中华内分泌代谢杂志
中華內分泌代謝雜誌
중화내분비대사잡지
CHINESE JOURNAL OF ENDOCRINOLOGY AND METABOLISM
2015年
5期
427-433
,共7页
杨敏%都昌吉%王引萍%刘军
楊敏%都昌吉%王引萍%劉軍
양민%도창길%왕인평%류군
桥本甲状腺炎%调节性B淋巴细胞%抑制性T细胞
橋本甲狀腺炎%調節性B淋巴細胞%抑製性T細胞
교본갑상선염%조절성B림파세포%억제성T세포
Hashimotos' thyroiditis%Regulatory B lymphocyte cells%Suppressor T lymphocyte cell
目的 探讨桥本甲状腺炎(HT)患者外周血调节性B淋巴细胞(regulatory B cell,Bregs)、抑制性T细胞(suppressorT cell,Ts)的比例变化.方法 入组18 ～ 60岁桥本甲状腺炎患者61例,性别年龄相匹配的健康组38名.应用流式细胞技术检测外周血CD19+ CD24+ CD27+B细胞、CD19+ CD24hi CD38hiB细胞、CD19+白细胞介素-10+B细胞及CD8+ CD28-T细胞比例变化.结果 与对照组相比,HT组CD19+CD24+CD27+B细胞亚群显著升高(P＜0.05).随着甲状腺过氧化物酶抗体的升高,CD19+ CD24hi CD38hiB细胞显著降低(P＜0.05);随着甲状腺球蛋白抗体(TGAb)的升高CD8+ CD28-T细胞显著降低(P＜0.05).三碘甲状腺原氨酸(TT3)与CD19+ CD24hiCD38hiB细胞则呈显著正相关;甲状腺素(TT4)与CD8+CD28 T细胞呈显著负相关,与CD19+CD24+CD27+B细胞则呈显著正相关(P＜0.05).Logistic回归分析发现,TGAb和游离甲状腺素(FT4)是CD8+ CD28-T细胞在淋巴细胞中比例降低的独立危险因素(P＜0.05);促甲状腺素(TSH)是CD19+ CD24hiCD38hiB细胞比例降低的独立危险因素,FT4是CD19+CD24+CD27+B细胞比例升高的独立危险因素(P＜0.05).结论 HT患者外周血Ts显著降低,不同表型的Bregs比例变化则不同,甲状腺自身抗体和甲状腺功能均与HT的免疫改变相关.
目的 探討橋本甲狀腺炎(HT)患者外週血調節性B淋巴細胞(regulatory B cell,Bregs)、抑製性T細胞(suppressorT cell,Ts)的比例變化.方法 入組18 ～ 60歲橋本甲狀腺炎患者61例,性彆年齡相匹配的健康組38名.應用流式細胞技術檢測外週血CD19+ CD24+ CD27+B細胞、CD19+ CD24hi CD38hiB細胞、CD19+白細胞介素-10+B細胞及CD8+ CD28-T細胞比例變化.結果 與對照組相比,HT組CD19+CD24+CD27+B細胞亞群顯著升高(P＜0.05).隨著甲狀腺過氧化物酶抗體的升高,CD19+ CD24hi CD38hiB細胞顯著降低(P＜0.05);隨著甲狀腺毬蛋白抗體(TGAb)的升高CD8+ CD28-T細胞顯著降低(P＜0.05).三碘甲狀腺原氨痠(TT3)與CD19+ CD24hiCD38hiB細胞則呈顯著正相關;甲狀腺素(TT4)與CD8+CD28 T細胞呈顯著負相關,與CD19+CD24+CD27+B細胞則呈顯著正相關(P＜0.05).Logistic迴歸分析髮現,TGAb和遊離甲狀腺素(FT4)是CD8+ CD28-T細胞在淋巴細胞中比例降低的獨立危險因素(P＜0.05);促甲狀腺素(TSH)是CD19+ CD24hiCD38hiB細胞比例降低的獨立危險因素,FT4是CD19+CD24+CD27+B細胞比例升高的獨立危險因素(P＜0.05).結論 HT患者外週血Ts顯著降低,不同錶型的Bregs比例變化則不同,甲狀腺自身抗體和甲狀腺功能均與HT的免疫改變相關.
목적 탐토교본갑상선염(HT)환자외주혈조절성B림파세포(regulatory B cell,Bregs)、억제성T세포(suppressorT cell,Ts)적비례변화.방법 입조18 ～ 60세교본갑상선염환자61례,성별년령상필배적건강조38명.응용류식세포기술검측외주혈CD19+ CD24+ CD27+B세포、CD19+ CD24hi CD38hiB세포、CD19+백세포개소-10+B세포급CD8+ CD28-T세포비례변화.결과 여대조조상비,HT조CD19+CD24+CD27+B세포아군현저승고(P＜0.05).수착갑상선과양화물매항체적승고,CD19+ CD24hi CD38hiB세포현저강저(P＜0.05);수착갑상선구단백항체(TGAb)적승고CD8+ CD28-T세포현저강저(P＜0.05).삼전갑상선원안산(TT3)여CD19+ CD24hiCD38hiB세포칙정현저정상관;갑상선소(TT4)여CD8+CD28 T세포정현저부상관,여CD19+CD24+CD27+B세포칙정현저정상관(P＜0.05).Logistic회귀분석발현,TGAb화유리갑상선소(FT4)시CD8+ CD28-T세포재림파세포중비례강저적독립위험인소(P＜0.05);촉갑상선소(TSH)시CD19+ CD24hiCD38hiB세포비례강저적독립위험인소,FT4시CD19+CD24+CD27+B세포비례승고적독립위험인소(P＜0.05).결론 HT환자외주혈Ts현저강저,불동표형적Bregs비례변화칙불동,갑상선자신항체화갑상선공능균여HT적면역개변상관.
Objective To explore the proportional changes of regulatory B lymphocyte cells (Bregs) and suppressor T lymphocyte cell (Ts) in the peripheral blood of patients with Hashimotos' thyroiditis (HT).Method Sixty-one cases of HT and 38 healthy subjects with matched age and gender were recruited.Flow cytometry technology was used to detect the proportional changes of CD19+ CD24+ CD27 + B cells,CD19+ CD24hi CD38hi B cells,CD19+ IL-10+B cells,and CD8+CD28-T cells.Result Compared with control group,CD19+CD24+CD27+B cells in HT group increased significantly(P＜0.05).With the increase of thyroid peroxidase antibody,CD19+ CD24hi CD38hi B cells were decreased significantly.With the increase of antithyrogloblin antibody (TGAb),CD8 + CD28-T cells were decreased markedly (P＜0.05).TT3 was positively related with CD19 + CD24hi CD38 hiB cells while TT4 had a negative correlation with CD8+ CD28-T cells and positive correlation with CD19+ CD24+ CD27+ B cells.Logistic regression analysis showed that TGAb and FT4 were independent risk factors for decreased CD8+ CD28-T cells in lymphocyte percentage(P＜0.05).TSH was an independent risk factor for the decreased proportion of CD19+ CD24hi CD38hiB cell.FT4 was the independent risk factors for increased CD19+CD24+CD27+B cells(P＜0.05).Conclusion The percentage of Ts cells in patients with HT was lowered,while the different phenotypic Bregs cells showed various changes.Thyroid autoantibodies and thyroid function were closely associated with the immunological changes in HT patients.
