CAJ | 학술논문

为探明钠钾泵（Na＋-K＋-ATP 酶）是否为丁香酚作用的靶位之一，以了解丁香酚的作用机制和进一步开发利用提供参考，将体重900～1000 g、体长40～45 cm 的鲤鱼随机分为对照组（10尾，不含丁香酚乳剂的水溶液）和丁香酚处理组（30尾），测定其不同麻醉时期（诱导期、麻醉期和恢复期）鲤鱼大脑、中脑、间脑、小脑、延脑和脊髓中 Na＋-K＋-ATP 酶的活性。结果表明：经丁香酚麻醉后不同麻醉时期鲤鱼各脑和脊髓中 Na＋-K＋-ATP 酶活性均不同程度下降，丁香酚的抑制作用随麻醉程度加深而加强，在麻醉期时抑制作用最强，与对照组比，丁香酚处理组各脑和脊髓中酶活性在麻醉期性均极显著下降（P ＜0．01）；与麻醉期比，恢复期除间脑和延脑外，其余各脑组织和脊髓中酶活性均呈极显著升高（P ＜0．01）。在麻醉的各时期丁香酚对鲤鱼各脑和脊髓中的 Na＋-K＋-ATP 酶有明显的抑制作用，对鲤鱼的麻醉作用可能与其抑制脑 Na＋-K＋-ATP 酶的活性有关。
위탐명납갑빙（Na＋-K＋-ATP 매）시부위정향분작용적파위지일，이료해정향분적작용궤제화진일보개발이용제공삼고，장체중900～1000 g、체장40～45 cm 적리어수궤분위대조조（10미，불함정향분유제적수용액）화정향분처리조（30미），측정기불동마취시기（유도기、마취기화회복기）리어대뇌、중뇌、간뇌、소뇌、연뇌화척수중 Na＋-K＋-ATP 매적활성。결과표명：경정향분마취후불동마취시기리어각뇌화척수중 Na＋-K＋-ATP 매활성균불동정도하강，정향분적억제작용수마취정도가심이가강，재마취기시억제작용최강，여대조조비，정향분처리조각뇌화척수중매활성재마취기성균겁현저하강（P ＜0．01）；여마취기비，회복기제간뇌화연뇌외，기여각뇌조직화척수중매활성균정겁현저승고（P ＜0．01）。재마취적각시기정향분대리어각뇌화척수중적 Na＋-K＋-ATP 매유명현적억제작용，대리어적마취작용가능여기억제뇌 Na＋-K＋-ATP 매적활성유관。
The Na+-K+-ATPase activity in brain, midbrain, interbrain, cerebellum, medulla oblongata and spinal cord of Cyprinus carpio treated with eugenol at different anesthesia stages (induction stage,narcosis stage and restoration stage)was detected to probe the effect of eugenol on Na+-K+-ATPase activity and provide a reference for further development and utilization of eugenol.The results showed that the Na+-K+-ATPase activity in brain,midbrain,interbrain,cerebellum,medulla oblongata and spinal cord of C.carpio treated with eugenol reduces at different anesthesia stages to varying degrees, and the inhibition effect of eugenol increases with increase of anesthesia degree.That is,the inhibition effect of eugenol is the maximum at narcosis stage.The Na+-K+-ATPase activity in brain,midbrain, interbrain,cerebellum,medulla oblongata and spinal cord of Cyprinus carpio Linnaeus treated with eugenol at anesthesia stages all reduces very significantly (P <0.01)compared with CK.The Na+-K+-ATPase activity of brain,midbrain,cerebellum and spinal cord of C.carpio treated with eugenol at restoration stage increases very significantly (P <0.01)compared with at narcosis stage.Eugenol has the obvious inhibition effect on Na+-K+-ATPase activity in brain,midbrain,interbrain,cerebellum,medulla oblongata and spinal cord of C.carpio at three anesthesia stages,which indicates that the anesthesia effect of eugenol may inhibit Na+-K+-ATPase activity in brain of C.carpio.