CAJ | 학술논문

目的：探讨艾灸对大鼠脑缺血再灌注后内源性神经干细胞分化的影响，明确艾灸治疗脑缺血性卒中的作用机制，为艾灸治疗缺血性卒中提供新的理论依据。方法：选用Wistar大鼠72只，通过改良线栓法制备MCAO模型，将大鼠随机分为假手术组、模型组、艾灸组。每组分3天、7天、14天、21天4个时间点，每个时间点6只。通过腹腔注射Brdu来标记增殖的细胞。取大鼠“百会、太阳（双）”穴进行实验研究。通过TTC染色和HE染色来观察大鼠脑组织的病理学变化。应用免疫荧光技术，进行BrdU／GFAP、BrdU／NSE双标染色。通过显微图像分析系统对阳性结果进行分析。结果：艾灸组各时间点神经功能缺损评分与模型组比较，评分降低，具有显著性差异（ P＜0．05）。同一时间点艾灸组神经功能缺损评分与模型组比较有显著性差异（ P＜0．05）；艾灸可促使脑缺血损伤后BrdU／GFAP、BrdU／NSE双标阳性细胞增加，于缺血损伤后3天达高峰。与模型组及假手术组比较有显著性差异（ P＜0．05）。结论：艾灸能显著促进脑缺血再灌注后大鼠神经功能恢复；艾灸能促进脑缺血再灌注后脑组织BrdU／GFAP双标阳性细胞、BrdU／NSE双标阳性细胞的表达，表明了艾灸能够促进神经干细胞向神经元细胞和神经胶质细胞分化。
목적：탐토애구대대서뇌결혈재관주후내원성신경간세포분화적영향，명학애구치료뇌결혈성졸중적작용궤제，위애구치료결혈성졸중제공신적이론의거。방법：선용Wistar대서72지，통과개량선전법제비MCAO모형，장대서수궤분위가수술조、모형조、애구조。매조분3천、7천、14천、21천4개시간점，매개시간점6지。통과복강주사Brdu래표기증식적세포。취대서“백회、태양（쌍）”혈진행실험연구。통과TTC염색화HE염색래관찰대서뇌조직적병이학변화。응용면역형광기술，진행BrdU／GFAP、BrdU／NSE쌍표염색。통과현미도상분석계통대양성결과진행분석。결과：애구조각시간점신경공능결손평분여모형조비교，평분강저，구유현저성차이（ P＜0．05）。동일시간점애구조신경공능결손평분여모형조비교유현저성차이（ P＜0．05）；애구가촉사뇌결혈손상후BrdU／GFAP、BrdU／NSE쌍표양성세포증가，우결혈손상후3천체고봉。여모형조급가수술조비교유현저성차이（ P＜0．05）。결론：애구능현저촉진뇌결혈재관주후대서신경공능회복；애구능촉진뇌결혈재관주후뇌조직BrdU／GFAP쌍표양성세포、BrdU／NSE쌍표양성세포적표체，표명료애구능구촉진신경간세포향신경원세포화신경효질세포분화。
Objective:To investigate the effect of moxibustion on endogenous neural stem cell differentiation in rats after cerebral ischemia reperfusion,to clarify the mechanism of action of moxibustion in the treatment of cer-ebral ischemic stroke, and to provide theoretical basis for moxibustion therapy in the treatment of ischemic stroke.Methods:72 Wistar rats were prepared by modified thread embolism of middle cerebral artery focal cere-bral ischemia reperfusion model ( MCAO model) ,and they were randomly divided into a sham operation group, a model group and a moxibustion group.Every group was subdivided into 3d, 7d, 14d, 21d 4 time points and at each time point there were 6 rats.The intraperitoneal injection of Brdu was used to label proliferating cells. The ratsˊ“Baihui, Taiyang( double)” points were selected to conduct the experimental study.TTC staining and HE staining were used to understand brain ischemia and pathological brain tissue perfusion changes.Apply im-munofluorescence technique, BrdU/GFAP, BrdU/NSE double staining.Microscopic image analysis system was used to analyze the positive results.Results:In moxibustion group at each time point neural function defect score was compared with that of the model group and the score was decreased with a significant difference ( P <0.05 ) .At the same time point neural function defect score in the moxibustion group was compared with that in the model group,and there was a significant difference ( P<0.05);moxibustion could promote the increase of BrdU/GFAP, BrdU/NSE positive cells after cerebral ischemia injury, reaching the peak after 3 days.There was a significant difference compared with the model group and sham operation group (P<0.05).Conclusion:Moxibustion can significantly promote the recovery of neural function in rats with cerebral ischemia after reperfu-sion;moxibustion can promote the expression of brain tissue of cerebral ischemia and reperfusion BrdU/GFAP positive cells, BrdU/NSE positive cells, showing that moxibustion can promote neural stem cells into neurons and glial cell differentiation.