CAJ | 학술논문

用抗利福平标记研究变栖克雷伯菌株 SH-1在石斛苗中的定殖动态，通过温室盆栽和大棚试验，探讨其对石斛黑斑病的防治效果。结果表明：菌株能在石斛体内定殖，菌株定殖随接种时间的延长呈先增后减的变化趋势；根、茎、叶分别在接种第11、15、20天时定殖量最高，随后呈下降趋势；35 d 后分别处于4．3×104、2．32×104、1．84×104 CFU ／g 的相对稳定状态。SH-1菌剂对石斛黑斑病有明显的防治效果，相对防效达到71．53％，与常规药剂菌核净（相对防效为72％）相当。
용항리복평표기연구변서극뢰백균주 SH-1재석곡묘중적정식동태，통과온실분재화대붕시험，탐토기대석곡흑반병적방치효과。결과표명：균주능재석곡체내정식，균주정식수접충시간적연장정선증후감적변화추세；근、경、협분별재접충제11、15、20천시정식량최고，수후정하강추세；35 d 후분별처우4．3×104、2．32×104、1．84×104 CFU ／g 적상대은정상태。SH-1균제대석곡흑반병유명현적방치효과，상대방효체도71．53％，여상규약제균핵정（상대방효위72％）상당。
In this paper,made bacterial strain Klebsiella variicola SH -1 which was selected before as materi-al,explored colonization trends of Klebsiella variicola SH -1 on Dendrobium alternaria by rifampicin labeling,and then potting and greenhouse trials were conducted to discuss its control efficiency for Dendrobium alternaria.The results showed that SH -1 could colonize in Dendrobium,and the colonization trended to increase firstly and then decrease.After inoculation,colonization quantity reached the highest at 11d,15d and 20d,in roots,stems and leaves respectively,and then followed by a downward trend.Kept the relatively stable colonization level in Den-drobium,with 4.3 ×104 CFU /g,2.32 ×104 CFU /g and 1.84 ×104 CFU /g in roots,stems and leaves after 35d, respectively.The result of potted tests on prevention of the Dendrobium alternaria showed that SH -1 had obvious control effect on Dendrobium alternaria,the control effect was the same as conventional medicine Dimetachlone that the relative control effect reached 72%,which could reach 71.53%.