中国实验方剂学杂志
中國實驗方劑學雜誌
중국실험방제학잡지
CHINESE JOURNAL OF EXPERIMENTAL TRADITIONAL MEDICAL FORMULAE
2015年
12期
36-39
,共4页
卫阳飞%戚欢阳%宋海%岳国仁
衛暘飛%慼歡暘%宋海%嶽國仁
위양비%척환양%송해%악국인
金刚藤软胶囊%绿原酸%白藜芦醇%芦丁%槲皮素%山柰酚%高效液相色谱法%双波长检测
金剛籐軟膠囊%綠原痠%白藜蘆醇%蘆丁%槲皮素%山柰酚%高效液相色譜法%雙波長檢測
금강등연효낭%록원산%백려호순%호정%곡피소%산내분%고효액상색보법%쌍파장검측
Jingangteng soft capsule%chlorogenic acid%resveratrol%rutin%quercetin%kaempferol%HPLC%dual-wavelength
目的:建立双波长HPLC同时测定金刚藤软胶囊中绿原酸、白藜芦醇、芦丁、槲皮素和山柰酚含量的方法.方法:采用Agilent ZORBAX SB-C18色谱柱(4.6 mm×150 mm,5μm),流动相乙腈-0.1％磷酸水溶液梯度洗脱,流速1.0 mL·min-,柱温25℃,检测波长306 nm(绿原酸、白藜芦醇)和365 nm(芦丁、槲皮素、山柰酚).结果:绿原酸、白藜芦醇、芦丁、槲皮素、山柰酚分别在0.058 5～2.34 μg(r =0.999 8),0.025 3～1.01 μg(r =0.999 9),0.042 2～ 0.844μg(r=0.999 6),0.018 1 ～0.722 μg(r=0.999 9),0.0165 ～0.660μg(r =0.999 9)呈现良好的线性关系,平均回收率分别为98.0％,100.6％,96.0％,102.7％,98.5％,RSD分别为1.2％,2.2％,1.8％,2.9％,1.3％.结论:该方法操作简便、重复性好,可作为金刚藤软胶囊中5个活性成分的含量测定方法.
目的:建立雙波長HPLC同時測定金剛籐軟膠囊中綠原痠、白藜蘆醇、蘆丁、槲皮素和山柰酚含量的方法.方法:採用Agilent ZORBAX SB-C18色譜柱(4.6 mm×150 mm,5μm),流動相乙腈-0.1％燐痠水溶液梯度洗脫,流速1.0 mL·min-,柱溫25℃,檢測波長306 nm(綠原痠、白藜蘆醇)和365 nm(蘆丁、槲皮素、山柰酚).結果:綠原痠、白藜蘆醇、蘆丁、槲皮素、山柰酚分彆在0.058 5～2.34 μg(r =0.999 8),0.025 3～1.01 μg(r =0.999 9),0.042 2～ 0.844μg(r=0.999 6),0.018 1 ～0.722 μg(r=0.999 9),0.0165 ～0.660μg(r =0.999 9)呈現良好的線性關繫,平均迴收率分彆為98.0％,100.6％,96.0％,102.7％,98.5％,RSD分彆為1.2％,2.2％,1.8％,2.9％,1.3％.結論:該方法操作簡便、重複性好,可作為金剛籐軟膠囊中5箇活性成分的含量測定方法.
목적:건립쌍파장HPLC동시측정금강등연효낭중록원산、백려호순、호정、곡피소화산내분함량적방법.방법:채용Agilent ZORBAX SB-C18색보주(4.6 mm×150 mm,5μm),류동상을정-0.1％린산수용액제도세탈,류속1.0 mL·min-,주온25℃,검측파장306 nm(록원산、백려호순)화365 nm(호정、곡피소、산내분).결과:록원산、백려호순、호정、곡피소、산내분분별재0.058 5～2.34 μg(r =0.999 8),0.025 3～1.01 μg(r =0.999 9),0.042 2～ 0.844μg(r=0.999 6),0.018 1 ～0.722 μg(r=0.999 9),0.0165 ～0.660μg(r =0.999 9)정현량호적선성관계,평균회수솔분별위98.0％,100.6％,96.0％,102.7％,98.5％,RSD분별위1.2％,2.2％,1.8％,2.9％,1.3％.결론:해방법조작간편、중복성호,가작위금강등연효낭중5개활성성분적함량측정방법.
