CAJ | 학술논문

目的：观察分析采用2代杂交捕获法检测高危人乳头状瘤病毒 DNA（HC2－HPV－DNA）对宫颈癌病变筛查中的应用及准确性。方法选取110例疑似宫颈癌患者为研究对象，分别进行宫颈液基薄层细胞学（LCT）检查、HC2－HPV－DNA 检测及宫颈组织病理活检，观察分析 LCT 检查、HC2－HPV－DNA 检测和二者联合检查阳性率、敏感性、特异性及预测值情况。结果随着宫颈病变严重程度的增加，HC2－HPV－DNA 的阳性率升高，差异有统计学义意（P ＜0．05）， HC2－HPV－DNA 与 LCT 联合检测敏感性、特异性、阳性预测值分别为78．1％、40．0％、95．3％，敏感性较单独检测显著提高（P ＜0．05）。结论HC2－HPV－DNA 是宫颈癌病变筛选的良好参照指标，随病变程度的增加，筛选准确性尤为突出， HC2－HPV－DNA 与 LCT 方法联合运用可提高宫颈癌及癌前病变诊断的准确性。
목적：관찰분석채용2대잡교포획법검측고위인유두상류병독 DNA（HC2－HPV－DNA）대궁경암병변사사중적응용급준학성。방법선취110례의사궁경암환자위연구대상，분별진행궁경액기박층세포학（LCT）검사、HC2－HPV－DNA 검측급궁경조직병리활검，관찰분석 LCT 검사、HC2－HPV－DNA 검측화이자연합검사양성솔、민감성、특이성급예측치정황。결과수착궁경병변엄중정도적증가，HC2－HPV－DNA 적양성솔승고，차이유통계학의의（P ＜0．05）， HC2－HPV－DNA 여 LCT 연합검측민감성、특이성、양성예측치분별위78．1％、40．0％、95．3％，민감성교단독검측현저제고（P ＜0．05）。결론HC2－HPV－DNA 시궁경암병변사선적량호삼조지표，수병변정도적증가，사선준학성우위돌출， HC2－HPV－DNA 여 LCT 방법연합운용가제고궁경암급암전병변진단적준학성。
Objective To observe hybrid capture 2 high-risk human papilloma virus DNA(HC2-HPV-DNA) in cervical lesion screening and accuracy .Methods 110 cases of patients with suspected symptoms of cervical lesion were selected as the research object,and respectively received liquid-based thinlayer cytology test(LCT)inspection,HC2-HPV-DNA testing and cervi-cal tissues pathology biopsy .Positive rate,sensitivity,specificity,and predictive value of LCT,HC2-HPV-DNA testing and the joint inspection were analyzed.Results HC2-HPV-DNA positive rate increased with the increase of cervical lesion severity ,there had statistical difference(P <0.05),sensitivity,specificity,and positive predictive value of HC2-HPV-DNA with LCT joint detection were 78.1%,40% and 95.3%,respectively,the sensitivity significantly increased than single detection (P <0.05).Conclusion HC2-HPV-DNA is a good reference indicator in cervical lesion screening ,the accuracy increases with the increase of the degree of pathological changes,HC2-HPV-DNA and LCT joint detection can improve the accuracy of the diagnosis of cervical cancer and precancerous lesions.