CAJ | 학술논문

目的：研究进展期胃癌患者血清中血管内皮生长因子（ VEGF）及骨桥蛋白（ OPN）浓度与进展期胃癌术后复发的关系，以探讨其作为进展期胃癌术后复发筛查标记物的价值。方法采用酶联免疫吸附测定（ELISA）检测术前及术后不同时期40例进展期胃癌患者血清中VEGF、OPN浓度，观察其阳性率、表现阳性的时间与术后复发的关系，并与CEA、CA199及影像学检查相比较。结果进展期胃癌根治术后血清VEGF、OPN浓度及阳性率明显下降，与术前比较其差异有统计学意义（ P＜0．05）；术后转移或复发患者血清VEGF、OPN浓度及阳性率再次升高，与术后1月对比差异有明显统计学意义（ P＜0．01）；术后复发患者VEGF、OPN浓度及阳性率明显高于未复发者，差异有明显统计学意义（ P＜0．01）；术前及术后复发时VEGF、OPN单独检测阳性率高于CEA、CA199，其表现阳性的时间亦早于CEA、CA199，其差异均有统计学意义（ P＜0．05），联合检测时前者亦高于后者，其差异均有统计学意义（ P＜0．05）；血清VEGF、OPN浓度升高表现阳性的时间明显早于胃镜、CT等影像学检查，其差异有统计学意义（P＜0．05）。结论进展期胃癌患者血清VEGF、OPN主要由肿瘤组织分泌产生，术后监测其血清浓度可用于早期预测和发现肿瘤的转移复发。
목적：연구진전기위암환자혈청중혈관내피생장인자（ VEGF）급골교단백（ OPN）농도여진전기위암술후복발적관계，이탐토기작위진전기위암술후복발사사표기물적개치。방법채용매련면역흡부측정（ELISA）검측술전급술후불동시기40례진전기위암환자혈청중VEGF、OPN농도，관찰기양성솔、표현양성적시간여술후복발적관계，병여CEA、CA199급영상학검사상비교。결과진전기위암근치술후혈청VEGF、OPN농도급양성솔명현하강，여술전비교기차이유통계학의의（ P＜0．05）；술후전이혹복발환자혈청VEGF、OPN농도급양성솔재차승고，여술후1월대비차이유명현통계학의의（ P＜0．01）；술후복발환자VEGF、OPN농도급양성솔명현고우미복발자，차이유명현통계학의의（ P＜0．01）；술전급술후복발시VEGF、OPN단독검측양성솔고우CEA、CA199，기표현양성적시간역조우CEA、CA199，기차이균유통계학의의（ P＜0．05），연합검측시전자역고우후자，기차이균유통계학의의（ P＜0．05）；혈청VEGF、OPN농도승고표현양성적시간명현조우위경、CT등영상학검사，기차이유통계학의의（P＜0．05）。결론진전기위암환자혈청VEGF、OPN주요유종류조직분비산생，술후감측기혈청농도가용우조기예측화발현종류적전이복발。
Objective To investigate the relationship of serum levels of VEGF、OPN and recurrence of advanced gastric cancer ,and probe into the potential of VEGF and OPN as a biomarker of recurrence of ad-vanced gastric cancer .Methods 40 advanced gastric cancer patients'serum VEGF,OPN concentration were de-tected before and after operation through enzyme-linked immunosorbent assay( ELISA).Then we analyzed the relationship between positive rates and the time of positive performance occured and the recurrence after opera -tions,also compared with CEA ,CA199 and imaging examination .Results Compared with the preoperative lev-els,the concentration and positive rate of VEGF and OPN decreased obviously ,and the difference has statistical-ly significant ( P<0 .05 ) .Compared with the time one month after operation ,the concentration and positive rate of VEGF and OPN rose again , to those patients who suffer metastasis or recurrence after operation , and the difference has statistically significant (P<0.01).Compared with those patients who did not suffer metastasis or recurrence ,patients'concentration and positive rate of VEGF and OPN who suffer metastasis or recurrence after operation was much higher ,and the difference has statistically significant ( P<0 .01 ) .Before and after opera-tion when it recured ,the concentration and positive rate of VEGF and OPN was higher than CEA and CA 199 , when compared separately ,and the difference has statistically significant ( P<0 .05 ) .And the same as joint de-tection.The time when VEGF and OPN showed positive performance was earlier than gastroscope and CT per-formance,and the difference has statistically significant (P<0.05).Conclusion The content of VEGF and OPN in advanced gastric cancer patients'serum was mainly secreted by the tumor tissue ,and it's serum concen-tration monitoring after operation can be used to predict and found the transfer of tumor recurrence in an earlier time.