重庆医学
重慶醫學
중경의학
CHONGQING MEDICAL JOURNAL
2015年
16期
2161-2163
,共3页
陈建中%杨春%刘春雷%朱房勇%胡瑜%李松
陳建中%楊春%劉春雷%硃房勇%鬍瑜%李鬆
진건중%양춘%류춘뢰%주방용%호유%리송
髁突%骨折%截骨术%超微结构
髁突%骨摺%截骨術%超微結構
과돌%골절%절골술%초미결구
condylar%fractrure%osteotomy%ultrastructure
目的:研究髁突在体内受异常应力变化后对髁突软骨超微结构的影响。方法采用4周龄SD大鼠54只建立髁突颈骨折及截骨模型,设截骨组、骨折组及空白对照组,于术后1、3、5周处死,光镜、扫描电镜(SEM )及透射电镜(T EM )观察髁突软骨结构变化。结果光镜显示术后3、5周,截骨组及骨折组髁突总厚度均小于空白对照组。SEM显示截骨组和骨折组术后5周胶原纤维暴露,截骨组手术侧还出现胶原纤维断裂、崩解。T EM 显示术后3周截骨组较空白对照组及骨折组肥大软骨细胞成熟分化增强;截骨组术后1周手术侧可见表面纤维排列紊乱甚至缺失。5周截骨组手术侧见胶原纤维排列更为紊乱,纤维层中可见到裂隙。结论体内髁突异常应力变化会持续影响髁突软骨超微结构变化,且跟应力变化的程度相关,进而引起髁突发育的异常。
目的:研究髁突在體內受異常應力變化後對髁突軟骨超微結構的影響。方法採用4週齡SD大鼠54隻建立髁突頸骨摺及截骨模型,設截骨組、骨摺組及空白對照組,于術後1、3、5週處死,光鏡、掃描電鏡(SEM )及透射電鏡(T EM )觀察髁突軟骨結構變化。結果光鏡顯示術後3、5週,截骨組及骨摺組髁突總厚度均小于空白對照組。SEM顯示截骨組和骨摺組術後5週膠原纖維暴露,截骨組手術側還齣現膠原纖維斷裂、崩解。T EM 顯示術後3週截骨組較空白對照組及骨摺組肥大軟骨細胞成熟分化增彊;截骨組術後1週手術側可見錶麵纖維排列紊亂甚至缺失。5週截骨組手術側見膠原纖維排列更為紊亂,纖維層中可見到裂隙。結論體內髁突異常應力變化會持續影響髁突軟骨超微結構變化,且跟應力變化的程度相關,進而引起髁突髮育的異常。
목적:연구과돌재체내수이상응력변화후대과돌연골초미결구적영향。방법채용4주령SD대서54지건립과돌경골절급절골모형,설절골조、골절조급공백대조조,우술후1、3、5주처사,광경、소묘전경(SEM )급투사전경(T EM )관찰과돌연골결구변화。결과광경현시술후3、5주,절골조급골절조과돌총후도균소우공백대조조。SEM현시절골조화골절조술후5주효원섬유폭로,절골조수술측환출현효원섬유단렬、붕해。T EM 현시술후3주절골조교공백대조조급골절조비대연골세포성숙분화증강;절골조술후1주수술측가견표면섬유배렬문란심지결실。5주절골조수술측견효원섬유배렬경위문란,섬유층중가견도렬극。결론체내과돌이상응력변화회지속영향과돌연골초미결구변화,차근응력변화적정도상관,진이인기과돌발육적이상。
Objective To investigate whether the abnormal stress could cause the ultrastructural change of the rats′condylar cartilage in vivo .Methods Fifty‐four SD male rats were randomly divided into condylar neck osteotomy operation(OO)group ,con‐dylar neck fracture operation(FO) group ,and control group ,with 12 rats in each group .Then the OO group rats ,FO group rats and control group rats were executed and sampled at 1st week ,3rd week and 5th week separately to observe the structure of condyle with light microscope(LM ) ,scanning electron microscope (SEM ) and transmission electron microscope (TEM ) .Results LM showed that the general condylar thickness of OO group and FO group were thinner than that of control group at 3rd and 5th week . SEM showed that the collagen and fibre were exposed in the OO group and FO group at 3rd week and 5th week ,the fibre was bro‐ken in the OO group at 5th week .TEM showed that the hypertrophic cell in OO group was more mature than those of FO group and control group at 3rd week .The arrange of fibre was disorder in OO group at 1st week ,the fibre was more disorder and there was even leak in OO group at 5th week .Conclusion The abnormal stress in vivo could result in the ultrastructural change of the rats′condyle ,and this change relates with the stess change degree and affects the growth and development of condyle .