中华医学杂志
中華醫學雜誌
중화의학잡지
National Medical Journal of China
2015年
24期
1941-1943
,共3页
石涛%卫江鹏%刘刚%韩梅%刘彤
石濤%衛江鵬%劉剛%韓梅%劉彤
석도%위강붕%류강%한매%류동
结肠炎,溃疡性%β防御素%咬合蛋白%免疫球蛋白A,分泌
結腸炎,潰瘍性%β防禦素%咬閤蛋白%免疫毬蛋白A,分泌
결장염,궤양성%β방어소%교합단백%면역구단백A,분비
Colitis,ulcerative%Beta-defensins%Occludin%Immunoglobulin A,secretory
目的 观察溃疡性结肠炎(UC)患者的肠道屏障功能,并探讨其临床意义.方法 收集2010年3月至2013年6月天津医科大学总医院诊断的200例UC患者肠黏膜标本,以40份正常肠黏膜组织为对照.200例UC患者依据Mayo评分分为轻度(68例)、中度(70例)和重度(62例).以咬合蛋白(occludin)、分泌型免疫球蛋白A(sIgA)和β防御素(β-defensin)分别代表肠道机械屏障、免疫屏障和化学屏障,采用免疫组化法检测肠黏膜组织中3种蛋白表达,并与相关临床指标进行统计学分析.结果 与对照相比,UC患者肠黏膜中occludin、sIgA表达显著减少(28.53％±2.21％比44.28％±1.94％,19.26％±1.09％比28.36％±2.36％,均P＜0.05),β-defensin表达显著增加(29.13％±1.75％比19.71％±1.83％,P＜0.05).轻、中、重度UC患者间肠黏膜occludin、β-defensin表达差异均有统计学意义(均P＜0.05);随着UC分级增加occludin表达明显减少(r=-0.753,P＜0.05),β-defensin表达明显增多(r=0.698,P＜0.05).而轻、中、重度UC患者间sIgA表达差异无统计学意义(P＞0.05).UC患者中3种蛋白在不同性别、年龄、病变部位的表达差异无统计学意义(均P ＞0.05).结论 UC患者出现肠道屏障功能损伤,随着临床分级增加,肠道机械屏障、化学屏障损伤更加明显.
目的 觀察潰瘍性結腸炎(UC)患者的腸道屏障功能,併探討其臨床意義.方法 收集2010年3月至2013年6月天津醫科大學總醫院診斷的200例UC患者腸黏膜標本,以40份正常腸黏膜組織為對照.200例UC患者依據Mayo評分分為輕度(68例)、中度(70例)和重度(62例).以咬閤蛋白(occludin)、分泌型免疫毬蛋白A(sIgA)和β防禦素(β-defensin)分彆代錶腸道機械屏障、免疫屏障和化學屏障,採用免疫組化法檢測腸黏膜組織中3種蛋白錶達,併與相關臨床指標進行統計學分析.結果 與對照相比,UC患者腸黏膜中occludin、sIgA錶達顯著減少(28.53％±2.21％比44.28％±1.94％,19.26％±1.09％比28.36％±2.36％,均P＜0.05),β-defensin錶達顯著增加(29.13％±1.75％比19.71％±1.83％,P＜0.05).輕、中、重度UC患者間腸黏膜occludin、β-defensin錶達差異均有統計學意義(均P＜0.05);隨著UC分級增加occludin錶達明顯減少(r=-0.753,P＜0.05),β-defensin錶達明顯增多(r=0.698,P＜0.05).而輕、中、重度UC患者間sIgA錶達差異無統計學意義(P＞0.05).UC患者中3種蛋白在不同性彆、年齡、病變部位的錶達差異無統計學意義(均P ＞0.05).結論 UC患者齣現腸道屏障功能損傷,隨著臨床分級增加,腸道機械屏障、化學屏障損傷更加明顯.
목적 관찰궤양성결장염(UC)환자적장도병장공능,병탐토기림상의의.방법 수집2010년3월지2013년6월천진의과대학총의원진단적200례UC환자장점막표본,이40빈정상장점막조직위대조.200례UC환자의거Mayo평분분위경도(68례)、중도(70례)화중도(62례).이교합단백(occludin)、분비형면역구단백A(sIgA)화β방어소(β-defensin)분별대표장도궤계병장、면역병장화화학병장,채용면역조화법검측장점막조직중3충단백표체,병여상관림상지표진행통계학분석.결과 여대조상비,UC환자장점막중occludin、sIgA표체현저감소(28.53％±2.21％비44.28％±1.94％,19.26％±1.09％비28.36％±2.36％,균P＜0.05),β-defensin표체현저증가(29.13％±1.75％비19.71％±1.83％,P＜0.05).경、중、중도UC환자간장점막occludin、β-defensin표체차이균유통계학의의(균P＜0.05);수착UC분급증가occludin표체명현감소(r=-0.753,P＜0.05),β-defensin표체명현증다(r=0.698,P＜0.05).이경、중、중도UC환자간sIgA표체차이무통계학의의(P＞0.05).UC환자중3충단백재불동성별、년령、병변부위적표체차이무통계학의의(균P ＞0.05).결론 UC환자출현장도병장공능손상,수착림상분급증가,장도궤계병장、화학병장손상경가명현.
Objective To discuss the variation of intestinal barrier and its clinical significance in patients with ulcerative colitis(UC).Methods Specimens were obtained from 200 patients of UC and 40 controls respectively from March 2010 to June 2013.UC samples were classified into three groups as mild (n =68),moderate (n =70) and severe (n =62) according to Mayo classification.Immunohistochemistry was performed to examine the expression of occludin,β-defensin and sIgA,which respectively reflect the changes of mechanical barrier,chemical barrier and immune barrier in intestinal tissue.The result and the clinical data were analyzed.Results Compared with controls,the expression of occludin and sIgA significantly decreased (28.53％ ± 2.21％ vs 44.28％ ± 1.94％,19.26％ ± 1.09％ vs 28.36％ ±2.36％,both P ＜ 0.05),β-defensin significantly increased (29.13 ％ ± 1.75 ％ 比 19.71％ ± 1.83 ％,P ＜ 0.05) in UC group.There was a significant difference of occludin levels among different active subgroups (P ＜ 0.05),and the levels decreased significantly with the increase of UC classification (r =-0.753,P ＜ 0.05).β-defensin levels were significant differently among different active subgroups (P ＜ 0.05),and the levels increased significantly with the increase of UC classification (r =0.698,P ＜ 0.05).There was no significant difference in the sIgA levels among the three stages (P ＞ 0.05).In different gender,age and lesion site,there was no statistically significant difference in expression of three kinds of protein (all P ＞ 0.05).Conclusion UC patients exist intestinal barrier function injury,with the increase of UC classification,the damages of intestinal mechanical barrier and chemical barrier were more obvions.
