重庆医学
重慶醫學
중경의학
CHONGQING MEDICAL JOURNAL
2015年
18期
2471-2473
,共3页
308 nm激光%胡椒碱%黑素细胞 ,无色素%毛囊外根鞘%黑色素合成
308 nm激光%鬍椒堿%黑素細胞 ,無色素%毛囊外根鞘%黑色素閤成
308 nm격광%호초감%흑소세포 ,무색소%모낭외근초%흑색소합성
308 nm laser%piperine%melanocyte,amelanotic%outer root sheath of hair%melanogenesis
目的：研究308nm激光联合胡椒碱对毛囊外根鞘无色素黑色素细胞（AMMC）黑色素合成及TRP‐1、TRP‐2蛋白表达的影响。方法体外培养的AMMC分为联合疗法组、308nm准分子激光组、胡椒碱组、阳性对照组及空白对照组，作用24、72、120h，测定黑色素水平，激光共聚焦显微镜观察并定量分析细胞内酪氨酸酶相关蛋白酶TRP‐1、TRP‐2的表达情况。结果联合治疗组、308nm准分子激光组、胡椒碱组、阳性对照组均不同程度地促进黑色素合成，其中以联合治疗组促进作用最强。联合治疗组和胡椒碱组的0．5mmol／L胡椒碱在72h内对黑色素水平促进作用最明显。对于TRP‐1的表达，浓度为0．5mmol／L的胡椒碱即可以对其产生促进作用，但对TRP‐2生成无促进作用。308nm准分子激光对TRP‐1、TRP‐2生成均有促进作用。结论308nm准分子激光联合胡椒碱能促进AMMC细胞的黑色素合成。
目的：研究308nm激光聯閤鬍椒堿對毛囊外根鞘無色素黑色素細胞（AMMC）黑色素閤成及TRP‐1、TRP‐2蛋白錶達的影響。方法體外培養的AMMC分為聯閤療法組、308nm準分子激光組、鬍椒堿組、暘性對照組及空白對照組，作用24、72、120h，測定黑色素水平，激光共聚焦顯微鏡觀察併定量分析細胞內酪氨痠酶相關蛋白酶TRP‐1、TRP‐2的錶達情況。結果聯閤治療組、308nm準分子激光組、鬍椒堿組、暘性對照組均不同程度地促進黑色素閤成，其中以聯閤治療組促進作用最彊。聯閤治療組和鬍椒堿組的0．5mmol／L鬍椒堿在72h內對黑色素水平促進作用最明顯。對于TRP‐1的錶達，濃度為0．5mmol／L的鬍椒堿即可以對其產生促進作用，但對TRP‐2生成無促進作用。308nm準分子激光對TRP‐1、TRP‐2生成均有促進作用。結論308nm準分子激光聯閤鬍椒堿能促進AMMC細胞的黑色素閤成。
목적：연구308nm격광연합호초감대모낭외근초무색소흑색소세포（AMMC）흑색소합성급TRP‐1、TRP‐2단백표체적영향。방법체외배양적AMMC분위연합요법조、308nm준분자격광조、호초감조、양성대조조급공백대조조，작용24、72、120h，측정흑색소수평，격광공취초현미경관찰병정량분석세포내락안산매상관단백매TRP‐1、TRP‐2적표체정황。결과연합치료조、308nm준분자격광조、호초감조、양성대조조균불동정도지촉진흑색소합성，기중이연합치료조촉진작용최강。연합치료조화호초감조적0．5mmol／L호초감재72h내대흑색소수평촉진작용최명현。대우TRP‐1적표체，농도위0．5mmol／L적호초감즉가이대기산생촉진작용，단대TRP‐2생성무촉진작용。308nm준분자격광대TRP‐1、TRP‐2생성균유촉진작용。결론308nm준분자격광연합호초감능촉진AMMC세포적흑색소합성。
Objective To study the mechanism of 308 nm excimer laser combined with piperine activated the production of melanin in amelanotic melanocytes in outer root sheath of hair(AMMC) ,and promote the expression of TRP‐1、TRP‐2 protein . Methods AMMC were in incubated for 24、72、120 h in different groups(combination therapy group ,the 308 nm excimer laser group ,the piperine group ,the positive control group and the control group) .Then the melanin content was measured .The expres‐sion change of TRP‐1 and TRP‐2 in cells were observed by laser scanning confocal microscopy .Results Four groups increased the AMMC cellular melanin content .Four different groups could promote melanin content at different degrees .The combination therapy group played the strongest role in promoting function .And the function was the most obviously in 24 h ,it also was concentration dependent .0 .5 mmol/L piperine in combination group and piperine group could produce promoting effect of within 72 h on the ex‐pression of TRP‐1 ,but its role was not evident in the expression of TRP‐2 .308 nm excimer laser had effects both on TRP 1 and TRP 2 .Conclusion 308 nm excimer laser combination with piperine can increase the activation of amelanotic melanocytes in outer root sheath of hair .
