CAJ | 학술논문

目的：探讨唐山地区乙型肝炎病毒(HBV)基因型与慢性乙型肝炎患者临床表现类型、HBV 感染者的肝功能,乙型肝炎标志物及 HBV DNA载量之间的相互关系。方法使用毛细管电泳仪应用双脱氧终止法测序技术对239例乙型肝炎患者进行 HBV基因分型的检测,并对于乙型肝炎病毒不同基因型进行肝功能、乙型肝炎病毒标志物和乙型肝炎病毒DNA载量进行相关性分析。结果唐山地区乙型肝炎患者的239例样本中未检出基因型5例(2.1%)。已检测出乙型肝炎病毒基因型的234例样本中共检出B、C、D 3种基因型,其中B基因型30例12.8%,C基因型203例86.8%,D基因型1例0.4%,C基因型为主要基因型。C基因型感染者与 B基因型血清中的乙型肝炎病毒 e抗原(HBeAg)阳性率相比差异无统计学意义；而C基因型感染者血清中抗-HBe阳性率显著低于B基因型(χ2=13.093,P<0.01)。C基因型在肝硬化和乙型肝炎病毒相关性肝细胞癌中所占的比例均大于B基因型,但差异无统计学意义(P均>0.05)。结论乙型肝炎患者肝脏损伤严重程度与 C基因型相关度较高。
목적：탐토당산지구을형간염병독(HBV)기인형여만성을형간염환자림상표현류형、HBV 감염자적간공능,을형간염표지물급 HBV DNA재량지간적상호관계。방법사용모세관전영의응용쌍탈양종지법측서기술대239례을형간염환자진행 HBV기인분형적검측,병대우을형간염병독불동기인형진행간공능、을형간염병독표지물화을형간염병독DNA재량진행상관성분석。결과당산지구을형간염환자적239례양본중미검출기인형5례(2.1%)。이검측출을형간염병독기인형적234례양본중공검출B、C、D 3충기인형,기중B기인형30례12.8%,C기인형203례86.8%,D기인형1례0.4%,C기인형위주요기인형。C기인형감염자여 B기인형혈청중적을형간염병독 e항원(HBeAg)양성솔상비차이무통계학의의；이C기인형감염자혈청중항-HBe양성솔현저저우B기인형(χ2=13.093,P<0.01)。C기인형재간경화화을형간염병독상관성간세포암중소점적비례균대우B기인형,단차이무통계학의의(P균>0.05)。결론을형간염환자간장손상엄중정도여 C기인형상관도교고。
Objective To investigate the relationship of the HBV genotype with clinical manifestations,liv-er function of HBV infection,HBV markers and HBV DNA load of chronic patients with hepatitis B in Tangshan region.Methods The sera from 239 cases of patients with hepatitis B were genotyped by a capillary electrophore-sis instrument and the dideoxy sequencing technology,and we analyzed the correlation between liver function, HBV markers and HBV DNA load of different genotypes of hepatitis B virus.Results Genotype of 5 cases of pa-tients (2.1%)was not determined in 239 patients with hepatitis B.234 cases of patients detected with hepatitis B genotype were determined three genotypes B,C and D,including 30 cases of genotype B(12.82%),203 cases (86.75%)for C genotype, 1 case (0.43%)for D genotype. C genotype was dominant in Tangshan region. There was no significant difference for HBeAg positive rate between C genotype and B genotype;The positive rate of anti-HBe in C genotype was significantly lower than that in B genotype,the difference was statistically signifi-cant(χ2 =13.093,P<0.01).C genotypes of hepatitis B virus was related to cirrhosis and hepatocellular carcino-ma,the proportion of which was greater than that of genotype B,but the difference was not statistically signifi-cant (P>0.05).Conclusion The severity of liver damage in patients with hepatitis C genotype is higher.