湖北科技学院学报（医学版）
湖北科技學院學報（醫學版）
호북과기학원학보（의학판）
JOURNAL OF XIANNING UNIVERSITY (MEDICAL SCIENCES)
2015年
3期
204-206
,共3页
鲁琴艳%陈黄琴%黄彬%冯蕊
魯琴豔%陳黃琴%黃彬%馮蕊
로금염%진황금%황빈%풍예
PHBV膜%CHA%颌骨缺损
PHBV膜%CHA%頜骨缺損
PHBV막%CHA%합골결손
PHBV membrane%Coralline hydroxyapatite%Mandibular defect
目的：通过聚羟基丁酸酯－羟基戊酸酯（PHBV）膜与珊瑚羟基磷灰石（CHA）联合修复颌骨缺损，来观察和探讨骨缺损的修复方式及成骨机制，为PHBV膜将来应用于临床提供理论依据。方法实验选择54只实验动物日本大耳白兔，随机抽取48只左侧为PHBV膜与CHA复合组（实验组），右侧为单纯CHA（对照组）。在下颌骨体部双侧造成12mm ×5mm ×4mm颌骨缺损，左侧缺损处CHA填充后覆盖15mm ×10mm大小的PH－BV膜，右侧缺损处CHA填充。另外6只兔子左右两侧各造成12 mm ×5 mm ×4 mm颌骨缺损作为空白组。植入材料后在第4周、8周及12周各处死16只动物，空白组在各期处死2只，然后进行大体观察和组织学观察。结果4周实验组缺损区中央可见新生骨组织，而对照组见大量纤维结缔组织；8周实验组缺损区中央可见多处新生骨，对照组缺损区中央可见新生骨组织，但仍可见纤维结缔组织；12周实验组缺损区新生骨组织内骨细胞小，核小，对照组骨缺损区可见部分骨组织形成。结论 PHBV膜联合CHA修复颌骨缺损，比CHA单独修复颌骨缺损成骨速度更快，新形成的骨质较好，而且PHBV膜通过膜的机械性能和维持再生空间的能力，能够阻挡成纤维细胞通透，并且能在机体内维持足够长的时间以利于新骨的形成。
目的：通過聚羥基丁痠酯－羥基戊痠酯（PHBV）膜與珊瑚羥基燐灰石（CHA）聯閤脩複頜骨缺損，來觀察和探討骨缺損的脩複方式及成骨機製，為PHBV膜將來應用于臨床提供理論依據。方法實驗選擇54隻實驗動物日本大耳白兔，隨機抽取48隻左側為PHBV膜與CHA複閤組（實驗組），右側為單純CHA（對照組）。在下頜骨體部雙側造成12mm ×5mm ×4mm頜骨缺損，左側缺損處CHA填充後覆蓋15mm ×10mm大小的PH－BV膜，右側缺損處CHA填充。另外6隻兔子左右兩側各造成12 mm ×5 mm ×4 mm頜骨缺損作為空白組。植入材料後在第4週、8週及12週各處死16隻動物，空白組在各期處死2隻，然後進行大體觀察和組織學觀察。結果4週實驗組缺損區中央可見新生骨組織，而對照組見大量纖維結締組織；8週實驗組缺損區中央可見多處新生骨，對照組缺損區中央可見新生骨組織，但仍可見纖維結締組織；12週實驗組缺損區新生骨組織內骨細胞小，覈小，對照組骨缺損區可見部分骨組織形成。結論 PHBV膜聯閤CHA脩複頜骨缺損，比CHA單獨脩複頜骨缺損成骨速度更快，新形成的骨質較好，而且PHBV膜通過膜的機械性能和維持再生空間的能力，能夠阻擋成纖維細胞通透，併且能在機體內維持足夠長的時間以利于新骨的形成。
목적：통과취간기정산지－간기무산지（PHBV）막여산호간기린회석（CHA）연합수복합골결손，래관찰화탐토골결손적수복방식급성골궤제，위PHBV막장래응용우림상제공이론의거。방법실험선택54지실험동물일본대이백토，수궤추취48지좌측위PHBV막여CHA복합조（실험조），우측위단순CHA（대조조）。재하합골체부쌍측조성12mm ×5mm ×4mm합골결손，좌측결손처CHA전충후복개15mm ×10mm대소적PH－BV막，우측결손처CHA전충。령외6지토자좌우량측각조성12 mm ×5 mm ×4 mm합골결손작위공백조。식입재료후재제4주、8주급12주각처사16지동물，공백조재각기처사2지，연후진행대체관찰화조직학관찰。결과4주실험조결손구중앙가견신생골조직，이대조조견대량섬유결체조직；8주실험조결손구중앙가견다처신생골，대조조결손구중앙가견신생골조직，단잉가견섬유결체조직；12주실험조결손구신생골조직내골세포소，핵소，대조조골결손구가견부분골조직형성。결론 PHBV막연합CHA수복합골결손，비CHA단독수복합골결손성골속도경쾌，신형성적골질교호，이차PHBV막통과막적궤계성능화유지재생공간적능력，능구조당성섬유세포통투，병차능재궤체내유지족구장적시간이리우신골적형성。
Objective To investigate the effect of polyhydroxybutyrate -hydroxyvalerate ( PHBV) mem-brane combined with coralline hydroxyapatite ( CHA) on maxillary defects and its mechanism .Methods Forty-eight white healthy rabbits were divided into experimental group ( left side ) and control group ( right side ) . 12mm ×5mm ×4mm mandibular defect of bilateral mandibular corpus in the manufacture was made ,the PHBV membrane combined with CHA repaired defects in the experimental group , and only CHA repaired defects in the control group.Left and right sides of 12mm ×5mm ×4mm mandibular defects in six rabbits were used as blank group.Sixteen rabbits were sacrificed in4,8,12 weeks after operation,respectively.two in each period of control group .Results New bone could be observed in the center of defect area of experimental group after 4 weeks,but there hada large number of fibrous connective tissue in control group and new bone formation could only be seen in the bone-bed .There had many small bone cells in new bone tissue of experimental group after 12 weeks ,and some new bone cells arrange regularly as mature bone tissue .In control group ,part of bone tissue formation and the fibrous connective tissue was still visible in defect area of control group after 12 weeks.Con-clusion The combination of PHBV membrane and CHA has faster speed in bone formation of mandibular defects than that of only CHA treatment .PHBV membrane can inhibit fibrous tissue permeability and facilitate new bone formation through the mechanical property of membrane .
