CAJ | 학술논문

目的：探讨Aβ1-42海马直接注射对大鼠海马细胞的影响及黄精多糖干预的作用。方法：将45只SD雄性大鼠分为3组：假手术组、Aβ模型组(Aβ组)和黄精多糖干预组(PP组)。Aβ组直接注射Aβ1-42于大鼠海马组织；PP组用16%黄精多糖溶液给予大鼠连续灌胃45 d。采用HE和甲醇刚果红染色观察Aβ及黄精多糖干预对海马组织细胞的影响。结果：海马的形态学在大鼠的假手术组没有显著变化，在PP组大鼠基本正常，Aβ组的锥体细胞层明显减少，细胞排列稀疏、不规则地变小，可见核固缩、空泡变性细胞。Aβ组相比于PP组，海马组织细胞出现明显Aβ沉积；Aβ组大鼠的阳性斑块数量高于PP组大鼠。结论：Aβ海马注射可以模拟大鼠海马组织阿尔茨海默病病理改变；黄精多糖可显著改善阿尔茨海默病大鼠海马的病理改变。
목적：탐토Aβ1-42해마직접주사대대서해마세포적영향급황정다당간예적작용。방법：장45지SD웅성대서분위3조：가수술조、Aβ모형조(Aβ조)화황정다당간예조(PP조)。Aβ조직접주사Aβ1-42우대서해마조직；PP조용16%황정다당용액급여대서련속관위45 d。채용HE화갑순강과홍염색관찰Aβ급황정다당간예대해마조직세포적영향。결과：해마적형태학재대서적가수술조몰유현저변화，재PP조대서기본정상，Aβ조적추체세포층명현감소，세포배렬희소、불규칙지변소，가견핵고축、공포변성세포。Aβ조상비우PP조，해마조직세포출현명현Aβ침적；Aβ조대서적양성반괴수량고우PP조대서。결론：Aβ해마주사가이모의대서해마조직아이자해묵병병리개변；황정다당가현저개선아이자해묵병대서해마적병리개변。
Objective: To determine the effect of polygona-polysaccharose (PP) on learning and memory ability in rats with Alzheimer’s disease (AD). Methods: Forty ifve Sprague-Dawley rats were assigned into 3 groups. Rats in the sham-operated group were injected with normal saline. Rats in the Aβ group were injected with Aβ1-42. Rats in the PP group were injected with 16% PP solution for 45 days consecutively. hTe Morris water maze was used to investigate the ability of learning and memory in the rats. hTe effect of Aβ and PP on the hippocampus cells was observed by HE and Congo red staining of methanol. Results: Rats in the sham-operated group had no obvious morphological change; and morphology of rats in the PP group was basicaly normal. The layer of pyramidal cells in the Aβ group was decreased. hTe cells appeared sparse and irregular and became smaller. Karyopyknosis and vacuolardegeneration cells were also found. More positive staining materials aggradated in the Aβ group compared with the PP group by Congo red staining (P<0.05). Conclusion: Aβ infusion into the hippocampus results in the impairment of the neuronal degeneration in the rats, which shows similar characterizations of AD. PP can reduce the deposition of Aβ in the hippocampus.