重庆医学
重慶醫學
중경의학
CHONGQING MEDICAL JOURNAL
2015年
18期
2467-2470
,共4页
吗替麦考酚酯%抗肾小球基底膜疾病%肾小球肾炎%肾功能不全%白细胞介素1
嗎替麥攷酚酯%抗腎小毬基底膜疾病%腎小毬腎炎%腎功能不全%白細胞介素1
마체맥고분지%항신소구기저막질병%신소구신염%신공능불전%백세포개소1
mycophenolate mofetil%anti-glomerular basement membrane disease%clomerulonephrits%renal insufficiency%in-terleukin-1
目的：探讨吗替麦考酚酯（MMF）对SD大鼠抗肾小球基底膜（GBM）肾炎模型的治疗作用。方法将实验动物分为空白对照组、模型对照组和M M F治疗组，利用兔抗SD大鼠GBM抗体血清制备SD大鼠抗GBM 肾炎模型。检测各组动物在给药后第0、1、2、3和4周的24 h尿蛋白、血肌酐和尿素氮等生化指标；采用酶联免疫吸附法检测在给药后第0、4周各组动物外周血中IL‐1、TGF‐β1的水平；HE染色和碘酸雪夫氏（PAS）染色分析各组动物肾脏组织病理形态学变化。结果 MMF治疗1周后，24 h尿蛋白的排泄量开始下降；MMF治疗4周后，相较于同期的模型对照组，MMF治疗组SD大鼠的24 h尿蛋白的排泄量显著性下降，外周血尿素氮、血肌酐以及与免疫相关的细胞因子IL‐1和 TGF‐β1的水平也远远低于模型对照组（P＜0．05）。 HE和PAS染色结果表明，M M F治疗组大鼠的肾小球及其周围间质区炎性细胞浸润较模型对照组减少，新月体数量明显下降。结论 MMF可提高抗GBM肾炎SD大鼠的肾功能，抑制IL‐1和TGF‐β1的产生，阻止模型大鼠的肾脏病变。
目的：探討嗎替麥攷酚酯（MMF）對SD大鼠抗腎小毬基底膜（GBM）腎炎模型的治療作用。方法將實驗動物分為空白對照組、模型對照組和M M F治療組，利用兔抗SD大鼠GBM抗體血清製備SD大鼠抗GBM 腎炎模型。檢測各組動物在給藥後第0、1、2、3和4週的24 h尿蛋白、血肌酐和尿素氮等生化指標；採用酶聯免疫吸附法檢測在給藥後第0、4週各組動物外週血中IL‐1、TGF‐β1的水平；HE染色和碘痠雪伕氏（PAS）染色分析各組動物腎髒組織病理形態學變化。結果 MMF治療1週後，24 h尿蛋白的排洩量開始下降；MMF治療4週後，相較于同期的模型對照組，MMF治療組SD大鼠的24 h尿蛋白的排洩量顯著性下降，外週血尿素氮、血肌酐以及與免疫相關的細胞因子IL‐1和 TGF‐β1的水平也遠遠低于模型對照組（P＜0．05）。 HE和PAS染色結果錶明，M M F治療組大鼠的腎小毬及其週圍間質區炎性細胞浸潤較模型對照組減少，新月體數量明顯下降。結論 MMF可提高抗GBM腎炎SD大鼠的腎功能，抑製IL‐1和TGF‐β1的產生，阻止模型大鼠的腎髒病變。
목적：탐토마체맥고분지（MMF）대SD대서항신소구기저막（GBM）신염모형적치료작용。방법장실험동물분위공백대조조、모형대조조화M M F치료조，이용토항SD대서GBM항체혈청제비SD대서항GBM 신염모형。검측각조동물재급약후제0、1、2、3화4주적24 h뇨단백、혈기항화뇨소담등생화지표；채용매련면역흡부법검측재급약후제0、4주각조동물외주혈중IL‐1、TGF‐β1적수평；HE염색화전산설부씨（PAS）염색분석각조동물신장조직병리형태학변화。결과 MMF치료1주후，24 h뇨단백적배설량개시하강；MMF치료4주후，상교우동기적모형대조조，MMF치료조SD대서적24 h뇨단백적배설량현저성하강，외주혈뇨소담、혈기항이급여면역상관적세포인자IL‐1화 TGF‐β1적수평야원원저우모형대조조（P＜0．05）。 HE화PAS염색결과표명，M M F치료조대서적신소구급기주위간질구염성세포침윤교모형대조조감소，신월체수량명현하강。결론 MMF가제고항GBM신염SD대서적신공능，억제IL‐1화TGF‐β1적산생，조지모형대서적신장병변。
Objective To investigate the influence of mycophenolate mofetil(MMF) on blood and urine biochemical indica‐tors as well as the expressions of IL‐1 in anti‐glomerular basement membrane(GBM ) nephritis SD rats .Methods The SD rats were randomly divided into control group ,model group and MMF group .And then the models of SD rat anti‐GBM nephritis were estab‐lished by injecting rabbit anti‐SD rat GBM serum antibodies .The urinary protein and blood parameters were detected initially ,1st , 2nd ,3rd and 4th weeks after administration respectively .The expressions of IL‐1 and TGF‐β1 were detected by Elisa Kit and the re‐nal pathological changes were observed by HE and PAS staining .Results After treatment for 4 weeks by MMF ,the 24 h UP ,BUN and SCr levels were lower in MMF group than those of the model group(P<0 .05) .And the expressions of IL‐1 and TGF‐β1 were decreased in MMF group .The results of HE and PAS staining showed that in model group rats ,glomerular tuft morphology was destroyed and inflammatory cell infiltration was observed in the glomeruli .Some glomeruli had cellular crescent formation .Infiltra‐tion of inflammatory cells was also observed in the interstitium .MMF clearly improved these pathological changes overall ,with im‐provements noted in both glomerular and tubular degeneration as well as in the infiltration of inflammatory cells .Conclusion MMF shows certain renal protective effect on anti‐GBM nephritis SD rats .
