实用心脑肺血管病杂志
實用心腦肺血管病雜誌
실용심뇌폐혈관병잡지
PRACTICAL JOURNAL OF CARDIAC CEREBRAL PNEUMAL AND VASCULAR DISEASE
2015年
5期
31-35
,共5页
符代炎%戴爱国%胡瑞成%田华%陈云荣%朱黎明
符代炎%戴愛國%鬍瑞成%田華%陳雲榮%硃黎明
부대염%대애국%호서성%전화%진운영%주려명
高血压,肺性%缺氧%Siah1
高血壓,肺性%缺氧%Siah1
고혈압,폐성%결양%Siah1
Hypertension,pulmonary%Anoxia%Siah1
目的：探讨低氧性肺动脉高压（ HPH）患者肺小动脉壁Siah1表达情况及其临床意义。方法收集湖南省肿瘤医院胸外科2008-04-01至2009-03-31行肺叶切除术的肺鳞癌患者36例，将除肺鳞癌外无其他肺部疾病患者12例纳入对照组，COPD非肺动脉高压患者12例纳入COPD组，COPD并HPH患者12例纳入PH组。收集患者肺组织，观察其肺血管重塑指标〔肺小动脉管壁厚度/血管外径（ WT%）、肺小动脉管壁面积/管总面积（ WA%）、肺小动脉管腔面积/管总面积（ LA%）〕，采用原位杂交法与免疫组织化学法检测肺小动脉壁Siah1 mRNA及其蛋白表达情况。结果光镜下可见对照组患者肺小动脉管壁薄，管腔大；COPD组患者肺小动脉管壁出现增厚，管腔狭窄；PH组患者肺小动脉管壁厚度进一步增加，管腔明显变窄。COPD组患者WA%、WT%高于对照组，LA%低于对照组（ P<0.05）；PH组患者WA%、WT%高于COPD组，LA%低于COPD组（P<0.05）。原位杂交结果显示，对照组患者肺小动脉壁Siah1 mRNA呈低表达，COPD组、PH组患者肺小动脉壁Siah1 mRNA表达逐渐增高。免疫组织化学结果显示，对照组患者肺小动脉壁Siah1蛋白呈低表达，COPD组、PH组患者肺小动脉壁Siah1蛋白表达逐渐增高。PH组患者肺小动脉壁Siah1 mRNA相对表达量及其蛋白水平高于COPD组，COPD组高于对照组（ P<0.05）。直线相关分析结果显示，Siah1蛋白水平与Siah1 mRNA相对表达量呈正相关（ r =0.873，P <0.01）；Siah1 mRNA 相对表达量与 WA%、WT%、肺动脉收缩压（PASP）均呈正相关（r 值分别为0.797、0.845、0.728，P <0.01），Siah1蛋白水平亦与WA%、WT%、PASP呈正相关（r值分别为0.791、0.791、0.704，P<0.01）。结论 HPH患者肺小动脉壁Siah1呈高表达，且肺小动脉壁Siah1表达与低氧性肺血管重塑及PASP有关，可能机制为慢性肺泡性低氧上调患者肺小动脉壁Siah1表达，进而参与HPH的发病。
目的：探討低氧性肺動脈高壓（ HPH）患者肺小動脈壁Siah1錶達情況及其臨床意義。方法收集湖南省腫瘤醫院胸外科2008-04-01至2009-03-31行肺葉切除術的肺鱗癌患者36例，將除肺鱗癌外無其他肺部疾病患者12例納入對照組，COPD非肺動脈高壓患者12例納入COPD組，COPD併HPH患者12例納入PH組。收集患者肺組織，觀察其肺血管重塑指標〔肺小動脈管壁厚度/血管外徑（ WT%）、肺小動脈管壁麵積/管總麵積（ WA%）、肺小動脈管腔麵積/管總麵積（ LA%）〕，採用原位雜交法與免疫組織化學法檢測肺小動脈壁Siah1 mRNA及其蛋白錶達情況。結果光鏡下可見對照組患者肺小動脈管壁薄，管腔大；COPD組患者肺小動脈管壁齣現增厚，管腔狹窄；PH組患者肺小動脈管壁厚度進一步增加，管腔明顯變窄。COPD組患者WA%、WT%高于對照組，LA%低于對照組（ P<0.05）；PH組患者WA%、WT%高于COPD組，LA%低于COPD組（P<0.05）。原位雜交結果顯示，對照組患者肺小動脈壁Siah1 mRNA呈低錶達，COPD組、PH組患者肺小動脈壁Siah1 mRNA錶達逐漸增高。免疫組織化學結果顯示，對照組患者肺小動脈壁Siah1蛋白呈低錶達，COPD組、PH組患者肺小動脈壁Siah1蛋白錶達逐漸增高。PH組患者肺小動脈壁Siah1 mRNA相對錶達量及其蛋白水平高于COPD組，COPD組高于對照組（ P<0.05）。直線相關分析結果顯示，Siah1蛋白水平與Siah1 mRNA相對錶達量呈正相關（ r =0.873，P <0.01）；Siah1 mRNA 相對錶達量與 WA%、WT%、肺動脈收縮壓（PASP）均呈正相關（r 值分彆為0.797、0.845、0.728，P <0.01），Siah1蛋白水平亦與WA%、WT%、PASP呈正相關（r值分彆為0.791、0.791、0.704，P<0.01）。結論 HPH患者肺小動脈壁Siah1呈高錶達，且肺小動脈壁Siah1錶達與低氧性肺血管重塑及PASP有關，可能機製為慢性肺泡性低氧上調患者肺小動脈壁Siah1錶達，進而參與HPH的髮病。
목적：탐토저양성폐동맥고압（ HPH）환자폐소동맥벽Siah1표체정황급기림상의의。방법수집호남성종류의원흉외과2008-04-01지2009-03-31행폐협절제술적폐린암환자36례，장제폐린암외무기타폐부질병환자12례납입대조조，COPD비폐동맥고압환자12례납입COPD조，COPD병HPH환자12례납입PH조。수집환자폐조직，관찰기폐혈관중소지표〔폐소동맥관벽후도/혈관외경（ WT%）、폐소동맥관벽면적/관총면적（ WA%）、폐소동맥관강면적/관총면적（ LA%）〕，채용원위잡교법여면역조직화학법검측폐소동맥벽Siah1 mRNA급기단백표체정황。결과광경하가견대조조환자폐소동맥관벽박，관강대；COPD조환자폐소동맥관벽출현증후，관강협착；PH조환자폐소동맥관벽후도진일보증가，관강명현변착。COPD조환자WA%、WT%고우대조조，LA%저우대조조（ P<0.05）；PH조환자WA%、WT%고우COPD조，LA%저우COPD조（P<0.05）。원위잡교결과현시，대조조환자폐소동맥벽Siah1 mRNA정저표체，COPD조、PH조환자폐소동맥벽Siah1 mRNA표체축점증고。면역조직화학결과현시，대조조환자폐소동맥벽Siah1단백정저표체，COPD조、PH조환자폐소동맥벽Siah1단백표체축점증고。PH조환자폐소동맥벽Siah1 mRNA상대표체량급기단백수평고우COPD조，COPD조고우대조조（ P<0.05）。직선상관분석결과현시，Siah1단백수평여Siah1 mRNA상대표체량정정상관（ r =0.873，P <0.01）；Siah1 mRNA 상대표체량여 WA%、WT%、폐동맥수축압（PASP）균정정상관（r 치분별위0.797、0.845、0.728，P <0.01），Siah1단백수평역여WA%、WT%、PASP정정상관（r치분별위0.791、0.791、0.704，P<0.01）。결론 HPH환자폐소동맥벽Siah1정고표체，차폐소동맥벽Siah1표체여저양성폐혈관중소급PASP유관，가능궤제위만성폐포성저양상조환자폐소동맥벽Siah1표체，진이삼여HPH적발병。
