应用化工
應用化工
응용화공
APPLIED CHEMICAL INDUSTRY
2015年
6期
1140-1142
,共3页
青荚叶%木犀草素%肉桂酸%HPLC%紫外检测器
青莢葉%木犀草素%肉桂痠%HPLC%紫外檢測器
청협협%목서초소%육계산%HPLC%자외검측기
Helwingia%luteolin%cinnamic acid%HPLC%UV detector
建立测定青荚叶中木犀草素、肉桂酸含量的HPLC测定方法。采用反相高效液相色谱法( RP-HPLC),色谱柱为Thermo C18柱(250 mm ×4.6 mm,5μm),测定木犀草素:流动相为甲醇∶0.2%磷酸水溶液(55∶45),流速1.0 mL/min,进样量20μL,检测波长350 nm;测定肉桂酸:流动相为甲醇∶1.67%冰醋酸水溶液(50∶50),流速1.0 mL/min,进样量20μL,检测波长270 nm。结果表明,木犀草素进样量在3.4~54.4μg范围内,其峰面积与浓度呈良好的线性关系(R2=0.9999),样品回收率为99.67%,肉桂酸进样量在0.24~2.4μg范围内,其峰面积与浓度呈良好的线性关系(R2=0.9994),样品回收率为93.64%。方法专属性强,准确可靠,可用于青荚叶中木犀草素、肉桂酸的含量测定。
建立測定青莢葉中木犀草素、肉桂痠含量的HPLC測定方法。採用反相高效液相色譜法( RP-HPLC),色譜柱為Thermo C18柱(250 mm ×4.6 mm,5μm),測定木犀草素:流動相為甲醇∶0.2%燐痠水溶液(55∶45),流速1.0 mL/min,進樣量20μL,檢測波長350 nm;測定肉桂痠:流動相為甲醇∶1.67%冰醋痠水溶液(50∶50),流速1.0 mL/min,進樣量20μL,檢測波長270 nm。結果錶明,木犀草素進樣量在3.4~54.4μg範圍內,其峰麵積與濃度呈良好的線性關繫(R2=0.9999),樣品迴收率為99.67%,肉桂痠進樣量在0.24~2.4μg範圍內,其峰麵積與濃度呈良好的線性關繫(R2=0.9994),樣品迴收率為93.64%。方法專屬性彊,準確可靠,可用于青莢葉中木犀草素、肉桂痠的含量測定。
건립측정청협협중목서초소、육계산함량적HPLC측정방법。채용반상고효액상색보법( RP-HPLC),색보주위Thermo C18주(250 mm ×4.6 mm,5μm),측정목서초소:류동상위갑순∶0.2%린산수용액(55∶45),류속1.0 mL/min,진양량20μL,검측파장350 nm;측정육계산:류동상위갑순∶1.67%빙작산수용액(50∶50),류속1.0 mL/min,진양량20μL,검측파장270 nm。결과표명,목서초소진양량재3.4~54.4μg범위내,기봉면적여농도정량호적선성관계(R2=0.9999),양품회수솔위99.67%,육계산진양량재0.24~2.4μg범위내,기봉면적여농도정량호적선성관계(R2=0.9994),양품회수솔위93.64%。방법전속성강,준학가고,가용우청협협중목서초소、육계산적함량측정。
To establish a HPLC method for content determination of luteolin in Helwingia and cinnamic acid. Using reversed-phase high performance liquid chromatography ( RP-HPLC ) , the chromatographic column was Thermo C18 column (250 mm × 4. 6 mm,5 μm),mobile phase of methanol determination of luteolin∶0. 2% phosphoric acid solution (55∶45),the flow rate was 1. 0 mL/min,the injection volume was 20 μL, the detection wavelength was 350 nm;determination of cinnamic acid∶the mobile phase of methanol∶1. 67% glacial acetic acid aqueous solution (50∶50),the flow rate was 1. 0 mL/min,the injec-tion volume was 20 μL,the detection wavelength was 270 nm. Results showed that luteolin in the range of 3. 4~54. 4 μg, there was a good linear relationship between the peak area and concentration ( R2 =0. 999 9),sample recovery rate was 99. 67%,cinnamic acid was in the range of 0. 24~2. 4μg,there was a good linear relationship between the peak area and concentration (R2 =0. 999 4),sample recovery rate was 93. 64%. This method is specific,accurate and reliable,and can be used for content determination of luteolin in Helwingia and cinnamic acid.