浙江医学
浙江醫學
절강의학
ZHEJIANG MEDICAL JOURNAL
2015年
12期
1051-1054
,共4页
子痫前期%调节性T细胞%ICOS%CCR6
子癇前期%調節性T細胞%ICOS%CCR6
자간전기%조절성T세포%ICOS%CCR6
Preeclampsia%Regulatory T cel%ICOS%CCR6
目的：探讨子痫前期患者CD4+CD25+调节性T细胞（Treg）亚群改变及在PE免疫耐受失衡中的作用。方法选取PE患者（观察组）30例，同期正常晚期妊娠（对照组）20例。分别采集PE患者和正常晚期妊娠妇女外周血，采用流式细胞术检测Treg以及活化记性相关（CD45RO、HLA- DR）、归巢相关（CCR4、CCR6、CD103）、功能相关（ICOS、CTLA-4、Galectin 1）和生存相关（PD1和CD95）标记分子的表达。采用免疫抑制实验检测外周血中 Treg 的免疫抑制功能。结果观察组患者外周血CD4+CD25+细胞的比例[(5.87±3.34)%]低于对照组[（6.65±1.18）%]，但差异无统计学意义。免疫抑制实验显示观察组患者外周血中Treg对CD4+CD25-T细胞的增值抑制作用与对照组无统计学差异。通过对Treg多个标记分子的检测进一步发现，ICOS和CCR6在观察组Treg上的表达水平显著低于对照组Treg（均P＜0.01）；CCR4和Galectin 1的表达水平较对照组低，而CD45RO、HLA- DR、CD103、CTLA、PD1和CD95的表达水平较对照组高，但两组间差异均无统计学意义（均P＞0.05）。结论 Treg亚群比例失调可能是导致子痫前期发生的重要因素。
目的：探討子癇前期患者CD4+CD25+調節性T細胞（Treg）亞群改變及在PE免疫耐受失衡中的作用。方法選取PE患者（觀察組）30例，同期正常晚期妊娠（對照組）20例。分彆採集PE患者和正常晚期妊娠婦女外週血，採用流式細胞術檢測Treg以及活化記性相關（CD45RO、HLA- DR）、歸巢相關（CCR4、CCR6、CD103）、功能相關（ICOS、CTLA-4、Galectin 1）和生存相關（PD1和CD95）標記分子的錶達。採用免疫抑製實驗檢測外週血中 Treg 的免疫抑製功能。結果觀察組患者外週血CD4+CD25+細胞的比例[(5.87±3.34)%]低于對照組[（6.65±1.18）%]，但差異無統計學意義。免疫抑製實驗顯示觀察組患者外週血中Treg對CD4+CD25-T細胞的增值抑製作用與對照組無統計學差異。通過對Treg多箇標記分子的檢測進一步髮現，ICOS和CCR6在觀察組Treg上的錶達水平顯著低于對照組Treg（均P＜0.01）；CCR4和Galectin 1的錶達水平較對照組低，而CD45RO、HLA- DR、CD103、CTLA、PD1和CD95的錶達水平較對照組高，但兩組間差異均無統計學意義（均P＞0.05）。結論 Treg亞群比例失調可能是導緻子癇前期髮生的重要因素。
목적：탐토자간전기환자CD4+CD25+조절성T세포（Treg）아군개변급재PE면역내수실형중적작용。방법선취PE환자（관찰조）30례，동기정상만기임신（대조조）20례。분별채집PE환자화정상만기임신부녀외주혈，채용류식세포술검측Treg이급활화기성상관（CD45RO、HLA- DR）、귀소상관（CCR4、CCR6、CD103）、공능상관（ICOS、CTLA-4、Galectin 1）화생존상관（PD1화CD95）표기분자적표체。채용면역억제실험검측외주혈중 Treg 적면역억제공능。결과관찰조환자외주혈CD4+CD25+세포적비례[(5.87±3.34)%]저우대조조[（6.65±1.18）%]，단차이무통계학의의。면역억제실험현시관찰조환자외주혈중Treg대CD4+CD25-T세포적증치억제작용여대조조무통계학차이。통과대Treg다개표기분자적검측진일보발현，ICOS화CCR6재관찰조Treg상적표체수평현저저우대조조Treg（균P＜0.01）；CCR4화Galectin 1적표체수평교대조조저，이CD45RO、HLA- DR、CD103、CTLA、PD1화CD95적표체수평교대조조고，단량조간차이균무통계학의의（균P＞0.05）。결론 Treg아군비례실조가능시도치자간전기발생적중요인소。
Objective To investigate the changes of regulatory T cel s(CD4+CD25+Treg) in peripheral blood of patients with preeclampsia(PE). Methods Peripheral blood of 30 patients with PE and 20 normal gravidas with late pregnancy were en-rol ed. The expression of CD4+CD25+Treg and its markers related to activation (CD45RO, HLA- DR), homing (CCR4, CCR6, CD103), function (ICOS, CTLA- 4, Galectin 1) and survival (PD 1 and CD95) were detected using flow cytometry. Immunosup-pression function of Treg in vitro was detected by immunosuppression assay, Results The proportion of CD4+CD25+Treg a-mong CD4+T cel s was 5.87±3.34%in patients with PE, lower than that in control group (6.65±1.18%, P>0.05). Immunosup-pression assay showed similar suppression power of Treg from the two groups. The expression of ICOS and CCR6 on Treg in patients with PE was significantly lower than that in normal pregnancy subjects (P<0.01). The expression of CCR4 and Galectin 1 was decreased but that of CD45RO, HLA- DR, CD103, CTLA, PD1 and CD95 was increased in patients with PE. However, the differences between the two groups were not significant. Conclusion Imbalance of Treg subsets may be an important factor leading to the occurrence of preeclampsia.
