CAJ | 학술논문

目的：探讨微小染色体维持蛋白（MCM）在膀胱肿瘤组织和尿脱落细胞中的表达及临床意义。方法收集本院2012年8月—2013年8月住院患者及健康志愿者的尿脱落细胞或膀胱组织标本，对其进行 MCM5检测。结果①膀胱癌患者在不同病理分级、临床分期 MCM／β-actin 的差异有统计学意义（P ＜0．05）；②80例膀胱癌患者尿脱落细胞中有71例 MCM5呈阳性表达，与正常对照组和其他泌尿系疾病组比较，差异有统计学意义（P ＜0．05），膀胱癌患者尿脱落细胞 MCM5阳性率在各病理分级、临床分期差异无统计学意义（P 均＞0．05），但其表达在各病理分级、临床分期差异有统计学意义（P 均＜0．05）。③与尿细胞学相比，尿脱落细胞 MCM5检测诊断膀胱癌的灵敏度、阴性预测值较高，假阴性率低，差异有统计学意义（P ＜0．05），二者的特异度、假阳性率及阳性预测值比较差异无统计学意义（P ＞0．05）。结论尿脱落细胞中 MCM5检测对膀胱癌诊断意义重大，是对膀胱肿瘤复发检测、预后评价较好的指标，值得广泛应用于临床诊断。
목적：탐토미소염색체유지단백（MCM）재방광종류조직화뇨탈락세포중적표체급림상의의。방법수집본원2012년8월—2013년8월주원환자급건강지원자적뇨탈락세포혹방광조직표본，대기진행 MCM5검측。결과①방광암환자재불동병리분급、림상분기 MCM／β-actin 적차이유통계학의의（P ＜0．05）；②80례방광암환자뇨탈락세포중유71례 MCM5정양성표체，여정상대조조화기타비뇨계질병조비교，차이유통계학의의（P ＜0．05），방광암환자뇨탈락세포 MCM5양성솔재각병리분급、림상분기차이무통계학의의（P 균＞0．05），단기표체재각병리분급、림상분기차이유통계학의의（P 균＜0．05）。③여뇨세포학상비，뇨탈락세포 MCM5검측진단방광암적령민도、음성예측치교고，가음성솔저，차이유통계학의의（P ＜0．05），이자적특이도、가양성솔급양성예측치비교차이무통계학의의（P ＞0．05）。결론뇨탈락세포중 MCM5검측대방광암진단의의중대，시대방광종류복발검측、예후평개교호적지표，치득엄범응용우림상진단。
Objective To explore the expression and clinical significance of tiny chromosome maintenance protein (MCM)in bladder cancer tissue and urine exfoliated cells.Methods Urinary exfoliated cells or tissues of the bladder of hospitalized patients and healthy volunteers in our hospital from August 2012-August 2013 were collected,and the urine exfoliated cells or tumor tissue MCM5 was tested.Results ① The difference was statistically significant of clinical stagebladder cancer patients in different pathological grading MCM/β-actin (P < 0.05).② There were 71 cases with MCM5 positive in 80 bladder cancer patients with urinary exfoliated cells,differences between bladder cancer group and Normal control group or other urinary diseases group were statistically significant (P <0.05).③compared to urine cy-tology,sensitivity and negative predictive value of urine exfoliated cells MCM5 detection and diagnosis of bladder cancer was significantly higher,false-negative rate was significantly lower ,the difference was statistically significant (P <0.05),There was no statistical significance difference in specific degree,false positive rate and positive predictive value (P >0.05).Conclusion Diagnosis of MCM5 in urine exfoliated cells to detect bladder cancer have a greater sense,it is a recurrence of bladder cancer detection,prognosis better indicator,it is worth widely used in clinical diagnosis.