CAJ | 학술논문

目的：探讨姜黄素在慢性低灌注大鼠的神经功能保护作用。方法：制备慢性脑缺血模型(2-VO)模型，术后分别给予姜黄素(实验组)和磷酸盐缓冲液(PBS，对照组)干预。术后8周后行Morris水迷宫评价大鼠的空间学习记忆功能变化。同时从Nissl染色观察大鼠神经细胞的变化；Western blot检测炎症因子IL-1β、TNF-α的表达。结果：实验组大鼠的空间学习记忆能力均较对照组明显改善，差异有统计学意义(P<0.05)；Nissl染色发现实验组大鼠的缺血区神经元细胞与形态相对较为完整，而对照组大鼠神经元形态不完整，仅有散在的尼氏小体；在姜黄素治疗后缺血区脑细胞中IL-1β、TNF-α的含量明显降低(P<0.05)。结论：姜黄素可以显著改善慢性脑缺血大鼠的空间学习记忆能力，可能与其抗炎症反应相关。
목적：탐토강황소재만성저관주대서적신경공능보호작용。방법：제비만성뇌결혈모형(2-VO)모형，술후분별급여강황소(실험조)화린산염완충액(PBS，대조조)간예。술후8주후행Morris수미궁평개대서적공간학습기억공능변화。동시종Nissl염색관찰대서신경세포적변화；Western blot검측염증인자IL-1β、TNF-α적표체。결과：실험조대서적공간학습기억능력균교대조조명현개선，차이유통계학의의(P<0.05)；Nissl염색발현실험조대서적결혈구신경원세포여형태상대교위완정，이대조조대서신경원형태불완정，부유산재적니씨소체；재강황소치료후결혈구뇌세포중IL-1β、TNF-α적함량명현강저(P<0.05)。결론：강황소가이현저개선만성뇌결혈대서적공간학습기억능력，가능여기항염증반응상관。
Objective: Explore the effect of curcunmin on chronic cerebral ischemia in rats, and its possible mechanism. Methods: Chronic cerebral ischemia (2-VO) model were prepared and given curcunmin or PBS atfer the surge. Eight weeks atfer the operation, Morris water maze were carried out to evaluate the learning and memory ability of the rats. hTe change of nerve cells were observed by Nissl staining; the expression of IL-1β,TNF-α were detected by western blot.Results: hTe learning and memory ability of the curcunmin group were signiifcantly improved compared with the control group. hTe neurons pattern of curcumin treatment group was similar to the control group, while only scattered Nissl bodies were found the curcumin group; the expression of IL-1β,TNF-α in curcumin treatment group were signiifcantly reduced (P<0.05).Conclusion: Curcumin can signiifcantly improve the learning and memory ability of the chronic cerebral ischemic rat and its possible mechanism maybe involve in the anti-inlfammation in neurons.