昆明学院学报
昆明學院學報
곤명학원학보
JOURNAL OF KUNMING UNIVERSITY
2015年
3期
56-60
,共5页
浊点萃取%AEO -9%滇重楼%偏诺皂苷
濁點萃取%AEO -9%滇重樓%偏諾皂苷
탁점췌취%AEO -9%전중루%편낙조감
cloud point extraction%AEO -9%Paris polyphylla Sm.%Pennogenin
建立采用涡旋辅助浊点萃取滇重楼提取物中4个偏诺皂苷类成分，以非离子表面活性剂脂肪醇聚氧乙烯醚 AEO －9为萃取剂，V（乙腈）∶V（水）＝38∶62为流动相，对富集的偏诺皂苷进行高效液相色谱快速分离的方法．最优实验条件为，AEO －9用量为110μL，正戊酸用量为150μL，pH 为9，平衡温度为40℃，平衡时间为15 min．结果表明，检测出滇重楼中4个偏诺皂苷 VI，VII，Pa 及 H 的质量分数分别为0．9923％，1．3603％，3．3444％及1．6663％，检测限达3．2～4．0μg／L，RSD 低于0．5％，定量限为6．6～8．3μg／L，线性范围为10～500μg／L，萃取的富集倍数达到23倍，萃取的回收率为98．68％～102．46％．
建立採用渦鏇輔助濁點萃取滇重樓提取物中4箇偏諾皂苷類成分，以非離子錶麵活性劑脂肪醇聚氧乙烯醚 AEO －9為萃取劑，V（乙腈）∶V（水）＝38∶62為流動相，對富集的偏諾皂苷進行高效液相色譜快速分離的方法．最優實驗條件為，AEO －9用量為110μL，正戊痠用量為150μL，pH 為9，平衡溫度為40℃，平衡時間為15 min．結果錶明，檢測齣滇重樓中4箇偏諾皂苷 VI，VII，Pa 及 H 的質量分數分彆為0．9923％，1．3603％，3．3444％及1．6663％，檢測限達3．2～4．0μg／L，RSD 低于0．5％，定量限為6．6～8．3μg／L，線性範圍為10～500μg／L，萃取的富集倍數達到23倍，萃取的迴收率為98．68％～102．46％．
건립채용와선보조탁점췌취전중루제취물중4개편낙조감류성분，이비리자표면활성제지방순취양을희미 AEO －9위췌취제，V（을정）∶V（수）＝38∶62위류동상，대부집적편낙조감진행고효액상색보쾌속분리적방법．최우실험조건위，AEO －9용량위110μL，정무산용량위150μL，pH 위9，평형온도위40℃，평형시간위15 min．결과표명，검측출전중루중4개편낙조감 VI，VII，Pa 급 H 적질량분수분별위0．9923％，1．3603％，3．3444％급1．6663％，검측한체3．2～4．0μg／L，RSD 저우0．5％，정량한위6．6～8．3μg／L，선성범위위10～500μg／L，췌취적부집배수체도23배，췌취적회수솔위98．68％～102．46％．
To establish a method using vortex-assisted cloud point extraction for four pennogenin Ingredients of Paris polyphylla Sm., taking nonionic surfactant primary alcobol ethoxylate (AEO -9)as the extractant,using a mixture of acetonitrile-water (38 ∶62,by volume)as the mobile phase,the enrichment Pennogenin Ingredients was rapid performed by high performance liquid chromatography (HPLC).Under the optimized conditions,the volume of AEO -9 was 110 μL,the volume of n-Valerate was 150 μL,pH was 9.0,the equilibration time and temperature were 40 ℃ and 15 min,respectively.The result shows that the contents of four Pennogenin Ingredients of Paris polyphylla Sm.VI,VII,Pa and H were 0.992 3%,1.360 3%,3.344 4% and 1.666 3%,respectively.The detection of limits (LOD)was 3.2—4.0 μg/L;the relative standard deviations (RSD)was below 0.5% (n =9 );the limit of quantification (LOQ)was 6.6—8.3 μg/L;the linear range was 10—500 μg/L;the enrichment factor was 23 and the relative recoveries was ranged from 98.68% to 102.46%.
