重庆医学
重慶醫學
중경의학
CHONGQING MEDICAL JOURNAL
2015年
18期
2449-2450,2453
,共3页
李亚文%徐世元%张庆国%李乐%赖露颖%郑艇%苏娇玲%杨耐梅%李元涛
李亞文%徐世元%張慶國%李樂%賴露穎%鄭艇%囌嬌玲%楊耐梅%李元濤
리아문%서세원%장경국%리악%뢰로영%정정%소교령%양내매%리원도
SH-SY5Y细胞%活性氧%细胞凋亡%神经毒性类
SH-SY5Y細胞%活性氧%細胞凋亡%神經毒性類
SH-SY5Y세포%활성양%세포조망%신경독성류
SH-SY5Y cell%reactive Oxygen species%apoptosis%neurotoxins
目的:布比卡因作用高糖培养的SH‐SY5Y细胞,观测细胞内活性氧(ROS)及凋亡影响。方法1 mmol/L布比卡因作用不同浓度葡萄糖培养基培养的SH‐SY5Y 细胞6 h ,检测细胞内ROS、GRP78水平变化及凋亡情况。结果葡萄糖浓度为7.80、11.10、13.30 mmol/L组ROS产量均高于5.56、6.10、7.00 mmol/L组(P<0.05)。细胞凋亡率组间差异均有统计学意义(P<0.05)。GRP78表达结果为5.56 mmol/L组(1.02±0.12)、6.10 mmol/L组(0.97±0.06),7.80 mmol/L组(0.49±0.04),高于7.00 mmol/L组(0.46±0.06);11.10 mmol/L组(0.22±0.03)与13.30 mmol/L组(0.15±0.07)低于其他各组,差异均有统计学意义(P<0.05)。结论细胞内ROS增多及凋亡增加与布比卡因增强细胞内质网应激(ERS)有关。GRP78功能减弱进一步增强细胞ERS。
目的:佈比卡因作用高糖培養的SH‐SY5Y細胞,觀測細胞內活性氧(ROS)及凋亡影響。方法1 mmol/L佈比卡因作用不同濃度葡萄糖培養基培養的SH‐SY5Y 細胞6 h ,檢測細胞內ROS、GRP78水平變化及凋亡情況。結果葡萄糖濃度為7.80、11.10、13.30 mmol/L組ROS產量均高于5.56、6.10、7.00 mmol/L組(P<0.05)。細胞凋亡率組間差異均有統計學意義(P<0.05)。GRP78錶達結果為5.56 mmol/L組(1.02±0.12)、6.10 mmol/L組(0.97±0.06),7.80 mmol/L組(0.49±0.04),高于7.00 mmol/L組(0.46±0.06);11.10 mmol/L組(0.22±0.03)與13.30 mmol/L組(0.15±0.07)低于其他各組,差異均有統計學意義(P<0.05)。結論細胞內ROS增多及凋亡增加與佈比卡因增彊細胞內質網應激(ERS)有關。GRP78功能減弱進一步增彊細胞ERS。
목적:포비잡인작용고당배양적SH‐SY5Y세포,관측세포내활성양(ROS)급조망영향。방법1 mmol/L포비잡인작용불동농도포도당배양기배양적SH‐SY5Y 세포6 h ,검측세포내ROS、GRP78수평변화급조망정황。결과포도당농도위7.80、11.10、13.30 mmol/L조ROS산량균고우5.56、6.10、7.00 mmol/L조(P<0.05)。세포조망솔조간차이균유통계학의의(P<0.05)。GRP78표체결과위5.56 mmol/L조(1.02±0.12)、6.10 mmol/L조(0.97±0.06),7.80 mmol/L조(0.49±0.04),고우7.00 mmol/L조(0.46±0.06);11.10 mmol/L조(0.22±0.03)여13.30 mmol/L조(0.15±0.07)저우기타각조,차이균유통계학의의(P<0.05)。결론세포내ROS증다급조망증가여포비잡인증강세포내질망응격(ERS)유관。GRP78공능감약진일보증강세포ERS。
Objective To discuss the effect of bupivacaine on SH‐SY5Y cells in high sugar environment and observe the ROS and apoptosis .Methods 1 mmol/L bupivicaine was added to medium with different concentrations of glucose for SH‐SY5Y cells , and the quantity of ROS in the cells and the situation of apoptosis were detected by flow cytometry ,and Western blot was uesd to detect the change of the related protein ,GRP78 .Results The contents of intarcellular ROS in different groups ,which had different concentration(7 .80 ,11 .10 ,13 .30 mmol/L) of sugar medium ,were high than those in groups with concentrations 5 .56 ,6 .10 ,7 .00 mmol/L of sugar medium ,and it showed statistical significance (P<0 .05) .Cell apoptosis rates among different groups had statisti‐cal significance(P<0 .05) .Expression of GRP78 in group of 5 .56 ,6 .10 and 7 .80 mmol/L were (1 .02 ± 0 .12) ,(0 .97 ± 0 .06) and (0 .49 ± 0 .04) ,respectively ,and they all were higher than that in group of 7 .00 mmol/L (0 .46 ± 0 .06) .The groups of 11 .10 mmol/L (0 .22 ± 0 .03) and 13 .30 mmol/L (0 .15 ± 0 .07) were lower than the other groups in the expression of GRP78 ,and all the difference shows statistical significance (P<0 .05) .Conclusion Increasing and apoptosis of intracellular ROS are associated with strengthened endoplasmic reticulum stress by bupivacaine .With the function degrading of GRP78 ,endoplasmic reticulum stress (ERS) may strengthen further .