CAJ | 학술논문

以1、10、20 μmol/L格列苯脲处理MC3T3-E1细胞48 h后,利用CCK-8比色法检测细胞的增殖率,流式细胞术检测凋亡率,实时荧光定量PCR检测Ⅰ型胶原蛋白(COL-1)、骨桥蛋白(OPN)的表达.Western印迹法检测凋亡相关蛋白Bax、Bcl-2的表达.结果显示与对照组相比,随着格列苯脲浓度的升高,MC3T3-E1细胞的增殖率逐渐上升(P＜0.05),凋亡率逐渐下降(P＜0.05),COL-1、OPN mRNA和Bcl-2蛋白表达逐步升高(均P＜0.05),Bax蛋白的表达逐步下降(P＜0.05).提示格列苯脲能够在高糖环境下促进MC3T3-E1细胞的增殖和分化,并抑制其凋亡,且在一定范围内具有浓度依赖性.
이1、10、20 μmol/L격렬분뇨처리MC3T3-E1세포48 h후,이용CCK-8비색법검측세포적증식솔,류식세포술검측조망솔,실시형광정량PCR검측Ⅰ형효원단백(COL-1)、골교단백(OPN)적표체.Western인적법검측조망상관단백Bax、Bcl-2적표체.결과현시여대조조상비,수착격렬분뇨농도적승고,MC3T3-E1세포적증식솔축점상승(P＜0.05),조망솔축점하강(P＜0.05),COL-1、OPN mRNA화Bcl-2단백표체축보승고(균P＜0.05),Bax단백적표체축보하강(P＜0.05).제시격렬분뇨능구재고당배경하촉진MC3T3-E1세포적증식화분화,병억제기조망,차재일정범위내구유농도의뢰성.
After MC3T3-E1 cells were treated with 1,10,and 20 μmol/L glibenclamide for 48 h,the proliferation rate of cells was detected by CCK-8 assay.Flow cytometry was used to test cell apoptosis.The mRNA expressions of collagen I (COL-1) and osteopontin (OPN) were tested by realtime fluorescence quantitative PCR.Western blot was used to detect the expression levels of apoptosis-related proteins Bax and Bcl-2.The results showed that compared with the control group,the proliferation rate of MC3T3-E1 cells was gradually increased (P＜0.05),the apoptosis rate decreased (P ＜ 0.05),the expressions of COL-1 mRNA,OPN mRNA,and Bcl-2 protein were progressively raised (P＜0.05),and the expression of Bax protein were gradually decreased (P＜0.05) along with increasing concentration of glyburide.It suggested that glibenclamide could promote the proliferation and differentiation of MC3T3-E1 cells in high glucose and may inhibit apoptosis in a concentration-dependent manner within a certain range.