CAJ | 학술논문

以植物源抗病毒剂VFB 为基础，经配方改造和工艺改进，优化出了植物源抗病毒剂VFB-1。为探究VFB-1对烟草花叶病的防治作用，本研究采用半叶枯斑法测定了VFB-1对TMV 的体外钝化、初侵染和复制增殖抑制活性；采用盆栽法和田间小区试验评价了VFB-1对烟草花叶病毒病的预防及治疗效果。结果表明，VFB-1对烟草花叶病毒(TMV)具有明显的体外钝化作用，钝化30min 后，抑制率达82.77%；对TMV 的初侵染和复制增殖具有较好的抑制作用，24 h 的抑制率分别达到69.80%和54.07%?盆栽药效试验中，VFB-1100倍稀释液的预防效果为70.23%，治疗效果为35.70%；田间药效试验表明，VFB-1对烟草花叶病毒病有较好的预防和治疗作用，其200倍稀释液药后21d 的预防效果仍为40%以上，400倍液的预防效果明显高于VFB，且与病毒A（20%吗啉胍?乙铜WP）相当；其200倍液14d 的治疗效果显著高于VFB（200倍稀释液）和病毒A（400倍稀释液）处理。可见，植物源抗病毒剂VFB-1对TMV 具有良好的预防和治疗效果，开发前景良好?
이식물원항병독제VFB 위기출，경배방개조화공예개진，우화출료식물원항병독제VFB-1。위탐구VFB-1대연초화협병적방치작용，본연구채용반협고반법측정료VFB-1대TMV 적체외둔화、초침염화복제증식억제활성；채용분재법화전간소구시험평개료VFB-1대연초화협병독병적예방급치료효과。결과표명，VFB-1대연초화협병독(TMV)구유명현적체외둔화작용，둔화30min 후，억제솔체82.77%；대TMV 적초침염화복제증식구유교호적억제작용，24 h 적억제솔분별체도69.80%화54.07%?분재약효시험중，VFB-1100배희석액적예방효과위70.23%，치료효과위35.70%；전간약효시험표명，VFB-1대연초화협병독병유교호적예방화치료작용，기200배희석액약후21d 적예방효과잉위40%이상，400배액적예방효과명현고우VFB，차여병독A（20%마람고?을동WP）상당；기200배액14d 적치료효과현저고우VFB（200배희석액）화병독A（400배희석액）처리。가견，식물원항병독제VFB-1대TMV 구유량호적예방화치료효과，개발전경량호?
Effect of VFB-I on tobacco mosaic virus (TMV) was evaluated with anti-TMV activities tested in vitro by local lesion. Protection and treatment efficiencies of VFB-I on TMV were evaluated by pot experiment and field plot trials. Results showed that VFB displayed significant inactivation on TMV with inhibition rate of 82.75% after mixing VFB with TMV for 0.5 h. Infection and replication of TMV to Nicotiana glutinosa were also inhibited by VFB-I, with inhibition rate of 69.80% and 54.06%, respectively. Pot experiment showed that protection efficiency of VFB-Ⅰagainst TMV disease was 70.24% and remedial efficiency was 35.71% by spraying with VFB-Ⅰ diluent of 1:100. In field trials, protection efficiency of VFB-Ⅰdiluent of 1:400 on TMV disease was higher than that of VFB, but equivalent to that of 20% moroxydine+cupric acetate WP at the same concentration. The remedial effect of VFB-Ⅰ(diluent of 1:200) was much stronger than that of 20% moroxydine+cupric acetate WP (diluents of 1:400) and VFB (diluents of 1:200). VFB-Ⅰ showed higher anti-TMV activity indicating that it is effective in controlling TMV disease.