CAJ | 학술논문

目的：研究妊娠滋养细胞肿瘤患者外周血β－HCG mRNA 表达情况，评价其表达与不同临床指标的关系。方法收集28例妊娠滋养细胞肿瘤、15例葡萄胎、10例正常妊娠妇女和10例非妊娠妇女外周血。运用外周血密度梯度离心法提取单个核细胞和滋养细胞后采用 RT－PCR 方法检测四者β－HCG mRNA 表达水平，并与妊娠滋养细胞肿瘤临床指标做相关分析。结果10例非妊娠妇女外周血β－HCG mRNA 表达均阴性，10例正常妊娠妇女外周血中3例见β－HCG mRNA 扩增条带，15例葡萄胎患者外周血中8例见β－HCG mRNA 扩增条带，28例妊娠滋养细胞肿瘤患者外周血中24例见β－HCG mRNA 扩增条带，正常妊娠妇女β－HCG mRNA 表达水平低于葡萄胎、妊娠滋养细胞肿瘤患者（P ＜0．05）。妊娠滋养细胞肿瘤患者外周血β－HCG mRNA 表达与治疗前血β－HCG 水平、肺转移及临床分期有关（P ＜0．05）。28例随访病例中只有1例进展，无复发病例。结论外周血中单个核细胞不表达β－HCG mRNA。正常妊娠、葡萄胎及妊娠滋养细胞肿瘤均有滋养细胞脱落入血。外周血滋养细胞β－HCG mRNA 可能成为评估妊娠滋养细胞肿瘤早期血行转移的有效指标。
목적：연구임신자양세포종류환자외주혈β－HCG mRNA 표체정황，평개기표체여불동림상지표적관계。방법수집28례임신자양세포종류、15례포도태、10례정상임신부녀화10례비임신부녀외주혈。운용외주혈밀도제도리심법제취단개핵세포화자양세포후채용 RT－PCR 방법검측사자β－HCG mRNA 표체수평，병여임신자양세포종류림상지표주상관분석。결과10례비임신부녀외주혈β－HCG mRNA 표체균음성，10례정상임신부녀외주혈중3례견β－HCG mRNA 확증조대，15례포도태환자외주혈중8례견β－HCG mRNA 확증조대，28례임신자양세포종류환자외주혈중24례견β－HCG mRNA 확증조대，정상임신부녀β－HCG mRNA 표체수평저우포도태、임신자양세포종류환자（P ＜0．05）。임신자양세포종류환자외주혈β－HCG mRNA 표체여치료전혈β－HCG 수평、폐전이급림상분기유관（P ＜0．05）。28례수방병례중지유1례진전，무복발병례。결론외주혈중단개핵세포불표체β－HCG mRNA。정상임신、포도태급임신자양세포종류균유자양세포탈락입혈。외주혈자양세포β－HCG mRNA 가능성위평고임신자양세포종류조기혈행전이적유효지표。
Objective To study the expression of β-Human Chorionic Gonadotrophin (β-HCG) in peripheral blood of the patients with gestational trophoblastic tumor ,and its relationship with various clinical indicators .Methods Peripheral blood of 28 cases of gestational trophoblastic tumors ,15 cases of hydatidiform moles,10 cases of normal pregnant women and 10 cases of non-pregnant women were collected .Mononuclear cells and trophoblast cells were extracted from the peripheral blood by density gradient centrifugation method ,then reverse transcription-polymerase chain reaction (RT-PCR) was used to detect the expression of β-HCG mRNA of the 4 groups.The correlation between the expression of β-hcg mRNA and the clinical indicators of gestational trophoblastic tumor was analyzed .Results The expression of β-HCG mRNA in peripheral blood of 10 cases of non-pregnant women was negative.In 10 cases of normal pregnant women ,3 women showed β-HCG mRNA amplified bands.There were 8 cases showed β-HCG mRNA amplified bands in 15 cases of hydatidiform moles.In 28 cases of gestational trophoblastic tumors ,β-HCG mRNA amplified bands were seen in 24 patients.The β-HCG mRNA expression of normal pregnancy was lower than that of hyda -tidiform moles and gestational trophoblastic tumors (P <0.05).There were correlation between the expression of β-HCG mRNA and the serumβ-HCG levels before treatment,lung metastasis and clinical stage (P <0.05).Only 1 case showed progression a-mong 28 cases during follow-up.No case recurred.Conclusion Mononuclear cells in peripheral blood do not express β-HCG mRNA.Trophoblast cells can disseminate into peripheral blood in normal pregnancy ,hydatidiform mole and gestational tropho -blastic tumor.β-HCG mRNA of trophoblast cell in peripheral blood may be an effective marker in assessing the early hematoge -nous metastasis of gestational trophoblastic tumor .