CAJ | 학술논문

用酸提取法从鲫鱼中提取胶原蛋白，用不同浓度的盐酸作为脱钙溶液，以0.5 mol／L乙酸—乙酸钠缓冲溶液提取其胶原蛋白。单因素分析与正交试验结果表明：提取温度为6～10℃时，最佳提取条件为固液比1砄20，提取时间每次1.5 h，脱钙酸浓度为0.5 mol／L，脱钙时间3 h，胶原蛋白提取率为0.773％。
용산제취법종즉어중제취효원단백，용불동농도적염산작위탈개용액，이0.5 mol／L을산—을산납완충용액제취기효원단백。단인소분석여정교시험결과표명：제취온도위6～10℃시，최가제취조건위고액비1결20，제취시간매차1.5 h，탈개산농도위0.5 mol／L，탈개시간3 h，효원단백제취솔위0.773％。
Using hydrochloric acid with different concentrations as decalcification solution and the mixture of acetic and sodium acetate as buffer solution , the collagen wan extracted from erucian fish scale.The univariate analysis and orthogonal test results showed that the extraction ratio was 0.773% under the optimal conditions: the ratio of solid to solvent as 1:20, the extracting time as 1.5 days, the concentration of hydrochloric acid for decalcification as 0.5 mol/L and the decalcification times as 3 h.