CAJ | 학술논문

目的：观察幼龄大鼠和老龄大鼠脑组织内不同部位VEGF的表达及微血管密度(MVD)，探讨其在神经系统衰老过程中的作用。方法：应用免疫组织化学对比观察3月龄组和30月龄组大鼠脑组织不同部位VEGF的表达及MVD。结果：30月龄组大鼠运动皮质区、海马CA1区、海马CA3区及第1小脑小叶区VEGF阳性细胞个数及MVD较3月龄组对应区域明显减少，差异有统计学意义(P<0.01)；同组大鼠不同部位阳性细胞个数均有明显变化(P<0.01)，其中运动皮质区表达最强，其次是海马CA3区、海马CA1区及第1小脑小叶区。结论：老龄大鼠脑组织各部位VEGF及MVD较幼龄大鼠明显减少，且不同部位VEGF的表达及MVD差异明显，其中运动皮质区表达最强，提示补充外源性VEGF，可能可以促进脑组织内微血管的生成，延缓神经系统衰老。
목적：관찰유령대서화노령대서뇌조직내불동부위VEGF적표체급미혈관밀도(MVD)，탐토기재신경계통쇠로과정중적작용。방법：응용면역조직화학대비관찰3월령조화30월령조대서뇌조직불동부위VEGF적표체급MVD。결과：30월령조대서운동피질구、해마CA1구、해마CA3구급제1소뇌소협구VEGF양성세포개수급MVD교3월령조대응구역명현감소，차이유통계학의의(P<0.01)；동조대서불동부위양성세포개수균유명현변화(P<0.01)，기중운동피질구표체최강，기차시해마CA3구、해마CA1구급제1소뇌소협구。결론：노령대서뇌조직각부위VEGF급MVD교유령대서명현감소，차불동부위VEGF적표체급MVD차이명현，기중운동피질구표체최강，제시보충외원성VEGF，가능가이촉진뇌조직내미혈관적생성，연완신경계통쇠로。
Objective:To observe the distribution of vascular endothelial growth factor (VEGF) and microvessel density (MVD) in different brain regions in aged rats and determine the role of VEGF and MVD in the aging process of the nervous system. Methods:We observed the expression of VEGF and MVD in different parts of rat brain in the 3- month group and 30-month group with immunohistochemical technique. Results:Compared with the 3-month group, the 30-month group showed fewer VEGF-positive cells and MVD in the brain (P<0.01), and the number varied signiifcantly in different brain regions(P<0.01). The motor cortex region contained more VEGF-positive cells and MVD than the hippocampus and cerebellum. Conclusion:VEGF-positive cells and MVD are decreased in every brain region of aged rats, and the motor cortex region contains more positive cells, suggesting exogenous VEGF may enhance the formation of microvessels and delay the aging of the nervous system.