CAJ | 학술논문

目的：建立生物素-链霉亲和素间接包被方法检测疑似牛奶食物不耐受患者血清特异性 IgG 抗体。方法将链霉亲和素包被反应板，牛奶的三个组分：酪蛋白、β-乳球蛋白、α-乳白蛋白标记生物素，并以3∶3∶1的比例混合，加入抗体及酶标抗人 IgG，检测绘制特异性 IgG 定量标准曲线，从而建立生物素-链霉亲和素抗原间接包被检测法检测牛奶特异性 IgG 抗体。并以美国 Biomercia 酶联免疫法作为参比方法进行方法学评价。结果本文建立的生物素-链霉亲和素间接包被法检测牛奶特异性抗体的批间精密度为7．0％-9．5％；批内精密度为4．6％-8．7％。与参比方法对比，灵敏度为92．1％，特异度为90．0％。结论本方法可用于检测牛奶特异性 IgG 抗体，用于牛奶制品引起的食物不耐受的体外诊断。
목적：건립생물소-련매친화소간접포피방법검측의사우내식물불내수환자혈청특이성 IgG 항체。방법장련매친화소포피반응판，우내적삼개조분：락단백、β-유구단백、α-유백단백표기생물소，병이3∶3∶1적비례혼합，가입항체급매표항인 IgG，검측회제특이성 IgG 정량표준곡선，종이건립생물소-련매친화소항원간접포피검측법검측우내특이성 IgG 항체。병이미국 Biomercia 매련면역법작위삼비방법진행방법학평개。결과본문건립적생물소-련매친화소간접포피법검측우내특이성항체적비간정밀도위7．0％-9．5％；비내정밀도위4．6％-8．7％。여삼비방법대비，령민도위92．1％，특이도위90．0％。결론본방법가용우검측우내특이성 IgG 항체，용우우내제품인기적식물불내수적체외진단。
Objective To establish a new method that is Biotin-Avsdin System ELISA for the concentration of food intolerance specific IgG(sIgG),taking milk sIgG detection for the example.Methods The microtiter plates were coated with streptavidin.Then,casein,β-lactoglobulin,α-lactalbumin marked biotin which mixedby 3:3:1 and specimen were succseeively added into the the wells of plate.After washing,enzyme labeled anti-human IgG was added to establish an antigen indirectly coated liquid-phase rection patterns of ELISA method.Drawing specific IgG quantitative standard de-tection curve.America Biomercia ELISA method as a reference method to evaluate.Results The method of Biotin-streptavidin indirectly coated to detect sIgG of inter batch precision is 7%-9.5%;intra assay precision ranged from 4.6% to 8.7%.Compared with the reference comparison method,the sensitivity was 92.1%,specificity was 90%.Con-clusion The detection method can be used to detect serum sIgG for milk intolerance patients.