四川农业大学学报
四川農業大學學報
사천농업대학학보
JOURNAL OF SICHUAN AGRICULTURAL UNIVERSITY
2015年
2期
189-198
,共10页
潘峰%张敏敏%董品利%吴卫
潘峰%張敏敏%董品利%吳衛
반봉%장민민%동품리%오위
瓦布贝母%索氏提取%超声提取%GC-MS%化学成分%抗氧化活性
瓦佈貝母%索氏提取%超聲提取%GC-MS%化學成分%抗氧化活性
와포패모%색씨제취%초성제취%GC-MS%화학성분%항양화활성
Fritillaria wabuensis%soxhlet extraction method%ultrasonication extraction%GC-MS%chemical composition%antioxidant activity
【目的】分析瓦布贝母鳞茎乙醚提取物中化学成分及其抗氧化活性。【方法】瓦布贝母鳞茎干、鲜样分别采用索氏提取法和超声提取法以乙醚为溶剂进行提取，并结合 GC-MS 分析其化学成分，用面积归一法研究其相对含量，再用 DPPH 自由基和 ABTS＋自由基清除法评价其抗氧化活性。【结果】超声提取法提取效率较好，从超声鲜样（CX），索氏鲜样（SX），超声干样（CG）和索氏干样（SG）的提取物中分别鉴定出69，58，73和57种成分，分别占其总成分百分比为62.16％，53.70％，65.18％和58.76％，占其总含量百分比为80.01％，68.91％，68.62％和74.89％。所得成分中十六烷酸、亚油酸乙酯、亚油酸和邻苯二甲酸单（2-乙基己基）酯为主要成分。此外，所有样品都表现出一定的自由基清除活性，CX、SX、CG 和 SG 的 DPPH 自由基清除率的 IC50分别为30.66、29.77、15.99和21.11 mg／mL，ABTS＋自由基清除率的 IC50分别为35.72、21.08、23.40和15.45 mg／mL。【结论】不同提取方法和不同样品状态对其主要成分的种类含量影响较小；4种测定样主要成分基本一致，但低含量的成分差别较大；并且瓦布贝母乙醚溶解成分有一定的抗氧化活性；所用 GC-MS 分析方法简便、快速、灵敏度高，结果可进一步促进对瓦布贝母药效物质基础的认识。
【目的】分析瓦佈貝母鱗莖乙醚提取物中化學成分及其抗氧化活性。【方法】瓦佈貝母鱗莖榦、鮮樣分彆採用索氏提取法和超聲提取法以乙醚為溶劑進行提取，併結閤 GC-MS 分析其化學成分，用麵積歸一法研究其相對含量，再用 DPPH 自由基和 ABTS＋自由基清除法評價其抗氧化活性。【結果】超聲提取法提取效率較好，從超聲鮮樣（CX），索氏鮮樣（SX），超聲榦樣（CG）和索氏榦樣（SG）的提取物中分彆鑒定齣69，58，73和57種成分，分彆佔其總成分百分比為62.16％，53.70％，65.18％和58.76％，佔其總含量百分比為80.01％，68.91％，68.62％和74.89％。所得成分中十六烷痠、亞油痠乙酯、亞油痠和鄰苯二甲痠單（2-乙基己基）酯為主要成分。此外，所有樣品都錶現齣一定的自由基清除活性，CX、SX、CG 和 SG 的 DPPH 自由基清除率的 IC50分彆為30.66、29.77、15.99和21.11 mg／mL，ABTS＋自由基清除率的 IC50分彆為35.72、21.08、23.40和15.45 mg／mL。【結論】不同提取方法和不同樣品狀態對其主要成分的種類含量影響較小；4種測定樣主要成分基本一緻，但低含量的成分差彆較大；併且瓦佈貝母乙醚溶解成分有一定的抗氧化活性；所用 GC-MS 分析方法簡便、快速、靈敏度高，結果可進一步促進對瓦佈貝母藥效物質基礎的認識。
【목적】분석와포패모린경을미제취물중화학성분급기항양화활성。【방법】와포패모린경간、선양분별채용색씨제취법화초성제취법이을미위용제진행제취，병결합 GC-MS 분석기화학성분，용면적귀일법연구기상대함량，재용 DPPH 자유기화 ABTS＋자유기청제법평개기항양화활성。【결과】초성제취법제취효솔교호，종초성선양（CX），색씨선양（SX），초성간양（CG）화색씨간양（SG）적제취물중분별감정출69，58，73화57충성분，분별점기총성분백분비위62.16％，53.70％，65.18％화58.76％，점기총함량백분비위80.01％，68.91％，68.62％화74.89％。소득성분중십륙완산、아유산을지、아유산화린분이갑산단（2-을기기기）지위주요성분。차외，소유양품도표현출일정적자유기청제활성，CX、SX、CG 화 SG 적 DPPH 자유기청제솔적 IC50분별위30.66、29.77、15.99화21.11 mg／mL，ABTS＋자유기청제솔적 IC50분별위35.72、21.08、23.40화15.45 mg／mL。【결론】불동제취방법화불동양품상태대기주요성분적충류함량영향교소；4충측정양주요성분기본일치，단저함량적성분차별교대；병차와포패모을미용해성분유일정적항양화활성；소용 GC-MS 분석방법간편、쾌속、령민도고，결과가진일보촉진대와포패모약효물질기출적인식。
Abstrzct:[Objective]The aim of the study was to study the chemical composition of the ether ex-tract from Fritillaria wabuensis and its antioxidant activity.[Method]GC-MS was used to detect the ether extract from the dry and fresh samples of F .wabuensis by soxhlet extraction method (SEM)and ultrasonication extraction (UE),respectively.The relative contents of chemical com-position from F .wabuensis were determinated by area normalization.The antioxidant capacity was evaluated by DPPH and ABTS+ radical scavenging assay.[Results]There was higher extraction efficiency by UE than SEM.69 and 58 kinds of components from the fresh samples by SEM and UE were determined but 73 and 57 kinds of components from dry samples by SEM and UE were determined, respectively. Moreover, the percentages of those components were 62.16%, 53.70%,65.18% and 58.76% in the total number of components as well as 80.01%,68.91%, 68.62% and 74.89% in the total content,respectively.The main components were n-hexadecano-ic acid,linoleic acid ethyl ester,(Z,Z)-9,12-octadecadienoic acid and 1,2-benzenedicarboxylic acid,mono (2-ethylhexyl)ester in all samples.The ether extracts from fresh and dry samples by SEM and UE all showed antioxidant activity (IC50 values were 30.66,29.77,15.99 and 21.11 mg/mL with DPPH;35.72,21.08,23.40 and 15.45 mg/mL with ABTS+ ,respectively).[Con-clusion]The different extract methods and treatments had a relatively little effect on the main components.However,there was obvious difference in the low content ingredients in different samples.Furthermore,the ether extracts from F .wabuensis had certain antioxidant activity.GC-MS is a simple,sensitive and rapid method for determination of the chemical composition.It is helpful for our understanding of F .wabuensis .
