重庆医学
重慶醫學
중경의학
CHONGQING MEDICAL JOURNAL
2015年
18期
2474-2476
,共3页
于代华%孙绪德%柴伟%高昌俊
于代華%孫緒德%柴偉%高昌俊
우대화%손서덕%시위%고창준
红细胞生成素%脑损伤%谷氨酸转运体1%天冬氨酸氨基转移酶类
紅細胞生成素%腦損傷%穀氨痠轉運體1%天鼕氨痠氨基轉移酶類
홍세포생성소%뇌손상%곡안산전운체1%천동안산안기전이매류
erythropoietin%brain injury%glutamate transporters 1%aspartate aminotransferases
目的:探讨重组人促红细胞生成素(rh‐EPO)对大鼠急性脑损伤后脑细胞凋亡的保护作用及对GLT‐1/GLAST表达的影响。方法60只大鼠分为对照组(n=18)、急性脑损伤组(n=22)和rh‐EPO处理组(n=20),急性脑损伤组用改良Feeney′s自由落体打击器制作急性脑损伤模型,rh‐EPO处理组采用同样的方法制作脑外伤模型15 min后腹腔注射rh‐EPO ,所有动物在实验完成48 h后断头取脑。应用RT‐PCR、Western blot检测GLT‐1、GLAST mRNA及蛋白表达。结果急性脑损伤48 h后,大鼠缺血区GLT‐1和GLAST mRNA及蛋白表达较对照组均显著降低(均 P<0.01),而rh‐EPO处理组GLT‐1和GLAST 的mRNA及蛋白表达较急性脑损伤组均显著增加(均 P<0.01)。结论 EPO可显著减轻大鼠急性脑损伤,其机制可能与GLT‐1/GLAST表达上调有关。
目的:探討重組人促紅細胞生成素(rh‐EPO)對大鼠急性腦損傷後腦細胞凋亡的保護作用及對GLT‐1/GLAST錶達的影響。方法60隻大鼠分為對照組(n=18)、急性腦損傷組(n=22)和rh‐EPO處理組(n=20),急性腦損傷組用改良Feeney′s自由落體打擊器製作急性腦損傷模型,rh‐EPO處理組採用同樣的方法製作腦外傷模型15 min後腹腔註射rh‐EPO ,所有動物在實驗完成48 h後斷頭取腦。應用RT‐PCR、Western blot檢測GLT‐1、GLAST mRNA及蛋白錶達。結果急性腦損傷48 h後,大鼠缺血區GLT‐1和GLAST mRNA及蛋白錶達較對照組均顯著降低(均 P<0.01),而rh‐EPO處理組GLT‐1和GLAST 的mRNA及蛋白錶達較急性腦損傷組均顯著增加(均 P<0.01)。結論 EPO可顯著減輕大鼠急性腦損傷,其機製可能與GLT‐1/GLAST錶達上調有關。
목적:탐토중조인촉홍세포생성소(rh‐EPO)대대서급성뇌손상후뇌세포조망적보호작용급대GLT‐1/GLAST표체적영향。방법60지대서분위대조조(n=18)、급성뇌손상조(n=22)화rh‐EPO처리조(n=20),급성뇌손상조용개량Feeney′s자유락체타격기제작급성뇌손상모형,rh‐EPO처리조채용동양적방법제작뇌외상모형15 min후복강주사rh‐EPO ,소유동물재실험완성48 h후단두취뇌。응용RT‐PCR、Western blot검측GLT‐1、GLAST mRNA급단백표체。결과급성뇌손상48 h후,대서결혈구GLT‐1화GLAST mRNA급단백표체교대조조균현저강저(균 P<0.01),이rh‐EPO처리조GLT‐1화GLAST 적mRNA급단백표체교급성뇌손상조균현저증가(균 P<0.01)。결론 EPO가현저감경대서급성뇌손상,기궤제가능여GLT‐1/GLAST표체상조유관。
Objective To investigate the effect of recombinant human erythropoietin(rh‐EPO ) on acute cerebral injury and expression of GLT‐1 and GLAST in rat .Methods Sixty SD rats were randomly divided into three groups by weight :control group (n=18) ,acute cerebral injury group(n=22) and rh‐EPO conditioning group(n=20) .Acute cerebral injury models were made by modified Feeney′s method .rh‐EPO was injected in abdominal cavity 15 min after acute cerebral injury in rh‐EPO conditioning group .Rats′brain were removed 48 h after experiments .Rat GLT‐1 and GLAST mRNA expression were determined by RT‐PCR , GLT‐1 and GLAST protein expression were determined by Western blot .Results GLT‐1/GLAST mRNA and protein expression decreased significantly after acute cerebral injury(all P<0 .01) ,but increased significantly in rh‐EPO preconditioning group com‐pared with acute cerebral injury group(all P<0 .01) .Conclusion rh‐EPO preconditioning may protect against acute cerebral injury by up regulating the expression of GLT‐1/GLAST .