CAJ | 학술논문

[目的]研究黄芪注射液对脓毒症大鼠肠道黏膜上皮细胞的保护作用及其机制。[方法] Sprague-Dawley大鼠30只随机分为假手术组、脓毒症模型组、黄芪注射液干预组，每组10只。脓毒症模型采用盲肠结扎穿孔法构建，黄芪注射液干预组大鼠在术前15 min和术后6 h接受800 mg·kg-1黄芪注射液的干预。术后24 h取大鼠肠道黏膜组织-80℃冰冻保存。采用TUNEL法检测各组大鼠肠道黏膜上皮细胞的凋亡情况，实时荧光定量PCR（qPCR）和Western blot技术检测各组大鼠肠道黏膜组织的Bcl-2和Bax表达水平。[结果]假手术组、脓毒症模型组和黄芪注射液干预组的大鼠肠道黏膜上皮细胞凋亡指数分别为(5.2±0.3)，(22.3±1.2)和(11.2±0.9)。脓毒症模型组的凋亡指数明显高于假手术组（P<0.05），黄芪注射液干预组的凋亡指数明显低于脓毒症模型组（P<0.05）。脓毒症模型组Bcl-2表达水平低于假手术组，Bax表达水平高于假手术组（P<0.05）。黄芪注射液干预组Bcl-2表达水平高于脓毒症模型组，Bax表达水平低于脓毒症模型组（P<0.05）。[结论]黄芪注射液可以通过维持Bcl-2/Bax平衡来抑制脓毒症引起的肠道黏膜上皮细胞凋亡。
[목적]연구황기주사액대농독증대서장도점막상피세포적보호작용급기궤제。[방법] Sprague-Dawley대서30지수궤분위가수술조、농독증모형조、황기주사액간예조，매조10지。농독증모형채용맹장결찰천공법구건，황기주사액간예조대서재술전15 min화술후6 h접수800 mg·kg-1황기주사액적간예。술후24 h취대서장도점막조직-80℃빙동보존。채용TUNEL법검측각조대서장도점막상피세포적조망정황，실시형광정량PCR（qPCR）화Western blot기술검측각조대서장도점막조직적Bcl-2화Bax표체수평。[결과]가수술조、농독증모형조화황기주사액간예조적대서장도점막상피세포조망지수분별위(5.2±0.3)，(22.3±1.2)화(11.2±0.9)。농독증모형조적조망지수명현고우가수술조（P<0.05），황기주사액간예조적조망지수명현저우농독증모형조（P<0.05）。농독증모형조Bcl-2표체수평저우가수술조，Bax표체수평고우가수술조（P<0.05）。황기주사액간예조Bcl-2표체수평고우농독증모형조，Bax표체수평저우농독증모형조（P<0.05）。[결론]황기주사액가이통과유지Bcl-2/Bax평형래억제농독증인기적장도점막상피세포조망。
Objective] To investigate the protective effect and potential mechanism of Astragalus injection on mucosa epithelial cells in rats with sepsis . [Methods] Thirty Sprague-Dawley rats were randomly divided into sham,model and Astragalus injection treatment groups with 10 rats in each group.Rat sepsis model was constructed by cecal ligation and puncture.Rats in the Astragalus injection treatment group received 800 mg·kg-1 of Astragalus injection at 15 minutes before and 6 hours after operation. Intestinal mucosa were harvested and frozen at -80℃ on post-operation 24 hours. Intestinal mucosa epithelial cell apoptosis was detected by TUNEL,while Bcl-2 and Bax levels were determined by real-time quantitative PCR(qPCR) and Western blot technique.[Results] The apoptosis indexes were 5.2±0.3,22.3±1.2 and 11.2±0.9 of sham,model and Astragalus injection treatment group,respectively. Apoptosis index in model group was significantly higher than that in sham group( P<0.05),while the apoptosis of mucosa epithelial cells was significantly attenuated after Astragalus injection treatment(P<0.05).In model group,the expression of Bcl-2 was lower than that of sham group,whereas the expression of Bax was higher than that in sham group(P<0.05).Astragalus injection treatment significantly lowered Bax level while elevated Bcl-2 expression, compared with model group(P<0.05). [Conclusion] Astragalus injection could suppress sepsis-induced apoptosis of mucosa epithelial cells via up-regulating Bcl-2 and down-regulating Bax.