Objective To investigate the expression of Siah1 of pulmonary arteriole walls and its significance in patients with hypoxic pulmonary hypertension. Methods From 2008-04-01 to 2009-03-31,a total of 36 lung squamous carcinoma patients undergoing pulmonary lobectomy were selected in the Department of Thoracic Surgery,Hu'nan Provincial Tumor Hospital,thereinto 12 patients without other pulmonary disease were served as A group,12 patients with COPD were served as B group,12 patients with COPD and hypoxic pulmonary hypertension were served as C group. Pulmonary vascular remodeling index including WT%,WA%,LA% was compared among the three groups;insitu hybridization method was used to detect the Siah1 mRNA expression,and immunohistochemical method was used to detect the Siah1 protein expression. Results Light microscope found that,the pulmonary arteriole walls of A group was thin,and the lumen was big;the pulmonary arteriole walls of B group was thickening,and the lumen was narrow;the pulmonary arteriole walls of C group was more thickening than that of B group,and the lumen was obviously narrow. WA% and WT% of B group were statistically significantly higher than those of A group,while LA% of B group was statistically significantly lower than that of A group(P<0. 05);WA% and WT% of C group were statistically significantly higher than those of B group,while LA% of C group was statistically significantly lower than that of B group(P <0. 05). By insitu hybridization method and immunohistochemical method,Siah1 mRNA expression and protein expression of A group were low,and those of groups B and C were increasingly higher. mRNA relative expression and protein level of Siah1 of C group were statistically significantly higher than those of B group, and those of B group were statistically significantly higher than those of A group(P <0. 05). Linear correlation analysis showed that,mRNA relative expression of Siah1 was positively correlated with protein level of Siah1(r=0. 873,P<0. 01);mRNA relative expression of Siah1 was positively correlated with WA%,WT%,PASP,respectively(r =0. 797,0. 845,0. 728,P <0. 01);protein level of Siah1 was positively correlated with WA%,WT%,PASP,respectively(r =0. 791,0. 791,0. 704,P <0. 01). Conclusion The expression of Siah1 of pulmonary arteriole walls is high in patients with hypoxic pulmonary hypertension,and it is correlated with hypoxic pulmonary vascular remodeling and PASP;chronic alveolar hypoxia may induce the expression of Siah1 of pulmonary arteriole walls,and lead to hypoxic pulmonary hypertension at last.
