CAJ | 학술논문

以高丛康维尔蓝莓组培苗为材料，用不同质量浓度的秋水仙素通过浸渍法和培养法进行染色体加倍研究，从形态学、生理生化学、解剖学及细胞学进行比较，以确定能够诱导出多倍体的最佳处理方法和处理质量浓度．结果发现，在形态指标上，0．5，2．0，4．0 mg／mL 秋水仙素浸渍处理1 d 及0．4 mg／mL 秋水仙素培养10 d 后，蓝莓的叶长、叶宽、茎直径、叶厚、茎直径的平均值均显著大于 CK；在解剖结构上，0．5 mg／mL 浸渍1 d 和0．4 mg／mL 培养10 d 后，其气孔变大，密度变低，为椭圆形，保卫细胞的淀粉粒减少，0．5，2．0 mg／mL秋水仙素浸渍1 d 和0．4 mg／mL 培养10 d 后均使细胞核明显变大；在生理指标上，0．5 mg／mL 浸渍处理1 d后显著提高了蓝莓 SOD 酶活性，2．0，4．0 mg／mL 秋水仙素浸渍处理1 d 能够显著提高可溶性蛋白质含量，其中后者还显著提高了其叶绿素 A 含量．可见，0．5 mg／mL 浸渍1 d 和0．4 mg／mL 培养10 d 后，蓝莓植株发生多倍性变异的可能性最大，但还需后续的大田鉴定，才能最终确定多倍体植株．
이고총강유이람매조배묘위재료，용불동질량농도적추수선소통과침지법화배양법진행염색체가배연구，종형태학、생리생화학、해부학급세포학진행비교，이학정능구유도출다배체적최가처리방법화처리질량농도．결과발현，재형태지표상，0．5，2．0，4．0 mg／mL 추수선소침지처리1 d 급0．4 mg／mL 추수선소배양10 d 후，람매적협장、협관、경직경、협후、경직경적평균치균현저대우 CK；재해부결구상，0．5 mg／mL 침지1 d 화0．4 mg／mL 배양10 d 후，기기공변대，밀도변저，위타원형，보위세포적정분립감소，0．5，2．0 mg／mL추수선소침지1 d 화0．4 mg／mL 배양10 d 후균사세포핵명현변대；재생리지표상，0．5 mg／mL 침지처리1 d후현저제고료람매 SOD 매활성，2．0，4．0 mg／mL 추수선소침지처리1 d 능구현저제고가용성단백질함량，기중후자환현저제고료기협록소 A 함량．가견，0．5 mg／mL 침지1 d 화0．4 mg／mL 배양10 d 후，람매식주발생다배성변이적가능성최대，단환수후속적대전감정，재능최종학정다배체식주．
Taking High-bush Convair blueberry plantlets as materials,through impregnation and culture with colchicine of different concentrations,chromosome doubling was studied.From the morphology,physiology and biochemistry,anatomy and cytology were compared in order to determine the best treatment method and treatment quality concentration to induce polyploidy.The results showed that,in morphology and anatomy,the mean of blueberry the leaf length,leaf width,stem diameter and leaf thickness were significantly greater than CK after 0.5,2.0 and 4.0 mg/mL colchicine soaking for 1 d and 0.4 mg/mL colchicine culture for 10 d;in anatomical structure,0.5 mg/mL impregnated for 1 d and 0.4 mg/mL culture for 10 d,the stomatal density of blueberry becomes larger,low, oval,starch grains in guard cells reduced,and the nucleus becomes larger after 0.5,2.0 mg/mL soaking for 1 d and 0.4 mg/mL colchicine impregnated culture for 10 d were significantly larger nuclear;in the physiological indexes,0.5 mg/mL impregnated for 1 d significantly increased SOD activity of blueberry,colchicine soaking for 1 d and 4.0 mg/mL could significantly improve soluble protein content,the latter also significantly increased chlorophyll a content.Thus,after 10 d culture 0.5 mg/mL impregnated for 1 d and 0.4 mg/mL,the possibility of the occurrence of the blueberry plant polyploidy variant of the maximum,but need field identification of follow-up to ultimately determine the polyploid plants.