CAJ | 학술논문

目的：观察大蒜素对全脑缺血再灌注损伤大鼠海马凋亡相关蛋白表达的影响，进一步探讨大蒜素的脑保护机制。方法雄性Wistar大鼠30只，随机分为5组：假手术组（ Sham 组）；缺血再灌注组（ IR组）；缺血再灌注＋大蒜素10 mg／kg（All－10 mg）、20 mg／kg（All－20 mg）、30 mg／kg （All－30 mg）组，夹闭两侧颈总动脉8 min再灌注24 h建立大鼠全脑缺血再灌注模型，取海马组织硫堇染色观察海马组织学改变及存活神经元密度；免疫组织化学染色测定海马CA1区Bcl－2、Bax蛋白的表达。结果与Sham组相比，IR组海马CA1区神经元密度明显降低，Bax蛋白表达明显增加（均P＜0．05）；与IR组相比，大蒜素处理各组海马CA1区神经元密度明显增多，Bcl－2蛋白表达明显增加，Bax蛋白表达明显减少，其中以All－20 mg组变化最显著（均P＜0．05）。结论大蒜素可以通过影响凋亡相关蛋白的表达而抑制细胞凋亡，从而发挥脑保护作用。
목적：관찰대산소대전뇌결혈재관주손상대서해마조망상관단백표체적영향，진일보탐토대산소적뇌보호궤제。방법웅성Wistar대서30지，수궤분위5조：가수술조（ Sham 조）；결혈재관주조（ IR조）；결혈재관주＋대산소10 mg／kg（All－10 mg）、20 mg／kg（All－20 mg）、30 mg／kg （All－30 mg）조，협폐량측경총동맥8 min재관주24 h건립대서전뇌결혈재관주모형，취해마조직류근염색관찰해마조직학개변급존활신경원밀도；면역조직화학염색측정해마CA1구Bcl－2、Bax단백적표체。결과여Sham조상비，IR조해마CA1구신경원밀도명현강저，Bax단백표체명현증가（균P＜0．05）；여IR조상비，대산소처리각조해마CA1구신경원밀도명현증다，Bcl－2단백표체명현증가，Bax단백표체명현감소，기중이All－20 mg조변화최현저（균P＜0．05）。결론대산소가이통과영향조망상관단백적표체이억제세포조망，종이발휘뇌보호작용。
Objective To observe the effect of allicin on apoptosis-related protein in hippocampus of rats after global cerebral ischemic reperfusion injury , and detect the neuroprotective mechanism of allicin .Methods Thirty male Wistar rats were randomly divided into 5 groups: Sham operation group ( Sham group );ischemia reperfusion group(IR group);ischemia reperfusion+Allicin 10 mg/kg group(All-10 mg group), ischemia reperfusion+20 mg/kg group(All-20 mg group), ischemia reperfusion+30 mg/kg group(All-30 mg group).The rat model of cerebral ischemia reperfusion was established by occluding both sides of commom carotid arteries for 8 min and reperfusing for 24 h, hippocampal tissue was used to observe the histological changes and survival of hippocampal neuron density by Giemsa staining, expression of Bcl-2, Bax-immunoreactive protein in hippocampal CA 1 area was measured by immun-ohistochemical staining .Results Compared with the sham group , the neuron density in hippocampus CA 1 area of IR group was significantly decreased , the expression of Bax protein was significantly increased ( all P<0.05);compared with IR group,the neuron density in hippocampus CA1 area of All-10 mg,All-20 mg,All-30 mg group was signi-ficantly increased , the expression of Bcl-2 protein was significantly increased ,the expression of Bax protein was signi-ficantly decreased,the change in All-20 mg group was most obviously(all P<0.05).Conclusion Allicin can inhibit apoptosis through affecting the expression of apoptosis related proteins , play an important role in brain protection .