广州化工
廣州化工
엄주화공
GUANGZHOU CHEMICAL INDUSTRY AND TECHNOLOGY
2015年
12期
104-105,155
,共3页
许继承%高明波%王婷%张钰昆%毕丽君%潘慧
許繼承%高明波%王婷%張鈺昆%畢麗君%潘慧
허계승%고명파%왕정%장옥곤%필려군%반혜
地黄%生地%熟地%梓醇%HPLC
地黃%生地%熟地%梓醇%HPLC
지황%생지%숙지%재순%HPLC
Radix Rehmanniae%raw radix rehmanniae%rehmannia glutinosa%catalpol%HPLC
中药地黄中主要的化学成分是环烯醚萜类,其中梓醇含量较高,是地黄中主要活性成分,具有抗癌、利尿、缓泻、降血糖等作用。传统用药部位是其新鲜或干燥块根。本实验选取制药时废弃不用的地黄叶子为原材料,经实验确定以95%乙醇为提取溶剂,超声20 min提取梓醇。并同时从新鲜地黄叶、干地黄叶、生地、熟地中提取梓醇,采用HPLC分别测得提取物中梓醇的含量。结果表明,鲜地黄叶,干地黄叶,生地和熟地中的梓醇含量分别为1.07,9.93,14.24,12.54 mg/g。从地黄叶片中提取梓醇具有一定的可行性。
中藥地黃中主要的化學成分是環烯醚萜類,其中梓醇含量較高,是地黃中主要活性成分,具有抗癌、利尿、緩瀉、降血糖等作用。傳統用藥部位是其新鮮或榦燥塊根。本實驗選取製藥時廢棄不用的地黃葉子為原材料,經實驗確定以95%乙醇為提取溶劑,超聲20 min提取梓醇。併同時從新鮮地黃葉、榦地黃葉、生地、熟地中提取梓醇,採用HPLC分彆測得提取物中梓醇的含量。結果錶明,鮮地黃葉,榦地黃葉,生地和熟地中的梓醇含量分彆為1.07,9.93,14.24,12.54 mg/g。從地黃葉片中提取梓醇具有一定的可行性。
중약지황중주요적화학성분시배희미첩류,기중재순함량교고,시지황중주요활성성분,구유항암、이뇨、완사、강혈당등작용。전통용약부위시기신선혹간조괴근。본실험선취제약시폐기불용적지황협자위원재료,경실험학정이95%을순위제취용제,초성20 min제취재순。병동시종신선지황협、간지황협、생지、숙지중제취재순,채용HPLC분별측득제취물중재순적함량。결과표명,선지황협,간지황협,생지화숙지중적재순함량분별위1.07,9.93,14.24,12.54 mg/g。종지황협편중제취재순구유일정적가행성。
The main chemical component of Traditional Chinese medicine Radix Rehmanniae is iridoids.Among which the catalpol content is higher , being a major active ingredient in Radix Rehmanniae with activities of anti-cancer , diuresis, laxness, antihypertension and so on.The traditonal application sites are the fresh or dry roots.The leaves of Radix Rehmanniae that were abandoned in phamary used as materials.Catalpol was ultrasonic extracted with 95%ethanol for 20 min.Catalpol was ultrasonic extracted simutaneously from fresh and dry leaves of Radix Rehmanniae, raw radix rehmanniae and rehmannia glutinosa and determined by HPLC.The results showed that the catalpol content of fresh and dry leaves of Radix Rehmanniae, raw radix rehmanniae and rehmannia glutinosa were 1.07 , 9.93 , 14.24 , 12.54 mg/g respectively.It's feasible to extract catalpol from the leaves of Radix Rehmanniae.