有更高的準確性。
목적：탐토기육세포특이성범소단백련접매29(TRIM29)、p40단백화갑상선전록인자-1（TTF-1）연합응용재폐린암화폐선암감별진단중적개치。방법：응용조직심편급면역조화EliVision법，검측35례폐린암、33례폐선암중TRIM29、p40화TTF-1적표체이급령민도화특이도。결과：TRIM29、p40、TTF-1단일항체표기，재폐린암중적양성솔분별위96.97%、93.94%、6.06%，재폐선암중적양성솔분별위22.85%、8.57%、88.57%；연합TRIM29화p40공동표기검측재폐린암중적양성솔위93.94%，재폐선암중공동표체적양성솔위5.71%(P＜0.001)；단일항체검측시TRIM29화p40재폐린암중적령민도분별시96.97%화93.94%，특이도분별시77.14%화91.43%；TTF-1재폐선암중적령민도시88.57%，특이도시93.94%；량자동시양성적령민도시93.94%，특이성시94.28%。결론：TRIM29화p40량충항체연합TTF-1검측표기폐린암화폐선암，재감별진단중구유경고적준학성。
objective To investigate the value of combined application of TRIM29, p40 and TTF-1 in the differential diagnosis between lung squamous cell carcinoma and lung adenocarcinoma. Methods The expres?sions of TRIM29, p40 and TTF-1 protein were detected in 35 cases of lung squamous cell carcinoma and 33 cas?es of lung adenocarcinoma using tissue microarray and immunohistochemical method. The sensitivity and speci?ficity were calculated in diagnosis. The statistical analysis was performed using SPSS 17.0 software (SPSS, Inc., Chicago, IL, USA). χ2 test was applied to compare the numerical data among groups. P<0.05 was considered to indicate a statistically significant result. Results The positive rates of TRIM29, p40 and TTF-1 protein ex?pression in lung squamous cell carcinoma were 96.97%, 93.94% and 6.06%, respectively. And the positive rates of TRIM29, p40 and TTF-1 protein expression in lung adenocarcinoma were 22.85%, 8.57% and 88.57%, re?spectively. When combined application of TRIM29 and p40 in lung cancer, the rates were 93.94% in squamous cell carcinoma and 5.71% in adenocarcinoma. Therefore the curative effect of combined application of TRIM29 and p40 in lung cancer was better than application of anyone alone(P<0.001). Applicating TRIM29 or p40 in lung squamous cell carcinoma, the sensitivity was 96.97% and 93.94%, and specificity was 77.14% and 91.43%. Applicating TTF-1 in lung adenocarcinoma, sensitivity was 93.94%, and specificity was94.28%. Conclusion Using TRIM29 or p40 combined with TTF-1 in differential diagnosis between lung squamous cell carcinoma and lung adenocarcinoma was useful in lung cancer diagnosis and clinic treatment guidance.
