CAJ | 학술논문

目的：研究氯胺酮对人膀胱SV-HUC-1细胞凋亡的影响，初步探讨氯胺酮相关性膀胱功能损伤的发病机制。方法：培养人膀胱SV-HUC-1永生化上皮细胞系，用不同质量浓度的氯胺酮作用于SV-HUC-1细胞，作用不同时间后用Annexin V-FITC/PI双染法流式细胞术检测细胞凋亡率，用Western印迹法检测Bax，Bcl-2， pro-caspase-3，cleaved caspase-3蛋白表达的含量。结果：与对照组相比，加入氯胺酮后SV-HUC-1细胞凋亡率增加， Bax表达增高，Bcl-2表达减弱，Bax与Bcl-2比值增高，pro-caspase-3表达减少，cleaved caspase-3表达增高(P<0.05)，且与氯胺酮质量浓度和作用时间相关(P<0.05)。结论：氯胺酮能够明显促进人膀胱SV-HUC-1细胞的凋亡，并呈浓度依赖性和时间依赖性。
목적：연구록알동대인방광SV-HUC-1세포조망적영향，초보탐토록알동상관성방광공능손상적발병궤제。방법：배양인방광SV-HUC-1영생화상피세포계，용불동질량농도적록알동작용우SV-HUC-1세포，작용불동시간후용Annexin V-FITC/PI쌍염법류식세포술검측세포조망솔，용Western인적법검측Bax，Bcl-2， pro-caspase-3，cleaved caspase-3단백표체적함량。결과：여대조조상비，가입록알동후SV-HUC-1세포조망솔증가， Bax표체증고，Bcl-2표체감약，Bax여Bcl-2비치증고，pro-caspase-3표체감소，cleaved caspase-3표체증고(P<0.05)，차여록알동질량농도화작용시간상관(P<0.05)。결론：록알동능구명현촉진인방광SV-HUC-1세포적조망，병정농도의뢰성화시간의뢰성。
Objective:To determine the effect of ketamine on the apoptosis of human uroepithelial cells (SV-HUC-1) and the pathogenesis of ketamine-associated cystitis. Methods:SV-HUC-1 cells were cultured under various concentrations of ketamine and different time. Flow cytometry was used to analyze the rate of cell apoptosis. hTe protein levels of Bax, Bcl-2, pro-caspase-3, and cleaved caspase-3 were detected by Western blot. Results:Compared with the control group, the apoptotic rate of ketamine cultured SV-HUC-1 cells increased. hTe expression of Bax increased, Bcl-2 expression decreased, and Bax/Bcl-2 in the ketamine cultured SV-HUC-1 cells was signiifcantly higher. hTe protein level of pro-caspase-3 was signiifcantly lower, and that of cleaved caspase-3 was signiifcantly higher than that in the control group (P<0.05), positively correlated with the dose of ketamine and time of culture (P<0.05). Conclusion:Ketamine can induce the apoptosis of SV-HUC-1 cells in a dose and time dependent manner.