CAJ | 학술논문

使用扫描电子显微镜（SEM）、红外图谱（IR）、循环伏安（CV）、差示脉冲循环伏安（DPV）对血红蛋白聚合物膜修饰电极进行了表征。 DPV测试表明血红蛋白的浓度和峰电流分别在低浓度和高浓度下均有良好的线性关系，低浓度下满足线性回归方程ip（μA）＝13.05－3.58C（μM），相关系数R为－0.9960。检出限为2.7×10－9 M，线性范围8.0×10－9～7.1×10－7 M；高浓度下满足线性回归方程Ip（μA）＝10.56－0.1001C（μM），相关系数R为－0.9957。检出限为5.7×10－7 M，线性范围1.7×10－6～1.4×10－5 M。可用于构建生物分子印迹的电化学传感器。
사용소묘전자현미경（SEM）、홍외도보（IR）、순배복안（CV）、차시맥충순배복안（DPV）대혈홍단백취합물막수식전겁진행료표정。 DPV측시표명혈홍단백적농도화봉전류분별재저농도화고농도하균유량호적선성관계，저농도하만족선성회귀방정ip（μA）＝13.05－3.58C（μM），상관계수R위－0.9960。검출한위2.7×10－9 M，선성범위8.0×10－9～7.1×10－7 M；고농도하만족선성회귀방정Ip（μA）＝10.56－0.1001C（μM），상관계수R위－0.9957。검출한위5.7×10－7 M，선성범위1.7×10－6～1.4×10－5 M。가용우구건생물분자인적적전화학전감기。
The structure and properties of hemoglobin modified electrode ( BHb/MIP-CPE ) were characterized by scanning electron microscopy ( SEM ) , IR spectrum , cyclic voltammograms ( CV ) , differential pulse voltammograms ( DPV).The sensor was tested by differential pulse voltammetry ( DPV ) to investigate the relationship between the response current and concentration.The results showed that a wide detection linear range of 8.0 ×10-9 ~7.1 ×10-7 M and 1.7 ×10-6 ~1.4 ×10-5 M for the determination of hemoglobin in low concentration and high concentration with the low detection limit of 2.7 ×10-9 M ( S/N=3 ) and 5.7 ×10-7 M ( S/N=3 ) were obtained , respectively.Paper successfully constructed electrochemical sensor based on biological molecular imprinting.