CAJ | 학술논문

在癌症治疗过程中，大分子物质作为一种治疗剂具有很大的潜力，因其难以被有效而特异的传递到细胞内而使其治疗作用受到限制。目前发现一种叫光化学转染的技术可以有效而特异的把不同种类的分子如基因，药物，核糖体钝化蛋白，传递到胞浆中。光化学转染（Photochemical internalization PCI）是利用不同载体将大分子物质转入胞内后，光照激活分布在内吞泡上的光敏剂，产生多种种类活性氧（ROS），其中最重要的是单态氧去破坏膜的脂质双分子层发生氧化反应而使膜破裂释放内吞的物质的一种方法。
재암증치료과정중，대분자물질작위일충치료제구유흔대적잠력，인기난이피유효이특이적전체도세포내이사기치료작용수도한제。목전발현일충규광화학전염적기술가이유효이특이적파불동충류적분자여기인，약물，핵당체둔화단백，전체도포장중。광화학전염（Photochemical internalization PCI）시이용불동재체장대분자물질전입포내후，광조격활분포재내탄포상적광민제，산생다충충류활성양（ROS），기중최중요적시단태양거파배막적지질쌍분자층발생양화반응이사막파렬석방내탄적물질적일충방법。
It is widely acknowledged that macromolecules have great potential as therapeutic agent.The therapeutic usefulness of macromolecules, such as in gene therapy, is often limited by an inefifcient transfers of the macromolecules to the cytosol and lack of tissue-speciifc targeting novel technology, named photochemical internalization(PCI), was found to efifcient delivery varieties of macromolecules, such as gene, drug, ribosome-inactivatingproteins, into cytosol in a light-dependent manner. PCI is a modality for cytosolic release of macromolecules trapped in endocytic vesicles. The method is based upon photosensitizers localized in the membranes of endocytic vesicles which create membrane rupture upon light exposure by generating reactive oxygen species(ROS) Predominantly singlet oxygen.