CAJ | 학술논문

目的：研究miR-21和ERK1/2在食管鳞状细胞癌(esophageal squamous carcinoma，ESCC)组织中的表达并分析二者的临床意义。方法：原位杂交技术和免疫组化方法分别检测80例食管鳞状细胞癌及癌旁正常组织中miR-21和ERK1/2的表达，Spearman相关统计方法分析miR-21和ERK1/2表达的相关性，并分析二者与ESCC患者临床病理资料的关系。结果：miR-21和ERK1/2在ESCC组织中的阳性表达率和表达量都显著高于癌旁正常组织，尤其在有淋巴结转移的ESCC组织中显著增高，但在ESCC不同性别、年龄、民族、病理类型、肿瘤侵及范围和肿瘤大小间的差异无统计学意义。进一步分析发现miR-21与磷酸化的ERK1/2(p-ERK1/2)在ESCC及癌旁正常组织的表达呈正相关，相关系数为0.570。结论：miR-21和p-ERK1/2与ESCC发生发展密切相关，二者可能协同通过促进肿瘤细胞淋巴结转移增加ESCC的恶性程度。
목적：연구miR-21화ERK1/2재식관린상세포암(esophageal squamous carcinoma，ESCC)조직중적표체병분석이자적림상의의。방법：원위잡교기술화면역조화방법분별검측80례식관린상세포암급암방정상조직중miR-21화ERK1/2적표체，Spearman상관통계방법분석miR-21화ERK1/2표체적상관성，병분석이자여ESCC환자림상병리자료적관계。결과：miR-21화ERK1/2재ESCC조직중적양성표체솔화표체량도현저고우암방정상조직，우기재유림파결전이적ESCC조직중현저증고，단재ESCC불동성별、년령、민족、병리류형、종류침급범위화종류대소간적차이무통계학의의。진일보분석발현miR-21여린산화적ERK1/2(p-ERK1/2)재ESCC급암방정상조직적표체정정상관，상관계수위0.570。결론：miR-21화p-ERK1/2여ESCC발생발전밀절상관，이자가능협동통과촉진종류세포림파결전이증가ESCC적악성정도。
Objective: To explore the expression of miR-21 and ERK1/2 in esophageal squamous carcinoma (ESCC) tissue and the clinical signiifcance.Methods:MiR-21 and ERK1/2 expression in 80 cases of ESCC tissues and adjacent normal tissues were detected by in situ hybridization and immunohistochemical methods respectively. Relationship between miR-21, ERK1/2 and clinicopathological parameters of 80 cases of ESCC was analysed by Spearman analysis.Results: MiR-21 and ERK1/2 expression rate and number were signiifcantly higher than that of adjacent normal tissue, especially in the ESCC tissues of lymph node metastasis, but have no statistical signiifcance in different gender, age, nationality, ESCC pathological type, tumor invasion and tumor size. Further analysis found that miR-21 expression had obvious positive correlation with p-ERK1/2 in ESCC, the correlation coeffcient is 0.570.Conclusion:MiR-21 and p-ERK1/2 are closely related to the occurrence and developmentof ESCC, the two key moleculars may be coordinated to promote malignant degree of ESCC by lymph node metastasis.