应用化工
應用化工
응용화공
APPLIED CHEMICAL INDUSTRY
2015年
6期
1154-1156
,共3页
赵红玲%高杨%李云红%尹志峰%王良友
趙紅玲%高楊%李雲紅%尹誌峰%王良友
조홍령%고양%리운홍%윤지봉%왕량우
内吗啡肽-2%固相合成%Rink Amide树脂
內嗎啡肽-2%固相閤成%Rink Amide樹脂
내마배태-2%고상합성%Rink Amide수지
endomorphin-2%solid-phase synthesis%Rink Amide resin
采用多肽固相合成法,以Rink Amide树脂为载体,Fmoc保护氨基酸为原料,以DIC/HOBt为缩合体系,按照内吗啡肽-2的肽序列,从C端向N端偶联,肽树脂经三氟乙酸裂解,得到内吗啡肽-2粗品,纯度为98.5%,再经制备型反相高效液相色谱法纯化和转盐,得到醋酸内吗啡肽-2纯品,纯度>99.0%,收率为80%。
採用多肽固相閤成法,以Rink Amide樹脂為載體,Fmoc保護氨基痠為原料,以DIC/HOBt為縮閤體繫,按照內嗎啡肽-2的肽序列,從C耑嚮N耑偶聯,肽樹脂經三氟乙痠裂解,得到內嗎啡肽-2粗品,純度為98.5%,再經製備型反相高效液相色譜法純化和轉鹽,得到醋痠內嗎啡肽-2純品,純度>99.0%,收率為80%。
채용다태고상합성법,이Rink Amide수지위재체,Fmoc보호안기산위원료,이DIC/HOBt위축합체계,안조내마배태-2적태서렬,종C단향N단우련,태수지경삼불을산렬해,득도내마배태-2조품,순도위98.5%,재경제비형반상고효액상색보법순화화전염,득도작산내마배태-2순품,순도>99.0%,수솔위80%。
Endomorphin-2 was synthesized successfully from C-terminal to N-terminal according to the a-mino acids sequence with the raw material of Fmoc-amino acids and Rink Amide resin as the vector. DIC/HOBt as the coupling reagent. The crude peptide of endomorphin-2 was obtained by cracking by TFA from the resin,the purity of crude product was 98. 5%. Then the target product of endomorphin-2 was achieved after purification and turning salt by preparative reversed-phase high performance liquid chromatography. The purity of refined peptide was more than 99. 0%,and the yield was reach 80%.