西藏大学学报(自然科学版)
西藏大學學報(自然科學版)
서장대학학보(자연과학판)
JOURNAL OF TIBET UNIVERSITY
2015年
1期
35-38
,共4页
冰川棘豆%半知菌%曲霉属
冰川棘豆%半知菌%麯黴屬
빙천극두%반지균%곡매속
Oxyropis glacialis%Deuteromycotina%Aspergillus
为探讨西藏植物内生菌曲霉属真菌的生物学特性,本研究利用PDA培养基和KB培养基鉴定了菌落的特征,利用载片培养法鉴定了孢子的特征。结果表明:①菌株在PDA培养基上菌落散生,边缘不规则缺裂,稀薄而透明,基部菌丝体薄且平坦、具沟纹、丝绒状质地;在KB培养基上菌落为黑色,边缘不规则缺裂,且形成有规则的褶皱,稀薄而透明,基部菌丝体薄、平坦、具沟纹、丝绒状质地。②所分离出的菌株不具有有性型。③分离得到的菌株属于半知菌亚门丝孢纲丝孢目淡色孢科曲霉属环绕亚属黑色组真菌的一种。
為探討西藏植物內生菌麯黴屬真菌的生物學特性,本研究利用PDA培養基和KB培養基鑒定瞭菌落的特徵,利用載片培養法鑒定瞭孢子的特徵。結果錶明:①菌株在PDA培養基上菌落散生,邊緣不規則缺裂,稀薄而透明,基部菌絲體薄且平坦、具溝紋、絲絨狀質地;在KB培養基上菌落為黑色,邊緣不規則缺裂,且形成有規則的褶皺,稀薄而透明,基部菌絲體薄、平坦、具溝紋、絲絨狀質地。②所分離齣的菌株不具有有性型。③分離得到的菌株屬于半知菌亞門絲孢綱絲孢目淡色孢科麯黴屬環繞亞屬黑色組真菌的一種。
위탐토서장식물내생균곡매속진균적생물학특성,본연구이용PDA배양기화KB배양기감정료균락적특정,이용재편배양법감정료포자적특정。결과표명:①균주재PDA배양기상균락산생,변연불규칙결렬,희박이투명,기부균사체박차평탄、구구문、사융상질지;재KB배양기상균락위흑색,변연불규칙결렬,차형성유규칙적습추,희박이투명,기부균사체박、평탄、구구문、사융상질지。②소분리출적균주불구유유성형。③분리득도적균주속우반지균아문사포강사포목담색포과곡매속배요아속흑색조진균적일충。
The biological characteristics of endophytic fungus belonging to Aspergillus separated from fresh roots of Oxyropi glacialis was studied and the characteristics of colonies and spores were identified by using photo cul?ture method with PDA medium and KB. The results showed that:①colonies of the strain were scattered, thin and transparent with irregular edges, thin and flat base mycelium with grooves and velvet-like texture on both PDA and KB medium, and colonies were black with regular folds on KB medium;②the isolated strain had no te?leomorph;③the isolated strain was one of the species of black fungus group belonging to Aspergillus, Monilia?ceous, Hyphomycetales, Hyphomycetes, Deuteromycotina.