齐鲁工业大学学报
齊魯工業大學學報
제로공업대학학보
Journal of Shandong Institute of Light Industry (Natural Science Edition)
2015年
2期
29-32
,共4页
10-HDA%工蜂%10-羟基癸酸%高效液相色谱
10-HDA%工蜂%10-羥基癸痠%高效液相色譜
10-HDA%공봉%10-간기계산%고효액상색보
10-HDA%bees%long-chain fatty acids%HPLC
在工蜂饲料中添加10-羟基癸酸(简称10-HDAA),采用高效液相色谱( HPLC)法,检测工蜂头部腺体中10-羟基-2癸烯酸(10-HDA)的含量,探讨不同浓度的10-羟基癸酸对工蜂合成10-HDA的影响。结果显示,在蜜蜂花粉饲料中添加10-HDAA,在一定程度上能够促进工蜂头部10-HDA的合成；添加量不同,对工蜂上颚腺分泌10-HDA的作用效果也有较大差异。添加1％10-HDAA效果较好；花粉饲料中添加10-HDAA能使工蜂10-HDA分泌高峰由原来的20日龄提前到15日龄。
在工蜂飼料中添加10-羥基癸痠(簡稱10-HDAA),採用高效液相色譜( HPLC)法,檢測工蜂頭部腺體中10-羥基-2癸烯痠(10-HDA)的含量,探討不同濃度的10-羥基癸痠對工蜂閤成10-HDA的影響。結果顯示,在蜜蜂花粉飼料中添加10-HDAA,在一定程度上能夠促進工蜂頭部10-HDA的閤成；添加量不同,對工蜂上顎腺分泌10-HDA的作用效果也有較大差異。添加1％10-HDAA效果較好；花粉飼料中添加10-HDAA能使工蜂10-HDA分泌高峰由原來的20日齡提前到15日齡。
재공봉사료중첨가10-간기계산(간칭10-HDAA),채용고효액상색보( HPLC)법,검측공봉두부선체중10-간기-2계희산(10-HDA)적함량,탐토불동농도적10-간기계산대공봉합성10-HDA적영향。결과현시,재밀봉화분사료중첨가10-HDAA,재일정정도상능구촉진공봉두부10-HDA적합성；첨가량불동,대공봉상악선분비10-HDA적작용효과야유교대차이。첨가1％10-HDAA효과교호；화분사료중첨가10-HDAA능사공봉10-HDA분비고봉유원래적20일령제전도15일령。
10-hydroxy decanoic acid (10-HDAA) were added in the worker bees feed,and then the content of 10-Hydroxy-2-Decenoicacid (10-HAD) in the glands of worker bees’ heads were detected by HPLC,to explore effects of different concentrations of 10-HDAA for worker bees to synthesize 10-HDA. The results showed that adding 10-HDAA into the pollen feed could promote the synthesis of 10-HAD in the heads of worker bees in a certain extent. Different amount of addition could make big difference of effects for worker bees mandibular gland to secrete 10-HDA,and adding 1% of 10-HDAA was better. With addition of 10-HDAA in the pollen feed,the 10-HDA peak secretion of worker bees was changed from the original age of 20 days to 15 days of age in advance.
