CAJ | 학술논문

【目的】研究泸州老窖窖泥中己酸菌的遗传多样性和系统发育地位，筛选优良高产己酸菌株，为提高新窖酒品质提供优质菌株资源。【方法】采用 BOXAIR-PCR、16S rDNA PCR-RFLP 和代表菌株16S rDNA 序列分析技术等对不同窖龄窖泥中乙酸菌的多样性进行分析。【结果】从不同窖龄窖泥样品中分离获得了43株产己酸细菌；BOXAIR-PCR 分析结果表明，在82.2％的相似性水平处，供试菌株形成了8个 BOX 遗传群；16S rDNA PCR-RFLP将43株供试菌株分为8个遗传群；代表菌株16S rDNA 序列系统发育分析表明，供试菌株分布于3个系统发育分支。【结论】泸州老窖窖泥中己酸菌具有丰富的遗传多样性，这些菌株主要分布于梭菌属（Clostridium）、芽孢杆菌属（Bacillus）和假单胞菌属（Pseudomonas），其中芽孢杆菌属（Bacillus）为优势属。
【목적】연구로주로교교니중기산균적유전다양성화계통발육지위，사선우량고산기산균주，위제고신교주품질제공우질균주자원。【방법】채용 BOXAIR-PCR、16S rDNA PCR-RFLP 화대표균주16S rDNA 서렬분석기술등대불동교령교니중을산균적다양성진행분석。【결과】종불동교령교니양품중분리획득료43주산기산세균；BOXAIR-PCR 분석결과표명，재82.2％적상사성수평처，공시균주형성료8개 BOX 유전군；16S rDNA PCR-RFLP장43주공시균주분위8개유전군；대표균주16S rDNA 서렬계통발육분석표명，공시균주분포우3개계통발육분지。【결론】로주로교교니중기산균구유봉부적유전다양성，저사균주주요분포우사균속（Clostridium）、아포간균속（Bacillus）화가단포균속（Pseudomonas），기중아포간균속（Bacillus）위우세속。
Abstrzct:[Objective]The aim of this study was to explore the genetic diversity and phylogeny of cultured caproic acid bacteria in the Luzhou Laojiao pit mud and to screen some effective caproic acid strains.[Method]BOXAIR-PCR,16S rDNA PCR-RFLP and 16S rDNA sequencing were used to explore the genetic diversity and phylogeny of caproic acid bacteria in different aged Luzhou Laojiao pit mud.[Results]43 caproic acid strains were isolated from different aged Luzhou Laojiao pit mud;BOXAIR-PCR analysis showed that all strains were 8 BOXAIR-PCR groups at the level of 82.2% similarity.The results of 16S rDNA PCR-RFLP showed that there were eight genotypes among strains.These strains constructed three phylogenic branches.[Con-clusion]These caproic acid bacteria belonged to three different genus strains including Bacillus , Clostridium and Pseudomonas and Bacillus was the predominant group.The genetic diversity of caproic acid bacteria was high in the Luzhou Laojiao pit mud.