中国烟草学报
中國煙草學報
중국연초학보
ACTA TABACARIA SINICA
2015年
3期
82-87
,共6页
杨志晓%薛刚%丁燕芳%张小全%刘红峰%张杰%王轶%任学良%杨铁钊
楊誌曉%薛剛%丁燕芳%張小全%劉紅峰%張傑%王軼%任學良%楊鐵釗
양지효%설강%정연방%장소전%류홍봉%장걸%왕질%임학량%양철쇠
烟草%烟草赤星病%光合色素%光合作用%渗透调节
煙草%煙草赤星病%光閤色素%光閤作用%滲透調節
연초%연초적성병%광합색소%광합작용%삼투조절
tobacco%tobacco brown spot%photosynthetic pigment%photosynthesis%osmotic regulation
以抗烟草赤星病品种净叶黄(JYH)和感病品种长脖黄(CBH)为材料,在盆栽试验条件下,研究不同烟草赤星病胁迫程度(轻度胁迫、中度胁迫和重度胁迫)对光合特性和渗透调节的影响。结果显示:⑴烟草赤星病胁迫导致2个品种的叶绿素a、叶绿素b、总叶绿素和类胡萝卜素含量均呈下降趋势,且JYH 的降幅小于CBH。⑵ JYH、CBH 的净光合速率、气孔导度因烟草赤星病胁迫而降低。2个品种的胞间CO2浓度、气孔限制值变化具有明显差异,烟草赤星病胁迫导致CBH 的胞间CO2浓度上升,气孔限制值则明显下降,这与JYH 在重度胁迫下的变化趋势一致。而JYH 的胞间CO2浓度在轻度、中度胁迫降低,气孔限制值则呈上升趋势。⑶与CBH 相比,JYH 在烟草赤星病胁迫下具有较高的脯氨酸和可溶性总糖含量,较低的MDA 含量。研究结果表明,JYH 具有较强的光合作用和渗透物质调节能力,是对烟草赤星病胁迫具有较强抗性的生理基础。
以抗煙草赤星病品種淨葉黃(JYH)和感病品種長脖黃(CBH)為材料,在盆栽試驗條件下,研究不同煙草赤星病脅迫程度(輕度脅迫、中度脅迫和重度脅迫)對光閤特性和滲透調節的影響。結果顯示:⑴煙草赤星病脅迫導緻2箇品種的葉綠素a、葉綠素b、總葉綠素和類鬍蘿蔔素含量均呈下降趨勢,且JYH 的降幅小于CBH。⑵ JYH、CBH 的淨光閤速率、氣孔導度因煙草赤星病脅迫而降低。2箇品種的胞間CO2濃度、氣孔限製值變化具有明顯差異,煙草赤星病脅迫導緻CBH 的胞間CO2濃度上升,氣孔限製值則明顯下降,這與JYH 在重度脅迫下的變化趨勢一緻。而JYH 的胞間CO2濃度在輕度、中度脅迫降低,氣孔限製值則呈上升趨勢。⑶與CBH 相比,JYH 在煙草赤星病脅迫下具有較高的脯氨痠和可溶性總糖含量,較低的MDA 含量。研究結果錶明,JYH 具有較彊的光閤作用和滲透物質調節能力,是對煙草赤星病脅迫具有較彊抗性的生理基礎。
이항연초적성병품충정협황(JYH)화감병품충장발황(CBH)위재료,재분재시험조건하,연구불동연초적성병협박정도(경도협박、중도협박화중도협박)대광합특성화삼투조절적영향。결과현시:⑴연초적성병협박도치2개품충적협록소a、협록소b、총협록소화류호라복소함량균정하강추세,차JYH 적강폭소우CBH。⑵ JYH、CBH 적정광합속솔、기공도도인연초적성병협박이강저。2개품충적포간CO2농도、기공한제치변화구유명현차이,연초적성병협박도치CBH 적포간CO2농도상승,기공한제치칙명현하강,저여JYH 재중도협박하적변화추세일치。이JYH 적포간CO2농도재경도、중도협박강저,기공한제치칙정상승추세。⑶여CBH 상비,JYH 재연초적성병협박하구유교고적포안산화가용성총당함량,교저적MDA 함량。연구결과표명,JYH 구유교강적광합작용화삼투물질조절능력,시대연초적성병협박구유교강항성적생리기출。
Responses of photosynthetic characteristics and osmotic regulation were studied under three different stress levels of tobacco brown spot at pot experiment condition, and two tobacco cultivars with different resistance to tobacco brown spot including a resistant cultivar Jingyehuang (JYH) and sensitive one Changbohuang (CBH) were selected as experimental material. Results showed that tobacco brown spot stress decreased chlorophyll a, chlorophyll b, total chlorophyll and carotenoids in the two cultivars. In addition, the decreased range of photosynthetic pigments was less in JYH than that in CBH. Both the net photosynthetic rate and the stomatal conductance were inhibited under tobacco brown spot stress. However, the net photosynthetic rate of JYH increased under mild stress whereas there was a decrease under moderate and severe stress. In the two cultivars, changes in intercellular CO2 concentration and stomatal conductance were divergent. The intercellular CO2 concentration in CBH tended to rise under the three stress levels, whereas the stomatal limitation value showed a significant decrease. In JYH under severe stress, the photosynthetic parameters consisting of both intercellular CO2 concentration and stomatal conductance showed the same trend as in CBH. However, in contrast with the rising trend of intercellular CO2 concentration under severe stress, the stomatal limitation value of JYH decreased under both mild and moderate stress. Compared with CBH, JYH showed higher content of total soluble sugar and proline and lower content of MDA under three stress levels. These results indicate that, JYH featured higher photosynthetic performance and betterosmotic adjustment, which formed the physiological basis for strong resistance to tobacco brown spot stress.