广州化工
廣州化工
엄주화공
GUANGZHOU CHEMICAL INDUSTRY AND TECHNOLOGY
2015年
12期
106-108
,共3页
冯涛%刘鹏%刘海燕%锁然
馮濤%劉鵬%劉海燕%鎖然
풍도%류붕%류해연%쇄연
虎杖%白藜芦醇%大黄素%高效液相色谱%双波长
虎杖%白藜蘆醇%大黃素%高效液相色譜%雙波長
호장%백려호순%대황소%고효액상색보%쌍파장
polygonum cuspidatum%resveratrol%emodin%HPLC%double wavelength
以乙腈和水为流动相，流速1 mL／min，检测波长为306 nm和440 nm，建立了同时测定虎杖提取液中的反式白藜芦醇、反式白藜芦醇苷、大黄素含量的高效液相色谱法。结果表明：在建立的色谱条件下，反式白藜芦醇及苷与大黄素的线性范围为0.12～20 mg／L，0.15～20 mg／L，0.10～20 mg／L。在此范围内各物质的线性关系良好。最低检出限分别为0.8 mg／L、0.7 mg／L、0.75 mg／L，平均回收率分别为100.2％、100.8％、103.3％， RSD分别为1.197％、1.466％、2.469％。
以乙腈和水為流動相，流速1 mL／min，檢測波長為306 nm和440 nm，建立瞭同時測定虎杖提取液中的反式白藜蘆醇、反式白藜蘆醇苷、大黃素含量的高效液相色譜法。結果錶明：在建立的色譜條件下，反式白藜蘆醇及苷與大黃素的線性範圍為0.12～20 mg／L，0.15～20 mg／L，0.10～20 mg／L。在此範圍內各物質的線性關繫良好。最低檢齣限分彆為0.8 mg／L、0.7 mg／L、0.75 mg／L，平均迴收率分彆為100.2％、100.8％、103.3％， RSD分彆為1.197％、1.466％、2.469％。
이을정화수위류동상，류속1 mL／min，검측파장위306 nm화440 nm，건립료동시측정호장제취액중적반식백려호순、반식백려호순감、대황소함량적고효액상색보법。결과표명：재건립적색보조건하，반식백려호순급감여대황소적선성범위위0.12～20 mg／L，0.15～20 mg／L，0.10～20 mg／L。재차범위내각물질적선성관계량호。최저검출한분별위0.8 mg／L、0.7 mg／L、0.75 mg／L，평균회수솔분별위100.2％、100.8％、103.3％， RSD분별위1.197％、1.466％、2.469％。
The determination of the trans knotweed extract resveratrol was established , trans resveratrol glucoside , emodin content in high performance liquid chromatography ( HPLC) at the same time with acetonitrile and water as mobile phase, flow rate of 1 mL/min, detection wavelength of 306 nm and 440 nm.The research showed under the established conditions, linear range of trans-resveratrol and glucoside and emodin were 0.12 ~20 mg/L, 0.15 ~20 mg/L and 0.10~20 mg/L.Linear relationship of the matter were good in the rage.The limit of detection were 0.8 μg/mL, 0.7 μg/mL and 0.75 μg/mL, the average recovery rate were 100.2%, 100.8% and 103.3%, RSD were 1.197%, 1.466%and 2.469%.
